Zobrazeno 1 - 10
of 22
pro vyhledávání: '"Alice Pawlowski"'
Autor:
Christin Köbler, Nicolas M. Schmelling, Anika Wiegard, Alice Pawlowski, Gopal K. Pattanayak, Philipp Spät, Nina M. Scheurer, Kim N. Sebastian, Florian P. Stirba, Lutz C. Berwanger, Petra Kolkhof, Boris Maček, Michael J. Rust, Ilka M. Axmann, Annegret Wilde
Publikováno v:
Nature Communications, Vol 15, Iss 1, Pp 1-20 (2024)
Abstract The circadian clock of cyanobacteria, which predicts daily environmental changes, typically includes a standard oscillator consisting of proteins KaiA, KaiB, and KaiC. However, several cyanobacteria have diverse Kai protein homologs of uncle
Externí odkaz:
https://doaj.org/article/b7a57a3391a54e828c4078e28c4643a5
Autor:
Christin Köbler, Nicolas M Schmelling, Alice Pawlowski, Philipp Spät, Nina Scheurer, Lutz LCB Berwanger, Boris Macek, Ilka Axmann, Annegret Wilde
This protocol could be used to analyze Kai-protein complexes in Clear-native PAGE perform Western Blot transfers of large native protein complexes with a semi dry blotting system perform the immunodetection of his-tagged proteins with a single monocl
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::3eba8206f4c33196b966dea70cbc79d1
https://doi.org/10.17504/protocols.io.bu67nzhn
https://doi.org/10.17504/protocols.io.bu67nzhn
Autor:
Christin Köbler, Nicolas M Schmelling, Alice Pawlowski, Philipp Spät, Nina Scheurer, Lutz LCB Berwanger, Boris Macek, Ilka Maria IM Axmann, Annegret Wilde
This protocol can be used to - express his-tagged proteins in E. coli cells by using a T7-polymerase based expression system - Lyse cells with sonification - purify target protein by Ni-affinity chromatography with nickel magnetic beads
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::16e803fe8cbed645ac13af8c02708875
https://doi.org/10.17504/protocols.io.bu5bny2n
https://doi.org/10.17504/protocols.io.bu5bny2n
Autor:
Alice Pawlowski
RNA is denatured in 8 M Urea and separated in a polyacrylamide gel electrophoresis (PAGE) based on its molecular weight. Using this method, fragments between 2 to 500 nucleotids can be separated. The migration of the RNA depends on the concentration
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::b875817220c02b5aff81b161a0d3aecc
https://doi.org/10.17504/protocols.io.beemjbc6
https://doi.org/10.17504/protocols.io.beemjbc6
Autor:
Alice Pawlowski
This protocol can be used to hybridize small RNAs (50 - 200 nt) - either from total RNA isolates or from in vitro transcripts - with digoxigenein-labeled probes, followed by detection with chemiluminescence using CDP-star. The protocol follows mainly
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::89beb6d199fc1876fb534a999ea70ce5
https://doi.org/10.17504/protocols.io.us6ewhe
https://doi.org/10.17504/protocols.io.us6ewhe
Autor:
Anni M. Moilanen, Alice Pawlowski, Vesa P. Hytönen, Juha A. E. Määttä, Ilona Rissanen, Jaana K. H. Bamford, Janne A. Ihalainen
Publikováno v:
Journal of Virology. 89:7593-7603
Thermus thermophilus bacteriophage P23-77 is the type member of a new virus family of icosahedral, tailless, inner-membrane-containing double-stranded DNA (dsDNA) viruses infecting thermophilic bacteria and halophilic archaea. The viruses have a uniq
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::82f0e00b779002455fe9a1daa0c8ab2b
https://doi.org/10.1128/jvi.00262-15
https://doi.org/10.1128/jvi.00262-15
Autor:
Alice Pawlowski, Lutz LCB Berwanger
RNA extraction from E. coli cells based on the method described by Chomczynski and Sacchi, 1987
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::d2c645d68745392a666e303c59be1102
https://doi.org/10.17504/protocols.io.kzycx7w
https://doi.org/10.17504/protocols.io.kzycx7w
Autor:
Alice Pawlowski
The protocol is used for the extraction of total RNA from E. coli cells. It is based on the method described by Chomczynski and Sacchi, 1987 (https://doi.org/10.1016/0003-2697(87)90021-2). Total RNA is isolated from cells expressing small regulatory
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::ff2136f347ed357946e75f73b50f24a5
https://doi.org/10.17504/protocols.io.gtnbwme
https://doi.org/10.17504/protocols.io.gtnbwme
Autor:
Anna Behle, Alice Pawlowski
Stock solution for 50x TAE. TAE buffer is a solution made up of Tris base, acetic acid and EDTA (Tris-acetate-EDTA). It is a common bufferfor DNA separation using standard agarose gel electrophoresis.
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::3b7ff1b750ed9417d9f7c67afd2fda0a
https://doi.org/10.17504/protocols.io.gtvbwn6
https://doi.org/10.17504/protocols.io.gtvbwn6