Zobrazeno 1 - 10
of 63
pro vyhledávání: '"Alison B. Hickman"'
Publikováno v:
Nature Communications, Vol 14, Iss 1, Pp 1-18 (2023)
Abstract The Hermes DNA transposon is a member of the eukaryotic hAT superfamily, and its transposase forms a ring-shaped tetramer of dimers. Our investigation, combining biochemical, crystallography and cryo-electron microscopy, and in-cell assays,
Externí odkaz:
https://doaj.org/article/7ead86d45c9845d3a5da6f897362bcb0
Publikováno v:
Nature Communications, Vol 11, Iss 1, Pp 1-14 (2020)
PiggyBac is a transposon used in genome engineering that does not leave excision footprints. Here the authors determine the structures of two complexes in which the piggyBac transposase is bound to DNA representing different steps of the transpositio
Externí odkaz:
https://doaj.org/article/73fcb349e4bd4561984b0e66066d5196
Publikováno v:
Nature Communications, Vol 9, Iss 1, Pp 1-14 (2018)
Helitrons are eukaryotic DNA transposons that have profoundly affected genome variation due to their ability to capture and mobilize host genomic fragments. Here the authors provide insight into the mechanism of action of these transposons both in ce
Externí odkaz:
https://doaj.org/article/19f6448310694d889d04bd00fa72fcfc
Autor:
Susu He, Michael Chandler, Alessandro M. Varani, Alison B. Hickman, John P. Dekker, Fred Dyda
Publikováno v:
mBio, Vol 7, Iss 6 (2016)
ABSTRACT The dissemination of resistance among bacteria has been facilitated by the fact that resistance genes are usually located on a diverse and evolving set of transmissible plasmids. However, the mechanisms generating diversity and enabling adap
Externí odkaz:
https://doaj.org/article/e69e1674fffe4b32ab4aab6f6115424c
Publikováno v:
eLife, Vol 9 (2020)
Key to CRISPR-Cas adaptive immunity is maintaining an ongoing record of invading nucleic acids, a process carried out by the Cas1-Cas2 complex that integrates short segments of foreign genetic material (spacers) into the CRISPR locus. It is hypothesi
Externí odkaz:
https://doaj.org/article/41ac509c24284d8ba48eeb0c2864f484
Autor:
Aoshu Zhong, Xiaofang Jiang, Alison B. Hickman, Katherine Klier, Gabriella I. C. Teodoro, Fred Dyda, Michael T. Laub, Gisela Storz
Recombination-promoting nuclease (Rpn) proteins are broadly distributed across bacterial phyla, yet their functions remain unclear. Here we report these proteins are new toxin-antitoxin systems, comprised of genes-within-genes, that combat phage infe
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::5cecaa1960dda595a0207c4703a75733
https://doi.org/10.1101/2023.05.02.539157
https://doi.org/10.1101/2023.05.02.539157
Autor:
Wentian Luo, Alison B Hickman, Pavol Genzor, Rodolfo Ghirlando, Christopher M Furman, Anna Menshikh, Astrid Haase, Fred Dyda, Matthew H Wilson
Publikováno v:
Nucleic Acids Res
Mechanistic regulation of DNA transposon systems in mammalian cells remains poorly understood. Using modeling, biochemical, and cell-based assays, we sought to extend the recent cryoEM structural insight into the piggyBac transpososome to evaluate th
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::9c46cb2b438caec5dd8d6adf2bba5140
https://europepmc.org/articles/PMC9825180/
https://europepmc.org/articles/PMC9825180/
The cut-and-paste Hermes DNA transposase stands out among the transposases that have been biochemically or structurally characterized so far. Many transposases function as dimers, but the Hermes transposase forms a tetramer of dimers to achieve its a
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::1f549dd22f80728bec7a80b1834ebfcc
https://doi.org/10.1101/2022.09.16.508221
https://doi.org/10.1101/2022.09.16.508221
Publikováno v:
The EMBO Journal
Copy‐out/paste‐in transposition is a major bacterial DNA mobility pathway. It contributes significantly to the emergence of antibiotic resistance, often by upregulating expression of downstream genes upon integration. Unlike other transposition p
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::3e4efc5c81c50a0df0ed6d0b1621a470
https://pubmed.ncbi.nlm.nih.gov/33006208
https://pubmed.ncbi.nlm.nih.gov/33006208
Publikováno v:
Nature Communications
Nature Communications, Vol 11, Iss 1, Pp 1-14 (2020)
Nature Communications, Vol 11, Iss 1, Pp 1-14 (2020)
The piggyBac DNA transposon is used widely in genome engineering applications. Unlike other transposons, its excision site can be precisely repaired without leaving footprints and it integrates specifically at TTAA tetranucleotides. We present cryo-E
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::4e36dcf994af8ca680f8dd88b5b52a72
https://pubmed.ncbi.nlm.nih.gov/32651359
https://pubmed.ncbi.nlm.nih.gov/32651359