Zobrazeno 1 - 10
of 17
pro vyhledávání: '"Alina Goldstein"'
Publikováno v:
eLife, Vol 10 (2021)
In this study, we analyzed intracellular functions and motile properties of neck-linker (NL) variants of the bi-directional S. cerevisiae kinesin-5 motor, Cin8. We also examined – by modeling – the configuration of H-bonds during NL docking. Decr
Externí odkaz:
https://doaj.org/article/9e18f7c697594ea6acf4979948104de7
Autor:
Himanshu, Pandey, Tatiana, Zvagelsky, Mary, Popov, Mayan, Sadan, Neta, Yanir, Alina, Goldstein-Levitin, Nurit, Siegler, Shira, Hershfinkel, Yahel, Abraham, Roy, Abraham, Levi A, Gheber, Larisa, Gheber
Publikováno v:
Journal of visualized experiments : JoVE. (180)
The mitotic bipolar kinesin-5 motors perform essential functions in spindle dynamics. These motors exhibit a homo-tetrameric structure with two pairs of catalytic motor domains, located at opposite ends of the active complex. This unique architecture
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=pmid________::c5a32107818f87709617d0a670fbc6ba
https://pubmed.ncbi.nlm.nih.gov/35679598
https://pubmed.ncbi.nlm.nih.gov/35679598
Publikováno v:
eLife
eLife, Vol 10 (2021)
eLife, Vol 10 (2021)
In this study, we analyzed intracellular functions and motile properties of neck-linker (NL) variants of the bi-directional S. cerevisiae kinesin-5 motor, Cin8. We also examined – by modeling – the configuration of H-bonds during NL docking. Decr
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::59eff163dffd8381b3f65799b002131e
https://doi.org/10.7554/elife.71036
https://doi.org/10.7554/elife.71036
Publikováno v:
International Journal of Molecular Sciences
International Journal of Molecular Sciences, Vol 22, Iss 6420, p 6420 (2021)
International Journal of Molecular Sciences, Vol 22, Iss 6420, p 6420 (2021)
Bipolar kinesin-5 motor proteins perform multiple intracellular functions, mainly during mitotic cell division. Their specialized structural characteristics enable these motors to perform their essential functions by crosslinking and sliding apart an
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::3868ff55da7be989d18ed551c4e96720
http://europepmc.org/articles/PMC8232732
http://europepmc.org/articles/PMC8232732
Publikováno v:
International Journal of Biological Sciences
Cdk1 has been found to phosphorylate the majority of its substrates in disordered regions, but some substrates maintain precise phosphosite positions over billions of years. Here, we examined the phosphoregulation of the kinesin-5, Cin8, using synthe
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::6b3012cbaffcd280f58813799c5f0f43
https://doi.org/10.7150/ijbs.30543
https://doi.org/10.7150/ijbs.30543
The role of the neck-linker (NL) element in regulating the functions of bi-directional kinesins is unknown. We report that replacing the NL of the bi-directional kinesin-5 Cin8 with sequences from plus-end directed kinesins produces non-functional Ci
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::67dd11770f093e5c2e2593e72cc0ca45
https://doi.org/10.1101/2020.05.27.118430
https://doi.org/10.1101/2020.05.27.118430
Autor:
Jawdat Al-Bassam, Erwin Frey, Emanuel Reithmann, Himanshu Pandey, Alina Goldstein-Levitin, Larisa Gheber
Publikováno v:
Science Advances
Directionality switching of the mitotic kinesin-5 Cin8 motor is caused by an asymmetric response of its active movement to drag.
Directed active motion of motor proteins is a vital process in virtually all eukaryotic cells. Nearly a decade ago,
Directed active motion of motor proteins is a vital process in virtually all eukaryotic cells. Nearly a decade ago,
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::829e7502f136f2787f1e4cddcbbf0724
https://doi.org/10.1101/2020.01.24.918714
https://doi.org/10.1101/2020.01.24.918714
Autor:
Alina Goldstein, Mardo Kõivomägi, Larisa Gheber, Darya Goldman, Ervin Valk, Haim Judah, Nurit Siegler, Mart Loog
Publikováno v:
Cellular and Molecular Life Sciences
The bipolar kinesin-5 motors perform essential functions in mitotic spindle dynamics. We previously demonstrated that phosphorylation of at least one of the Cdk1 sites in the catalytic domain of the Saccharomyces cerevisiae kinesin-5 Cin8 (S277, T285
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::cc28d8283c27bafed8e913c9e403adfe
https://doi.org/10.1007/s00018-017-2523-z
https://doi.org/10.1007/s00018-017-2523-z
Cdk1 has been found to phosphorylate the majority of its substrates in disordered regions. These phosphorylation sites do not appear to require precise positioning for their function. The mitotic kinesin-5 Cin8 was shown to be phosphoregulated at thr
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::c5d063853b2a3cfded47a1bed8138ab4
https://doi.org/10.1101/312637
https://doi.org/10.1101/312637