The performance of human cytomegalovirus digital PCR reference measurement procedure in seven external quality assessment schemes over four years

Autor: Denise M. O'Sullivan, Alexandra Bogožalec Košir, Alison S. Devonshire, Maria Karczmarczyk, Fran Van Heuverswyn, Jim F. Huggett, Annabell Plauth, Gerwyn M. Jones, Heinz Zeichhardt, Jörg Neukammer, Hans-Peter Grunert, Martin Kammel, Jannika Neeb, Carole A. Foy, Andreas Kummrow, Philippe Corbisier, Jernej Pavšič, Heinz Schimmel, Mojca Milavec

A candidate digital PCR (dPCR)-based reference measurement procedure for quantification of human cytomegalovirus (hCMV) was evaluated in 10 viral load comparison schemes (seven external quality assessment (EQA) and three additional training schemes)

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::2eb0a92008a28eb8a23aa5dce060e0dc
http://hdl.handle.net/1942/37662

International Interlaboratory Digital PCR Study Demonstrating High Reproducibility for the Measurement of a Rare Sequence Variant

Autor: Julia Beck, Simon Cowen, Anne Pradines, Lao H. Saal, Elliot Stieglitz, Lisa Davis, Silvia Galbiati, Alexandra Pender, Tim Forshew, Filip Janku, Roger Lacave, Svilen Tzonev, Charlotte Proudhon, Valérie Combaret, Jim F. Huggett, Gerwyn M. Jones, Ana Fernandez-Gonzalez, Justin Lee, Bryan C. Ulrich, Alexandra S. Whale, Helen C. Parkes, Nicholas Redshaw, Carole A. Foy, Alison S. Devonshire, Frederic Fina, Rikke Fredslund Andersen, Andreas Berger, Vilas Mistry, Leanne Javier, George Karlin-Neumann, John F. Regan, Álvaro González Hernández, Nina Dahl Kjersgaard, Charles A. Haynes

Publikováno v: Whale, A S, Devonshire, A S, Karlin-Neumann, G, Regan, J, Javier, L, Cowen, S, Fernandez-Gonzalez, A, Jones, G M, Redshaw, N, Beck, J, Berger, A W, Combaret, V, Kjersgaard, N D, Davis, L, Fina, F, Forshew, T, Andersen, R F, Galbiati, S, Hernandez, A G, Haynes, C A, Janku, F, Lacave, R, Lee, J, Mistry, V, Pender, A, Pradines, A, Proudhon, C, Saal, L H, Stieglitz, E, Ulrich, B, Foy, C A, Parkes, H, Tzonev, S & Huggett, J F 2017, ' International interlaboratory digital PCR study demonstrating high reproducibility for the measurement of a rare sequence variant ', Analytical Chemistry, vol. 89, no. 3, pp. 1724-1733 . https://doi.org/10.1021/acs.analchem.6b03980

This study tested the claim that digital PCR (dPCR) can offer highly reproducible quantitative measurements in disparate laboratories. Twenty-one laboratories measured four blinded samples containing different quantities of a KRAS fragment encoding G

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::ebc01d24aa5634e6fdf1096bd5afb8ec
https://doi.org/10.1021/acs.analchem.6b03980

Control Materials and Digital PCR Methods for Evaluation of Circulating Cell-Free DNA Extractions from Plasma

Autor: Alexandra S, Whale, Ana, Fernandez-Gonzalez, Alice, Gutteridge, Alison S, Devonshire

Publikováno v: Methods in molecular biology (Clifton, N.J.). 1768

Cell-free DNA is an accessible source of genetic material found naturally in plasma that could be used in many diagnostic applications. Translation of cfDNA analysis methods from research laboratories into the clinic would benefit from controls for m

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=pmid________::ec8248f22a77768575826cb3bc58629b
https://pubmed.ncbi.nlm.nih.gov/29717437

Standardization of Nucleic Acid Tests for Clinical Measurements of Bacteria and Viruses

Autor: Mojca Milavec, Helen C. Parkes, Alison S. Devonshire, Carole A. Foy, Heinz Schimmel, Jana Žel, Isobella Honeyborne, Maria Karczmarczyk, Ion Gutiérrez-Aguirre, Jernej Pavšič, Jim F. Huggett, Heinz Zeichhardt, Timothy D. McHugh

Publikováno v: Journal of Clinical Microbiology. 53:2008-2014

Nucleic acid-based tests for infectious diseases currently used in the clinical laboratory and in point-of-care devices are diverse. Measurement challenges associated with standardization of quantitative viral load testing are discussed in relation t

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::a6f1dcc385c313d70446809f553f6e58
https://doi.org/10.1128/jcm.02136-14

Assessment of Digital PCR as a Primary Reference Measurement Procedure to Support Advances in Precision Medicine

Autor: Valérie Taly, Helen C. Parkes, Kenneth D. Cole, Carole A. Foy, Denise M. O'Sullivan, Simon Cowen, Carla Divieto, Hua-Jun He, Sema Akyürek, Sonia Garrigou, Maria Paola Sassi, Muslum Akgoz, Alexandra S. Whale, Tanja Dreo, Nicholas Redshaw, Alison J Woolford, Sang-Ryoul Park, Young-Kyung Bae, Heidi Goenaga-Infante, Raquel Larios, Alison S. Devonshire, Jernej Pavšič, Liesbet Deprez, Jim F. Huggett, Gerwyn M. Jones, Philippe Corbisier, Claire A Bushell

Publikováno v: Clinical Chemistry
Clinical Chemistry, American Association for Clinical Chemistry, 2018, 64 (9), pp.1296-1307. ⟨10.1373/clinchem.2017.285478⟩

BACKGROUND Genetic testing of tumor tissue and circulating cell-free DNA for somatic variants guides patient treatment of many cancers. Such measurements will be fundamental in the future support of precision medicine. However, there are currently no

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::66cbb1e018d405d39290db05d2f37f31
https://pubmed.ncbi.nlm.nih.gov/29903874