Zobrazeno 1 - 5
of 5
pro vyhledávání: '"Alicia V. Lis"'
Autor:
Alicia V. Lis, Konstantin Schneider, Jost Weber, Jay D. Keasling, Michael Krogh Jensen, Tobias Klein
Publikováno v:
Microbial Cell Factories, Vol 18, Iss 1, Pp 1-11 (2019)
Abstract Background The physiological characterization of microorganisms provides valuable information for bioprocess development. Chemostat cultivations are a powerful tool for this purpose, as they allow defined changes to one single parameter at a
Externí odkaz:
https://doaj.org/article/e3d169fe6f12459a932c1ae11e4c5183
Autor:
Alicia V. Lis, Lasse Ebdrup Pedersen, Michael Krogh Jensen, Tadas Jakočiūnas, Jay D. Keasling
Publikováno v:
Jakočiūnas, T, Pedersen, L E, Lis, A V, Jensen, M K & Keasling, J D 2018, ' CasPER, a method for directed evolution in genomic contexts using mutagenesis and CRISPR/Cas9 ', Metabolic Engineering, vol. 48, pp. 288-296 . https://doi.org/10.1016/j.ymben.2018.07.001
Here we describe a method for robust directed evolution using mutagenesis of large sequence spaces in their genomic contexts. The method employs error-prone PCR and Cas9-mediated genome integration of mutant libraries of large-sized donor variants in
Autor:
Tobias Klein, Jay D. Keasling, Konstantin Schneider, Alicia V. Lis, Jost Weber, Michael Krogh Jensen
Publikováno v:
Microbial cell factories, vol 18, iss 1
Microbial Cell Factories
Microbial Cell Factories, Vol 18, Iss 1, Pp 1-11 (2019)
Lis, A V, Schneider, K, Weber, J, Keasling, J D, Jensen, M K & Klein, T 2019, ' Exploring small-scale chemostats to scale up microbial processes: 3-hydroxypropionic acid production in S. cerevisiae ', Microbial Cell Factories, vol. 18, no. 1, 50 . https://doi.org/10.1186/s12934-019-1101-5
Microbial Cell Factories
Microbial Cell Factories, Vol 18, Iss 1, Pp 1-11 (2019)
Lis, A V, Schneider, K, Weber, J, Keasling, J D, Jensen, M K & Klein, T 2019, ' Exploring small-scale chemostats to scale up microbial processes: 3-hydroxypropionic acid production in S. cerevisiae ', Microbial Cell Factories, vol. 18, no. 1, 50 . https://doi.org/10.1186/s12934-019-1101-5
BackgroundThe physiological characterization of microorganisms provides valuable information for bioprocess development. Chemostat cultivations are a powerful tool for this purpose, as they allow defined changes to one single parameter at a time, whi
Autor:
Michael Krogh Jensen, Jay D. Keasling, Konstantin Schneider, Arun S. Rajkumar, Jiufu Qin, Emre Özdemir, Alicia V. Lis
Publikováno v:
Rajkumar, A S, Özdemir, E, Lis, A V, Schneider, K, Qin, J, Jensen, M K & Keasling, J D 2019, ' Engineered Reversal of Function in Glycolytic Yeast Promoters ', ACS Synthetic Biology, vol. 8, no. 6, pp. 1462-1468 . https://doi.org/10.1021/acssynbio.9b00027
Promoters are key components of cell factory design, allowing precise expression of genes in a heterologous pathway. Several commonly used promoters in yeast cell factories belong to glycolytic genes, highly expressed in actively growing yeast when g
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::f90143e9aaf5aa1e567c629b66d6d5a0
https://orbit.dtu.dk/en/publications/b64425eb-99ac-45c3-b0ce-3ca871a4c4b9
https://orbit.dtu.dk/en/publications/b64425eb-99ac-45c3-b0ce-3ca871a4c4b9
Autor:
Mariana Beatriz Badia, Alicia V. Lis, Mariel Claudia Gerrard Wheeler, Marcos A. Tronconi, Antonius J. A. van Maris, Cintia Lucía Arias, Robert Mans, Carlos S. Andreo, María F. Drincovich, Veronica G. Maurino
Publikováno v:
The FEBS Journal, 284(4)
CONICET Digital (CONICET)
Consejo Nacional de Investigaciones Científicas y Técnicas
instacron:CONICET
CONICET Digital (CONICET)
Consejo Nacional de Investigaciones Científicas y Técnicas
instacron:CONICET
NAD(P)-malic enzyme (NAD(P)-ME) catalyzes the reversible oxidative decarboxylation of malate to pyruvate, CO2, and NAD(P)H and is present as a multigene family in Arabidopsis thaliana. The carboxylation reaction catalyzed by purified recombinant Arab