Zobrazeno 1 - 10
of 24
pro vyhledávání: '"Alison M. Skelley"'
Autor:
Curt I, Civin, Tony, Ward, Alison M, Skelley, Khushroo, Gandhi, Zendra, Peilun Lee, Christopher R, Dosier, Joseph L, D'Silva, Yu, Chen, MinJung, Kim, James, Moynihan, Xiaochun, Chen, Lee, Aurich, Sergei, Gulnik, George C, Brittain, Diether J, Recktenwald, Robert H, Austin, James C, Sturm
Publikováno v:
Cytometry. Part A : the journal of the International Society for Analytical Cytology. 89(12)
We previously developed a Deterministic Lateral Displacement (DLD) microfluidic method in silicon to separate cells of various sizes from blood (Davis et al., Proc Natl Acad Sci 2006;103:14779-14784; Huang et al., Science 2004;304:987-990). Here, we
Publikováno v:
Nature methods
Cell fusion has been used for many different purposes, including generation of hybridomas and reprogramming of somatic cells. The fusion step represents the key event in initiation of these procedures. Standard fusion techniques, however, provide poo
Publikováno v:
Analytical Chemistry. 79:8162-8169
A portable microfabricated capillary electrophoresis (CE) instrument is used for the determination of neurologically active biogenic amines, especially tyramine and histamine, in fermented beverages. The target molecules are labeled on their primary
Autor:
Alison M. Skelley, Richard A. Mathies
Publikováno v:
Journal of Chromatography A. 1132:304-309
A novel electrophoretic microchip method for analyzing alpha- and beta-d-glucosamine and their interconversion in solution is presented. d-Glucosamine is labeled with fluorescamine and analyzed by capillary electrophoresis in under 2 min revealing it
Publikováno v:
Analytical Chemistry. 78:7522-7527
In vitro evolution of RNA molecules requires a method for executing many consecutive serial dilutions. To solve this problem, a microfluidic circuit has been fabricated in a three-layer glass-PDMS-glass device. The 400-nL serial dilution circuit cont
Autor:
Andrew D. Aubrey, James R. Scherer, Robin H. C. Ivester, Pascale Ehrenfreund, Frank J. Grunthaner, Richard A. Mathies, Alison M. Skelley, Jeffrey L. Bada, William H. Grover
Publikováno v:
Proceedings of the National Academy of Sciences. 102:1041-1046
The Mars Organic Analyzer (MOA), a microfabricated capillary electrophoresis (CE) instrument for sensitive amino acid biomarker analysis, has been developed and evaluated. The microdevice consists of a four-wafer sandwich combining glass CE separatio
Autor:
Richard A. Mathies, James R. Scherer, Alison M. Skelley, Robert A. Street, Brian M. Paegel, Toshihiro Kamei
Publikováno v:
Journal of Non-Crystalline Solids. :715-719
Hydrogenated amorphous silicon (a-Si:H) pin photodiodes are used as fluorescence sensors for microfluidic analysis devices. A hybrid-integrated sensor consisting of a half-ball lens, a ZnS/YF 3 multilayer optical interference filter with a pinhole, a
Autor:
Alison M. Skelley, Richard A. Mathies
Publikováno v:
Journal of Chromatography A. 1021:191-199
Chiral separations of fluorescamine-labeled amino acids are characterized and optimized on a microfabricated capillary electrophoresis (CE) device. A standard mixture of acidic and neutral amino acids is labeled with fluorescamine in less than 5 min
Autor:
Alison M. Skelley, Richard A. Mathies, Brian M. Paegel, James R. Scherer, Robert A. Street, Toshihiro Kamei
Publikováno v:
Analytical Chemistry. 75:5300-5305
Hydrogenated amorphous silicon (a-Si:H) PIN photodiodes have been developed and characterized as fluorescence detectors for microfluidic analysis devices. A discrete a-Si:H photodiode is first fabricated on a glass substrate and used to detect fluore
Publikováno v:
Sensors and Actuators B: Chemical. 89:315-323
Monolithic elastomer membrane valves and diaphragm pumps suitable for large-scale integration into glass microfluidic analysis devices are fabricated and characterized. Valves and pumps are fabricated by sandwiching an elastomer membrane between etch