A multiplexed, paired-pooled droplet digital PCR assay for detection of SARS-CoV-2 in saliva

Autor: Kaitlyn Wagner, Phil Fox, Elizabeth Gordon, Westen Hahn, Kenzie Olsen, Alex Markham, Dylan Buglewicz, Platon Selemenakis, Avery Lessard, Daniella Goldstein, Alissa Threatt, Luke Davis, Jake Miller-Dawson, Halie Stockett, Hailey Sanders, Kristin Rugh, Houston Turner, Michelle Remias, Maggie Williams, Jorge Chavez, Gabriel Galindo, Charlotte Cialek, Amanda Koch, Alex Fout, Bailey Fosdick, Bettina Broeckling, Mark D. Zabel

Publikováno v: Scientific Reports, Vol 13, Iss 1, Pp 1-17 (2023)

Abstract In response to the SARS-CoV-2 pandemic, we developed a multiplexed, paired-pool droplet digital PCR (MP4) screening assay. Key features of our assay are the use of minimally processed saliva, 8-sample paired pools, and reverse-transcription

Externí odkaz: https://doaj.org/article/1ccf3eb2288a4097b49c733fdd635a24

A multiplexed, paired-pooled droplet digital PCR assay for detection of SARS-CoV-2 in saliva.

Autor: Wagner, Kaitlyn^1,2 (AUTHOR), Fox, Phil^1,2 (AUTHOR), Gordon, Elizabeth^1,2 (AUTHOR), Hahn, Westen^1,2 (AUTHOR), Olsen, Kenzie^1,2 (AUTHOR), Markham, Alex^1,2 (AUTHOR), Buglewicz, Dylan^1,2 (AUTHOR), Selemenakis, Platon^1,2 (AUTHOR), Lessard, Avery^1,2 (AUTHOR), Goldstein, Daniella^1,2 (AUTHOR), Threatt, Alissa^1,2 (AUTHOR), Davis, Luke^1,2 (AUTHOR), Miller-Dawson, Jake^1,2 (AUTHOR), Stockett, Halie^1,2 (AUTHOR), Rugh, Kristin^1,2 (AUTHOR), Turner, Houston^1,2 (AUTHOR), Remias, Michelle^1,2 (AUTHOR), Williams, Maggie^1,2 (AUTHOR), Chavez, Jorge^1,2 (AUTHOR), Galindo, Gabriel^1,2 (AUTHOR)

Publikováno v: Scientific Reports. 2/22/2023, Vol. 13 Issue 1, p1-17. 17p.