Výsledky vyhledávání - "Alizée Vergès"

Neutral genetic drift-based engineering of a sucrose-utilizing enzyme toward glycodiversification

Autor: David Daudé, Alizée Vergès, Magali Remaud-Simeon, Stéphane Emond, Isabelle André, Emmanuelle Cambon, Samuel Tranier

Publikováno v: 'ACS Catalysis ', vol: 9, pages: 1241-1252 (2019)
ACS Catalysis
ACS Catalysis, American Chemical Society, 2019, 9 (2), pp.1241-1252. ⟨10.1021/acscatal.8b03609⟩
ACS Catalysis, 2019, 9 (2), pp.1241-1252. ⟨10.1021/acscatal.8b03609⟩

Neutral drift (also called purifying selection) is an attractive approach to generate polymorphic variant libraries for enzyme engineering. Here, we have applied this strategy to modify the substrate specificity of a transglucosylase. Our model enzym

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::e58a221d0a8f5cb385cc3054d42e6dbb
https://epn-library.esrf.fr/flora/jsp/index_view_direct_anonymous.jsp?record=doc:PUB_ESRF:51294

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Novel product specificity toward erlose and panose exhibited by multisite engineered mutants of amylosucrase

Autor: Magali Remaud-Simeon, Sophie Barbe, Isabelle André, Emmanuelle Cambon, Claire Moulis, Alizée Vergès

Publikováno v: Protein Science. 26:566-577

A computer-aided engineering approach recently enabled to deeply reshape the active site of N. polysaccharea amylosucrase for recognition of non-natural acceptor substrates. Libraries of variants were constructed and screened on sucrose allowing the

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_________::414556f70fa34191af236c615ef184a9
https://doi.org/10.1002/pro.3106

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Engineering of anp efficient mutant of Neisseria polysaccharea amylosucrase for the synthesis of controlled size maltooligosaccharides

Autor: Sophie Barbe, Alizée Vergès, Emmanuelle Cambon, Isabelle André, Magali Remaud-Simeon, Samuel Tranier, Claire Moulis

Publikováno v: Carbohydrate Polymers
Carbohydrate Polymers, Elsevier, 2017, 173, pp.403-411. ⟨10.1016/j.carbpol.2017.06.011⟩
Carbohydrate Polymers, 2017, 173, pp.403-411. ⟨10.1016/j.carbpol.2017.06.011⟩

Amylosucrase from Neisseria polysaccharea naturally catalyzes the synthesis of α-1,4 glucans from sucrose. The product profile is quite polydisperse, ranging from soluble chains called maltooligosaccharides to high-molecular weight insoluble amylose

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::07ad3fd3b75c845a5ce6069e40746db3
https://hal.archives-ouvertes.fr/hal-01602744

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Computer-Aided Engineering of a Transglycosylase for the Glucosylation of an Unnatural Disaccharide of Relevance for Bacterial Antigen Synthesis

Autor: Yann Le Guen, Isabelle André, Emmanuelle Cambon, Sophie Barbe, Magali Remaud-Simeon, Laurence A. Mulard, Stéphane Salamone, Claire Moulis, Alizée Vergès

Publikováno v: ACS Catalysis
ACS Catalysis, 2015, 5 (2), pp.1186-1198. ⟨10.1021/cs501288r⟩
ACS Catalysis, American Chemical Society, 2015, 5 (2), pp.1186-1198. ⟨10.1021/cs501288r⟩

International audience; The exploration of chemo-enzymatic routes to complex carbohydrates has been hampered by the lack of appropriate enzymatic tools having the substrate specificity for new reactions. Here, we used a computer-aided design framewor

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::aeffb479c26cb836b193805bb80eaad5
https://hal-pasteur.archives-ouvertes.fr/pasteur-02043360

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