Novel Inhibitory Function of the Rhizomucor miehei Lipase Propeptide and Three-Dimensional Structures of Its Complexes with the Enzyme

Autor: Olga V. Moroz, Elena Blagova, Verena Reiser, Rakhi Saikia, Sohel Dalal, Christian Isak Jørgensen, Vikram K. Bhatia, Lone Baunsgaard, Birgitte Andersen, Allan Svendsen, Keith S. Wilson

Publikováno v: ACS Omega, Vol 4, Iss 6, Pp 9964-9975 (2019)

Externí odkaz: https://doaj.org/article/89c795affba548c9a855eee88d9d671e

A computational methodology to screen activities of enzyme variants.

Autor: Martin R Hediger, Luca De Vico, Allan Svendsen, Werner Besenmatter, Jan H Jensen

Publikováno v: PLoS ONE, Vol 7, Iss 12, p e49849 (2012)

We present a fast computational method to efficiently screen enzyme activity. In the presented method, the effect of mutations on the barrier height of an enzyme-catalysed reaction can be computed within 24 hours on roughly 10 processors. The methodo

Externí odkaz: https://doaj.org/article/40704339941b41f99d0c1933e5fc335c

Direct observation of Thermomyces lanuginosus lipase diffusional states by Single Particle Tracking and their remodeling by mutations and inhibition

Autor: Allan Svendsen, Johannes Thomsen, Søren S.-R. Bohr, Nikos S. Hatzakis, Henrik Dahl Pinholt, Sune M. Christensen, Amalie S. Kallenbach, Lars Iversen, Philip M. Lund

Publikováno v: Bohr, S S-R, Lund, P M, Kallenbach, A S, Pinholt, H, Thomsen, J, Iversen, L, Svendsen, A, Christensen, S M & Hatzakis, N S 2019, ' Direct observation of Thermomyces lanuginosus lipase diffusional states by Single Particle Tracking and their remodeling by mutations and inhibition ', Scientific Reports, vol. 9, 16169 . https://doi.org/10.1038/s41598-019-52539-1
Scientific Reports
Scientific Reports, Vol 9, Iss 1, Pp 1-11 (2019)

Lipases are interfacially activated enzymes that catalyze the hydrolysis of ester bonds and constitute prime candidates for industrial and biotechnological applications ranging from detergent industry, to chiral organic synthesis. As a result, there

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::2a92db2807417714a506d286593b8a73
https://doi.org/10.1038/s41598-019-52539-1

Molecular Modeling Investigation of the Interaction between Humicola insolens Cutinase and SDS Surfactant Suggests a Mechanism for Enzyme Inactivation

Autor: Leonardo De Maria, Andrea Coletta, Allan Svendsen, Xavier Periole, Mikkel Vestergaard, Lisbeth Ravnkilde Kjølbye, Anne Kjær Laustsen, Birgit Schiøtt

Publikováno v: Kjølbye, L R, Laustsen, A, Vestergaard, M, Periole, X, De Maria, L, Svendsen, A, Coletta, A & Schiøtt, B 2019, ' Molecular Modeling Investigation of the Interaction between Humicola insolens Cutinase and SDS Surfactant Suggests a Mechanism for Enzyme Inactivation ' Journal of Chemical Information and Modeling . https://doi.org/10.1021/acs.jcim.8b00857
Kjølbye, L R, Laustsen, A K, Vestergaard, M, Periole, X, De Maria, L, Svendsen, A, Coletta, A & Schiøtt, B 2019, ' Molecular Modeling Investigation of the Interaction between Humicola insolens Cutinase and SDS Surfactant Suggests a Mechanism for Enzyme Inactivation ', Journal of Chemical Information and Modeling . https://doi.org/10.1021/acs.jcim.8b00857
Kjolbye, L R, Laustsen, A, Vestergaard, M, Periole, X, De Maria, L, Svendsen, A, Coletta, A & Schiott, B 2019, ' Molecular Modeling Investigation of the Interaction between Humicola insolens Cutinase and SDS Surfactant Suggests a Mechanism for Enzyme Inactivation ', Journal of Chemical Information and Modeling, vol. 59, no. 5, pp. 1977-1987 . https://doi.org/10.1021/acs.jcim.8b00857

One of the largest commercial applications of enzymes and surfactants is as main components in modern detergents. The high concentration of surfactant compounds usually present in detergents can, however, negatively affect the enzymatic activity. To

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::13941a3a856b06799500f7fc7e6b130c
https://pure.au.dk/portal/da/publications/molecular-modeling-investigation-of-the-interaction-between-humicola-insolens-cutinase-and-sds-surfactant-suggests-a-mechanism-for-enzyme-inactivation(0feb2f74-c991-411b-85cc-77652d005286).html

Thermomyces lanuginosus lipase-catalyzed hydrolysis of the lipid cubic liquid crystalline nanoparticles

Autor: Justas Barauskas, Allan Svendsen, Hanna I. Anderberg, Tommy Nylander

Publikováno v: Colloids and Surfaces B: Biointerfaces. 137:50-59

In this study well-ordered glycerol monooleate (GMO)-based cubic liquid crystalline nanoparticles (LCNPs) have been used as substrates for Thermomyces lanuginosus lipase in order to establish the relation between the catalytic activity, measured by p

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::2e929867ab54f5f3b692181851ccbaa3
https://doi.org/10.1016/j.colsurfb.2015.04.052