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pro vyhledávání: '"Alice Zijderveld"'
Autor:
Niels de Wind, Arno L. J. Gielkens, Anton Berns, Alice Zijderveld, Ben Peeters, Tjeerd S. Kimman
Publikováno v:
Virology. 200:784-790
We mutagenized and characterized a 41-kilobase-pair subgenomic cloned fragment from the unique long (UL) region of pseudorabies virus (PRV). Forty mutant clones, each carrying a single inserted oligonucleotide, were used for cotransfection with overl
Publikováno v:
Journal of Virology. 64:4691-4696
We describe a technique for the systematic inactivation of nonessential genes within the genome of a herpesvirus without the requirement for phenotypic selection. This technique is based on the insertion of an oligonucleotide containing translational