Zobrazeno 1 - 10
of 75
pro vyhledávání: '"Allen R Buskirk"'
Publikováno v:
Nature Communications, Vol 15, Iss 1, Pp 1-10 (2024)
Abstract Translation elongation is essential for maintaining cellular proteostasis, and alterations in the translational landscape are associated with a range of diseases. Ribosome profiling allows detailed measurements of translation at the genome s
Externí odkaz:
https://doaj.org/article/4a19ce39273348e8a8e77454bb363d00
Autor:
Isao Masuda, Jae-Yeon Hwang, Thomas Christian, Sunita Maharjan, Fuad Mohammad, Howard Gamper, Allen R Buskirk, Ya-Ming Hou
Publikováno v:
eLife, Vol 10 (2021)
N1-methylation of G37 is required for a subset of tRNAs to maintain the translational reading-frame. While loss of m1G37 increases ribosomal +1 frameshifting, whether it incurs additional translational defects is unknown. Here, we address this questi
Externí odkaz:
https://doaj.org/article/a833a1e94d0c41389028df927ab81e6b
Publikováno v:
eLife, Vol 9 (2020)
We used ribosome profiling to characterize the biological role of ribosome recycling factor (RRF) in Escherichia coli. As expected, RRF depletion leads to enrichment of post-termination 70S complexes in 3′-UTRs. We also observe that elongating ribo
Externí odkaz:
https://doaj.org/article/68d9d2665f7c444f851c86fd50165be0
Publikováno v:
eLife, Vol 9 (2020)
Shine-Dalgarno (SD) motifs are thought to play an important role in translational initiation in bacteria. Paradoxically, ribosome profiling studies in E. coli show no correlation between the strength of an mRNA’s SD motif and how efficiently it is
Externí odkaz:
https://doaj.org/article/8627f1244caa4abab56f0778a0beb878
Publikováno v:
mBio, Vol 10, Iss 2 (2019)
ABSTRACT Small proteins consisting of 50 or fewer amino acids have been identified as regulators of larger proteins in bacteria and eukaryotes. Despite the importance of these molecules, the total number of small proteins remains unknown because conv
Externí odkaz:
https://doaj.org/article/01f8e0e61cfc4284a906889e9ea5bfc7
Publikováno v:
eLife, Vol 8 (2019)
In eukaryotes, ribosome profiling provides insight into the mechanism of protein synthesis at the codon level. In bacteria, however, the method has been more problematic and no consensus has emerged for how to best prepare profiling samples. Here, we
Externí odkaz:
https://doaj.org/article/fc7e08537e2f49749251cf62325c35b7
Translation elongation is essential for maintaining cellular proteostasis, and alterations in the translational landscape are associated with a range of diseases. Ribosome profiling allows detailed measurement of translation at genome scale. However,
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::f484753f032f5bf7a48d5843b63a1c63
https://doi.org/10.1101/2023.04.24.538053
https://doi.org/10.1101/2023.04.24.538053
Publikováno v:
Cell Reports, Vol 14, Iss 4, Pp 686-694 (2016)
The rate of protein synthesis varies according to the mRNA sequence in ways that affect gene expression. Global analysis of translational pausing is now possible with ribosome profiling. Here, we revisit an earlier report that Shine-Dalgarno sequence
Externí odkaz:
https://doaj.org/article/223274cd3c7547c0867397ade3c3d301
Autor:
Heather A. Feaga, Hye-Rim Hong, Cassidy R. Prince, Ananda Rankin, Allen R. Buskirk, Jonathan Dworkin
Publikováno v:
Journal of Bacteriology. 205
Elongation factor P (EF-P) is a universally conserved translation factor that prevents ribosome stalling at amino acids that form peptide bonds with low efficiency, particularly polyproline tracts. Phenotypes associated with EF-P deletion are pleiotr
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::5eeb2e932372d2aa797c86c7cb5b0566
https://doi.org/10.1128/jb.00370-22
https://doi.org/10.1128/jb.00370-22
Publikováno v:
Cell Reports, Vol 11, Iss 1, Pp 13-21 (2015)
Ribosome profiling is a powerful method for globally assessing the activity of ribosomes in a cell. Despite its application in many organisms, ribosome profiling studies in bacteria have struggled to obtain the resolution necessary to precisely defin
Externí odkaz:
https://doaj.org/article/ecb7728168d248dc9a6ccf1c7c222dd3