Kinetic study of membrane protein interactions: from three to two dimensions

Autor: Vladimir Adrien, Myriam Reffay, Nicolas Taulier, Alice Verchère, Laura Monlezun, Martin Picard, Arnaud Ducruix, Isabelle Broutin, Frédéric Pincet, Wladimir Urbach

Publikováno v: Scientific Reports, Vol 14, Iss 1, Pp 1-10 (2024)

Abstract Molecular interactions are contingent upon the system’s dimensionality. Notably, comprehending the impact of dimensionality on protein–protein interactions holds paramount importance in foreseeing protein behaviour across diverse scenari

Externí odkaz: https://doaj.org/article/dcc1cdc4396d456b8e500a4c65e83c99

Complexity of the eukaryotic dolichol-linked oligosaccharide scramblase suggested by activity correlation profiling mass spectrometry

Autor: Alice Verchère, Andrew Cowton, Aurelio Jenni, Monika Rauch, Robert Häner, Johannes Graumann, Peter Bütikofer, Anant K. Menon

Publikováno v: Scientific Reports, Vol 11, Iss 1, Pp 1-14 (2021)

Abstract The oligosaccharide required for asparagine (N)-linked glycosylation of proteins in the endoplasmic reticulum (ER) is donated by the glycolipid Glc3Man9GlcNAc2-PP-dolichol. Remarkably, whereas glycosylation occurs in the ER lumen, the initia

Externí odkaz: https://doaj.org/article/7981e168e2f7493c8bf0c63b12ab6050

Tripartite assembly of RND multidrug efflux pumps

Autor: Laetitia Daury, François Orange, Jean-Christophe Taveau, Alice Verchère, Laura Monlezun, Céline Gounou, Ravi K. R. Marreddy, Martin Picard, Isabelle Broutin, Klaas M. Pos, Olivier Lambert

Publikováno v: Nature Communications, Vol 7, Iss 1, Pp 1-8 (2016)

Tripartite efflux systems consist of inner membrane, outer membrane and periplasmic components. Here, Daury et al. reconstitute native versions of RND transporters in nanodiscs and present projection structures emphasizing the role of the periplasmic

Externí odkaz: https://doaj.org/article/eba793de8123457e92c40e3142a2ad4f

Complexity of the eukaryotic dolichol-linked oligosaccharide scramblase suggested by activity correlation profiling mass spectrometry

Autor: Peter Bütikofer, Andrew Cowton, Aurelio Jenni, Anant K. Menon, Monika Rauch, Johannes Graumann, Alice Verchère, Robert Häner

Publikováno v: Scientific Reports
Scientific Reports, Vol 11, Iss 1, Pp 1-14 (2021)
Verchère, Alice; Cowton, Andrew; Jenni, Aurelio; Rauch, Monika; Häner, Robert; Graumann, Johannes; Bütikofer, Peter; Menon, Anant K. (2021). Complexity of the eukaryotic dolichol-linked oligosaccharide scramblase suggested by activity correlation profiling mass spectrometry. Scientific reports, 11(1), p. 1411. Springer Nature 10.1038/s41598-020-80956-0

The canonical pathway ofN-linked protein glycosylation in yeast and humans involves transfer of the oligosaccharide moiety from the glycolipid Glc3Man9GlcNAc2-PP-dolichol to select asparagine residues in proteins that have been translocated into the

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::8611e4c2117076ccc27836bb3aac0c6e
http://europepmc.org/articles/PMC7809446

Biochemical Reconstitution and Characterization of Multicomponent Drug Efflux Transporters

Autor: Martin, Picard, Elena B, Tikhonova, Isabelle, Broutin, Shuo, Lu, Alice, Verchère, Helen I, Zgurskaya

Publikováno v: Methods in molecular biology (Clifton, N.J.). 1700

Efflux pumps are the major determinants in bacterial multidrug resistance. In Gram-negative bacteria, efflux transporters are organized as macromolecular tripartite machineries that span the two-membrane cell envelope of the bacterium. Biochemical da

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=pmid________::4a30f50f51221f7a92c898ca7e4cfe79
https://pubmed.ncbi.nlm.nih.gov/29177829