Lactoyl leucine and isoleucine are bioavailable alternatives for canonical amino acids in cell culture media

Autor: Yannick Rey, Gregor Wille, Aline Zimmer, Corinna Schmidt, Maria Wehsling, Markus Fischer, Dmitry Zabezhinsky, Alisa Schnellbaecher, Maxime Le Mignon

Publikováno v: Biotechnology and Bioengineering

Increasing demands for protein‐based therapeutics such as monoclonal antibodies, fusion proteins, bispecific molecules, and antibody fragments require researchers to constantly find innovative solutions. To increase yields and decrease costs of nex

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::7203bb9f5580bffd59481e32c81389a6
https://doi.org/10.1002/bit.27755

Degradation Products of Tryptophan in Cell Culture Media: Contribution to Color and Toxicity

Autor: Aline Zimmer, Anton Lindig, Alisa Schnellbaecher, Brit Pardon, Maxime Le Mignon, Tim Hofmann, Stephanie Bellmaine

Publikováno v: International Journal of Molecular Sciences, Vol 22, Iss 6221, p 6221 (2021)
International Journal of Molecular Sciences
Volume 22
Issue 12

Biomanufacturing processes may be optimized by storing cell culture media at room temperature, but this is currently limited by their instability and change in color upon long-term storage. This study demonstrates that one of the critical contributin

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::988111a36ed64a0bf4642fa9864ed1b7
https://www.mdpi.com/1422-0067/22/12/6221

Impact of S-sulfocysteine on fragments and trisulfide bond linkages in monoclonal antibodies

Autor: Sandra Maier, Susanne Bohl, Maria Wehsling, Anne Zeck, Ronja Seibel, Alisa Schnellbaecher, Aline Zimmer

Publikováno v: mAbs. 9:889-897

The quality of recombinant proteins such as monoclonal antibodies produced using Chinese hamster ovary cell-based mammalian systems is dependent on many factors, including cell line, process and cell culture media. Due to these factors, the generated

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::ce697066f477b9009adee1b2aa28c063
https://doi.org/10.1080/19420862.2017.1333212

Improvement and simplification of fed-batch bioprocesses with a highly soluble phosphotyrosine sodium salt

Autor: Aline Zimmer, Joerg von Hagen, Maria Wehsling, Ronja Mueller, Alisa Schnellbaecher

Publikováno v: Journal of biotechnology. 186

Fed-batch culture bioprocesses are currently used predominantly for the production of recombinant proteins, especially monoclonal antibodies. In these cultures, concentrated feeds are added during cultivation to prevent nutrient depletion, thus exten

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::09b6d41d5d556f7a77773c17d2ec7267
https://pubmed.ncbi.nlm.nih.gov/25014403