Zobrazeno 1 - 10
of 909
pro vyhledávání: '"Allen Institute for Brain Science"'
Isolation of nuclei from frozen adult human brain tissue or thawed and microdissected brain tissue sections for RNA-seq analysis. Note: Research reported in this publication was supported by the National Institute Of Mental Health of the National Ins
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::24053312a1687ea73e31676967b9f729
https://doi.org/10.17504/protocols.io.yxmvmkerng3p/v2
https://doi.org/10.17504/protocols.io.yxmvmkerng3p/v2
This protocol is used to produce crude preps of AAV of any serotype. Note: Research reported in this publication was supported by the National Institute Of Mental Health of the National Institutes of Health under Award Numbers U19MH114830, RF1MH11412
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::f2ce6d08507db431a573a48712aee103
https://doi.org/10.17504/protocols.io.yxmvmx7r9l3p/v5
https://doi.org/10.17504/protocols.io.yxmvmx7r9l3p/v5
Protocol is used to profile all the open chromatin regions at a single nuclei level through the rapid generation of NGS-ready libraries from a pool of transposed nuclei.
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::586fa8ace41c0cde6f18cff83f16b87a
https://doi.org/10.17504/protocols.io.b93br8in
https://doi.org/10.17504/protocols.io.b93br8in
Protocol is used to profile all the open chromatin regions at a single nuclei level through the rapid generation of NGS-ready libraries, and for rapid generation of 3' transcriptomic-NGS-ready-single-cell-libraries from the same pool of transposed nu
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::174e20a1abca5b00eefb612e4cb5d406
https://doi.org/10.17504/protocols.io.bp2l61mqrvqe/v1
https://doi.org/10.17504/protocols.io.bp2l61mqrvqe/v1
This protocol is used for the rapid generation of 3’ transcriptomic-NGS-ready- single-cell-libraries from pools of cells. Note: Research reported in this publication was supported by the National Institute Of Mental Health of the National Institute
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::21dcef3e8b8507f9c566737960e57d2c
https://doi.org/10.17504/protocols.io.dm6gpwd8jlzp/v2
https://doi.org/10.17504/protocols.io.dm6gpwd8jlzp/v2
This SOP provides instruction to prepare Internal Solution which is used for patch clamp electrophysiology, modified for cleanliness and mRNA capture and sustainability. Note: Research reported in this publication was supported by the National Instit
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::8255ae685307c227579fe28630295ef4
https://doi.org/10.17504/protocols.io.b8hirt4e
https://doi.org/10.17504/protocols.io.b8hirt4e
This protocol describes how to isolate tissue from various ROI’s of the mouse brain followed by preparation of a highly concentrated single cell suspension for transcriptomic profiling on 10x Genomics platform. Note: Research reported in this publi
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::c9601336e9373999ad93f786cd3c8837
https://doi.org/10.17504/protocols.io.b7y7rpzn
https://doi.org/10.17504/protocols.io.b7y7rpzn
Isolation of nuclei from frozen adult human brain tissue or thawed and microdissected brain tissue sections for FPCR and/or RNA-seq analysis. Note: Research reported in this publication was supported by the National Institute Of Mental Health of the
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::401d7c131577738ab205c0bbddb8e86b
https://doi.org/10.17504/protocols.io.ewov149p7vr2/v2
https://doi.org/10.17504/protocols.io.ewov149p7vr2/v2
This protocol is used to generate accurate digital representations of neuron morphologies from a variety of brain regions and species. Each reconstruction captures the positions and thicknesses of the soma, dendrites and axon of a biocytin-filled cel
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::0f2e0102b8dfb61d830a569b235db14f
https://doi.org/10.17504/protocols.io.36wgq5683gk5/v3
https://doi.org/10.17504/protocols.io.36wgq5683gk5/v3
This protocol is a detailed version of the Allen Institute's Patch-Seq Recording and Extraction protocol, which describes the process to obtain electrophysiological recordings and cellular contents from neurons in postnatal mouse and/or human brain s
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::088d8091d403dec9b03c0fd39c4a0427
https://doi.org/10.17504/protocols.io.bw6gphbw
https://doi.org/10.17504/protocols.io.bw6gphbw