Zobrazeno 1 - 10
of 206
pro vyhledávání: '"Allan C Spradling"'
Publikováno v:
eLife, Vol 12 (2023)
Highly potent animal stem cells either self renew or launch complex differentiation programs, using mechanisms that are only partly understood. Drosophila female germline stem cells (GSCs) perpetuate without change over evolutionary time and generate
Externí odkaz:
https://doaj.org/article/9157b37e1c454bf6bae501445ff206fc
Publikováno v:
eLife, Vol 11 (2022)
Recent studies show that pre-follicular mouse oogenesis takes place in germline cysts, highly conserved groups of oogonial cells connected by intercellular bridges that develop as nurse cells as well as an oocyte. Long studied in Drosophila and insec
Externí odkaz:
https://doaj.org/article/fa3dc0cac3b34ceea2dc3305eb54a784
Publikováno v:
eLife, Vol 9 (2020)
Polycomb silencing represses gene expression and provides a molecular memory of chromatin state that is essential for animal development. We show that Drosophila female germline stem cells (GSCs) provide a powerful system for studying Polycomb silenc
Externí odkaz:
https://doaj.org/article/b121fcefa7fc4809b01606c3ffbe0089
Autor:
Chenhui Wang, Allan C Spradling
Publikováno v:
eLife, Vol 9 (2020)
Adult Drosophila Malpighian tubules have low rates of cell turnover but are vulnerable to damage caused by stones, like their mammalian counterparts, kidneys. We show that Drosophilarenal stem cells (RSCs) in the ureter and lower tubules comprise a u
Externí odkaz:
https://doaj.org/article/698225ea7338441fb3417b8f6fa414dd
Autor:
Oguz Kanca, Jonathan Zirin, Jorge Garcia-Marques, Shannon Marie Knight, Donghui Yang-Zhou, Gabriel Amador, Hyunglok Chung, Zhongyuan Zuo, Liwen Ma, Yuchun He, Wen-Wen Lin, Ying Fang, Ming Ge, Shinya Yamamoto, Karen L Schulze, Yanhui Hu, Allan C Spradling, Stephanie E Mohr, Norbert Perrimon, Hugo J Bellen
Publikováno v:
eLife, Vol 8 (2019)
We previously reported a CRISPR-mediated knock-in strategy into introns of Drosophila genes, generating an attP-FRT-SA-T2A-GAL4-polyA-3XP3-EGFP-FRT-attP transgenic library for multiple uses (Lee et al., 2018a). The method relied on double stranded DN
Externí odkaz:
https://doaj.org/article/fbf9b18e540242aa9bc3f668e020beb1
Publikováno v:
eLife, Vol 8 (2019)
Human oocytes frequently generate aneuploid embryos that subsequently miscarry. In contrast, Drosophila oocytes from outbred laboratory stocks develop fully regardless of maternal age. Since mature Drosophila oocytes are not extensively stored in the
Externí odkaz:
https://doaj.org/article/ff381f4e727040ad8bf44c4b17538dbb
Autor:
Pei-Tseng Lee, Jonathan Zirin, Oguz Kanca, Wen-Wen Lin, Karen L Schulze, David Li-Kroeger, Rong Tao, Colby Devereaux, Yanhui Hu, Verena Chung, Ying Fang, Yuchun He, Hongling Pan, Ming Ge, Zhongyuan Zuo, Benjamin E Housden, Stephanie E Mohr, Shinya Yamamoto, Robert W Levis, Allan C Spradling, Norbert Perrimon, Hugo J Bellen
Publikováno v:
eLife, Vol 7 (2018)
We generated a library of ~1000 Drosophila stocks in which we inserted a construct in the intron of genes allowing expression of GAL4 under control of endogenous promoters while arresting transcription with a polyadenylation signal 3’ of the GAL4.
Externí odkaz:
https://doaj.org/article/df67f9797a394b558a57f36f0b13882f
Publikováno v:
PLoS ONE, Vol 11, Iss 3, p e0151251 (2016)
Tissue integrity and homeostasis often rely on the proliferation of stem cells or differentiated cells to replace lost, aged, or damaged cells. Recently, we described an alternative source of cell replacement- the expansion of resident, non-dividing
Externí odkaz:
https://doaj.org/article/caed38ad3a5c408bbc7874b77b4f12ab
Autor:
Sonal Nagarkar-Jaiswal, Steven Z DeLuca, Pei-Tseng Lee, Wen-Wen Lin, Hongling Pan, Zhongyuan Zuo, Jiangxing Lv, Allan C Spradling, Hugo J Bellen
Publikováno v:
eLife, Vol 4 (2015)
Previously, we described a large collection of Minos-Mediated Integration Cassettes (MiMICs) that contain two phiC31 recombinase target sites and allow the generation of a new exon that encodes a protein tag when the MiMIC is inserted in a codon intr
Externí odkaz:
https://doaj.org/article/d0b3068e8f7c46cea2d2e55da0a8619c
Autor:
Sonal Nagarkar-Jaiswal, Pei-Tseng Lee, Megan E Campbell, Kuchuan Chen, Stephanie Anguiano-Zarate, Manuel Cantu Gutierrez, Theodore Busby, Wen-Wen Lin, Yuchun He, Karen L Schulze, Benjamin W Booth, Martha Evans-Holm, Koen JT Venken, Robert W Levis, Allan C Spradling, Roger A Hoskins, Hugo J Bellen
Publikováno v:
eLife, Vol 4 (2015)
Here, we document a collection of ∼7434 MiMIC (Minos Mediated Integration Cassette) insertions of which 2854 are inserted in coding introns. They allowed us to create a library of 400 GFP-tagged genes. We show that 72% of internally tagged proteins
Externí odkaz:
https://doaj.org/article/a360211c69524b5c898cb3e9e9faee55