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pro vyhledávání: '"Allan. Witkowski"'
Publikováno v:
Analytical Chemistry. 67:1301-1306
A biotinylated β-galactosidase conjugate prepared by the posttanslational modification of a recombinant fusion protein was used in the development of a heterogeneous binding assay for biotin. This conjugate was biotinylated at a predetermined locati
Publikováno v:
Mikrochimica Acta. 121:63-72
Potentiometric enzyme electrodes for urea were prepared by the covalent attachment of urease to a functionalized polypyrrole film. In particular, a polypyrrole film was first deposited on the surface of a glassy-carbon electrode and then functionaliz
Publikováno v:
Analytical Chemistry. 66:1837-1840
Biotinylated recombinant aequorin was used in the development of a homogeneous bioluminescence assay for biotin. It was demonstrated that the Ca 2+ -triggered luminescent reaction of biotinylated aequorin can be inhibited by the presence of avidin, a
Publikováno v:
Electroanalysis. 10:58-60
Poly(biotinyl-tyramide) films were created by the electrochemical oxidation of biotinyl-tyramide. Electropolymerization in basic solutions such as NaOH/methanol or aqueous NaOH led to adherent poly(biotinyl) films on both glassy-carbon (GC) and indiu
Publikováno v:
17th AIAA Aerodynamic Decelerator Systems Technology Conference and Seminar.
Final design development and structural qualification testing of the planetary entry parachute system for the Mars Exploration Rover (MER) mission was performed using the 80-by-120-Foot Wind Tunnel at NASA Ames Research Center. Full-scale parachutes
Publisher Summary This chapter describes competitive binding assays for biotin that use the bioluminescent protein aequorin as the label. Heterogeneous and homogeneous competitive binding assays using aequorin bioluminescence have been developed for
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::03be4e5a66517161e170847470ec8e5b
https://doi.org/10.1016/s0076-6879(97)79033-5
https://doi.org/10.1016/s0076-6879(97)79033-5
Publikováno v:
Interfacial Design and Chemical Sensing ISBN: 9780841229310
Interfacial Design and Chemical Sensing
Interfacial Design and Chemical Sensing
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::0f646396abfca7a61e0b0e6891a613a2
https://doi.org/10.1021/bk-1994-0561.ch025
https://doi.org/10.1021/bk-1994-0561.ch025
Publikováno v:
Analytical chemistry. 65(9)
Traditional chemical means of preparing enzyme-ligand conjugates for use in enzyme-linked immunosorbent assays (ELISAs) lead to the production of multisubstituted enzyme-ligand conjugates with a high degree of variability in the site of ligand attach
Autor:
Anna Brajter-Toth, Allan. Witkowski
Publikováno v:
Analytical chemistry. 64(6)
Studies have been conducted to further characterize overoxidized polypyrrole films grown on carbon electrodes. Differences in apparent diffusion coefficients for various probes are interpreted via a model based on both hydrophobic and electrostatic i
Publikováno v:
Analytical chemistry. 63(6)
Polypyrrole (PP) films were electropolymerized on glassy carbon (GC) and rough pyrolytic graphite (RPG) electrodes and treated by overoxidation in sodium hydroxide and phosphate buffer. The effects of treatment on film morphology were investigated, i