Zobrazeno 1 - 10
of 77
pro vyhledávání: '"Allan L. Bieber"'
Publikováno v:
Molecular Reproduction and Development. 78:450-462
Previously we have shown that extracts from Xenopus egg jelly (egg water) increase the passage of sperm through a porous membrane in a dose-dependent manner. Although this assay has shown that sperm accumulation occurs only in the presence of an egg
Autor:
Ericka Mclaughlin, Douglas E. Chandler, Sumera Naqvi, Nathan Tholl, Allan L. Bieber, Serenity Boyles
Publikováno v:
The Biological Bulletin. 220:174-185
The motility of Xenopus sperm is initiated by the osmotic shock experienced when these cells are ejacu- lated into low-salinity pond water. Motility is brief and is required for the sperm to penetrate the jelly layers and fertilize the egg. In this s
Autor:
Amy Miao, Allan L. Bieber, Shaun Willis, Lindsey A. Burnett, Douglas E. Chandler, Hitoshi Sugiyama, Tatsuo Akema, John H. Olson, Xueyu Xiang
Publikováno v:
Molecular Reproduction and Development. 76:527-536
Allurin, a sperm chemoattractant isolated from Xenopus laevis egg jelly, can be purified in one step from an extract of diffusible jelly proteins (“egg water”) using a FPLC or HPLC anion exchange column and a multi-step NaCl gradient. Allurin hom
Publikováno v:
The International Journal of Developmental Biology. 52:489-501
Crisp proteins appear to play multiple roles in the life history of sperm. One of these roles is to act as a sperm chemoattractant. Allurin, a 21 kDa Crisp protein rapidly released from the egg jelly of at least two frogs, X. laevis and X. tropicalis
Autor:
Allan L. Bieber, Kemmons A. Tubbs, Randall W. Nelson, Eric E. Niederkofler, Urban A. Kiernan, Dobrin Nedelkov
Publikováno v:
Analytical Chemistry. 78:3271-3276
This report addresses the need for additional assays for human resistin (hRES) by developing a rational progression of the mass spectrometric immunoassay to incorporate recombinant proteins. The recombinant-based hRES mass spectrometric immunoassay (
Autor:
Dobrin Nedelkov, Urban A. Kiernan, Allan L. Bieber, Randall W. Nelson, Eric E. Niederkofler, Kemmons A. Tubbs
Publikováno v:
PROTEOMICS. 5:5002-5007
A high-throughput affinity capture and reduction approach was developed for phenotype and post-translational modification analysis of a complexed globular protein, haptoglobin (Hp), directly from human plasma. Hp was selectively retrieved utilizing a
Publikováno v:
Developmental Biology. 275:343-355
Recently, we cloned and sequenced the cDNA of allurin, a sperm chemoattractant isolated from the jelly of Xenopus laevis eggs [Proc. Natl. Acad. Sci. U.S.A. 78 (2001) 11205]. In this report, we demonstrate that allurin mRNA is expressed almost exclus
Publikováno v:
Molecular & Cellular Proteomics. 3:266-272
An alkaline phosphatase-bioreactive probe, in which the enzyme is covalently bound to the mass spectrometry target, has been developed for studies of phosphoproteins. The bioreactive probe was used in combination with affinity capture and matrix-assi
Publikováno v:
Molecular Reproduction and Development. 70:344-360
Allurin, a 21 kD protein from Xenopus laevis egg jelly, has been demonstrated to attract sperm by video microscopy and by quantitative chemotaxis chamber assays. Here, we use immunocytochemistry to demonstrate that this sperm chemoattractant is locat
Autor:
Arcadius V. Krivoshein, Kemmons A. Tubbs, Randall W. Nelson, Allan L. Bieber, Ronald J. Lukas
Publikováno v:
Analytical Biochemistry. 301:175-188
Studies were conducted to optimize matrix-assisted laser desorption/ionization, time-of-flight mass spectrometry (MALDI TOF MS) in analyzing the composition of nicotinic acetylcholine receptors (nAChR) from Torpedo californica electric tissue in thei