Zobrazeno 1 - 10
of 23
pro vyhledávání: '"Alice Ylikoski"'
Publikováno v:
BioTechniques, Vol 30, Iss 4, Pp 832-845 (2001)
Quantitative RT-PCR (QRT-PCR) enables the sensitive and specific detection of mRNA with a small copy number. We used the QRT-PCR method and dual-label analysis of amplification products for the detection of prostate-specific antigen (PSA) mRNA. The Q
Externí odkaz:
https://doaj.org/article/6c5b8a9910ec4d03987a9207bcc5ed2e
Autor:
Timo Lövgren, Kim Pettersson, Alice Ylikoski, Martti Nurmi, Matti Karp, Kerttu Irjala, Hans Lilja, Jussi Nurmi
Publikováno v:
Europe PubMed Central
Background: Detection or quantification of circulating cancer cells has been proposed as an aid in detection and monitoring of several solid tumors. We investigated the classification accuracy of prostate-specific antigen (PSA) and human glandular ka
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::bcf8ffc1aed2b53084d096a502f98fb4
https://ora.ox.ac.uk/objects/uuid:2d5be1ac-ba5b-4aac-bda2-26f0b7799483
https://ora.ox.ac.uk/objects/uuid:2d5be1ac-ba5b-4aac-bda2-26f0b7799483
Autor:
Hanna-Mari Raussi, Ville Santala, Katariina E.S. Tolvanen, Matti Karp, Perttu Koskinen, Ilkka Hemmilä, Alice Ylikoski
Publikováno v:
International Journal of Hydrogen Energy. 33:5416-5421
The purpose of the research was to investigate the hydrogenase (hydA) gene and the corresponding transcript levels of Clostridium butyricum during continuous hydrogen fermentation. A quantitative real-time PCR (qrt-PCR) method was developed to specif
Publikováno v:
Bioconjugate Chemistry. 19:1269-1273
Four acyclic nucleoside triphosphates (derivatives of cytosine, thymine, 7-deazaadenine, and 7-deazaguanine) labeled with nonluminescent europium, terbium, dysprosium, and samarium chelates of 2,2',2'',2'''-[[4-(4-isothiocyanatophenyl)ethyl]pyridine-
Autor:
Ilkka Hemmilä, Pia Ollikka, Alice Ylikoski, Perttu Koskinen, Jaakko A. Puhakka, Katariina E.S. Tolvanen, Matti Karp
Publikováno v:
International Journal of Hydrogen Energy. 33:542-549
A quantitative real-time PCR (qrt-PCR) method was developed for quantification of a hydrogen-producing Clostridium butyricum strain. The strain was the main hydrogen producer in a mixed culture bioreactor that was operated on a continuous-flow mode f
Autor:
Jari Peuralahti, Harri Hakala, and Alice Ylikoski, Pekka Tallqvist, Veli-Matti Mukkala, Lassi Jaakkola, Jenni Kunttu, Jari Hovinen
Publikováno v:
Bioconjugate Chemistry. 16:700-709
The synthesis of phosphoramidite building blocks that allow introduction of luminescent europium(III), terbium(III), dysprosium(III), and samarium(III) chelates to oligonucleotides on the solid phase is described. Several labeled oligonucleotides usi
Autor:
Jari Hovinen, Pia Ollikka, Veli-Matti Mukkala, Annika Elomaa, Harri Hakala, Alice Ylikoski, Ilkka Hemmilä
Publikováno v:
Clinical Chemistry. 50:1943-1947
Laboratories performing genetic screening studies need simplified methods that allow automation for nucleic acid analysis. Ideally, a method should have as few handling steps as possible and allow the use of a closed-tube assay format to reduce the r
Autor:
Nikolaos Zamboglou, G. Aumüller, Sandra Röddiger, U.W. Tunn, Heiner Renneberg, Alice Ylikoski, Lutz Konrad, Hans Lilja, Ralf Kurek
Publikováno v:
The Prostate. 56:263-269
BACKGROUND. The clinical value of detecting prostate specific antigen (PSA) mRNA in the peripheral blood mononuclear cell fraction of patients (pts) by standard RT-PCR assays with localized prostate cancer remains controversial. We used a quantitativ
Autor:
Harri Hakala, Pia Ollikka, Päivi Liitti, Jarkko Karvinen, Katri Puukka, Jari Peuralahti, Veli-Matti Mukkala, Alice Ylikoski, Kristiina Loman, Jari Hovinen
Publikováno v:
Inorganic Chemistry Communications. 5:1059-1062
A series of new stable, luminescent samarium(III) chelates were synthesized and their photophysical properties were measured. The iodoacetamido activated chelate coupled to a peptide was used in a caspase-3 assay.
Publikováno v:
The Journal of Molecular Diagnostics. 3:111-122
We present a multiplexed and internally calibrated quantitative reverse transcription-PCR (QRT-PCR) assay to detect human glandular kallikrein 2 (hK2) and prostate-specific antigen (PSA) transcripts in blood samples from healthy subjects and prostate