Zobrazeno 1 - 10
of 19
pro vyhledávání: '"Alison R. Gillies"'
Publikováno v:
Biosensors and Bioelectronics. 29:132-139
The peroxisome proliferator-activated receptor gamma (PPARγ or PPARG) belongs to the nuclear receptor superfamily, and is a potential drug target for a variety of diseases. In this work, we constructed a series of bacterial biosensors for the identi
Autor:
Randy J. Zauhar, Nitya Royapet, David W. Wood, Christina Andaya, Alison R. Gillies, Sonia Arora, Izabela Hartman, William J. Welsh
Publikováno v:
Pharmaceutical Research. 26:2247-2258
In this study, two unreported estrogen antagonists were identified using a combination of computational screening and a simple bacterial estrogen sensor.Molecules here presented were initially part of a group obtained from a library of over a half mi
Autor:
David W. Wood, Alison R. Gillies
Publikováno v:
Protein Engineering Handbook, Volume 1 & Volume 2
Publikováno v:
Biotechnology and Bioengineering. 101:229-240
We have combined Invitrogen’s Gateway 1 cloning technology with self-cleaving purification tags togenerate a new system for rapid production of recombinantprotein products. To accomplish this, we engineered ourpreviously reported DI-CM cleaving in
Autor:
Seachol Oak, Lydia M. Contreras, Alison R. Gillies, Judy F. Hsii, Wan-Yi Wu, Michael B. Perl, David W. Wood
Publikováno v:
Biotechnology progress. 26(5)
In this work, our previously reported ΔI-CM and ΔI(G)-CM mutant inteins were rationally re-engineered to be compatible with Invitrogen's Topo® cloning system. The resulting new inteins include the vaccinia virus topoisomerase I DNA recognition seq
Autor:
Gary LeRoy, Iraj Ghazi, Baley A. Fong, David W. Wood, Alison R. Gillies, Lars F. Westblade, Kathleen C. Lee
Publikováno v:
Protein science : a publication of the Protein Society. 19(6)
A self-cleaving elastin-like polypeptide (ELP) tag was used to purify the multisubunit Escherichia coli RNA polymerase (RNAP) via a simple, nonchromatographic method. To accomplish this, the RNAP alpha subunit was tagged with a self-cleaving ELP-inte
Publikováno v:
Methods in Molecular Biology ISBN: 9781588298799
A method has been developed that eliminates the need for complex chromatographic apparatus in the purification of recombinant proteins expressed in Escherichia coli. This method is similar to conventional affinity-tag separations, but the affinity re
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::0ff877bbdd76047462cd45264fc11bf6
https://doi.org/10.1007/978-1-59745-196-3_12
https://doi.org/10.1007/978-1-59745-196-3_12
Publikováno v:
Methods in molecular biology (Clifton, N.J.). 498
A method has been developed that eliminates the need for complex chromatographic apparatus in the purification of recombinant proteins expressed in Escherichia coli. This method is similar to conventional affinity-tag separations, but the affinity re
Publikováno v:
Biotechnology progress. 24(1)
Biomolecular engineering has many applications in the identification of potentially therapeutic compounds. An important class of these compounds is those that bind and modulate the activity of the human nuclear hormone receptors (NHRs). NHRs are typi
Publikováno v:
Biotechnology Progress; Feb2008, Vol. 24 Issue 1, p8-16, 9p