Zobrazeno 1 - 10
of 20
pro vyhledávání: '"Ali Punjani"'
Autor:
Ali Punjani, David J. Fleet
Publikováno v:
Nature Methods.
Modeling flexible macromolecules is one of the foremost challenges in single-particle cryogenic-electron microscopy (cryo-EM), with the potential to illuminate fundamental questions in structural biology. We introduce Three-Dimensional Flexible Refin
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::32d27fd3cb255340a207d0f879fae5d6
https://doi.org/10.1038/s41592-023-01853-8
https://doi.org/10.1038/s41592-023-01853-8
Autor:
Tristan Bepler, Andrew J. Borst, Jonathan Bouvette, Giuseppe Cannone, Songye Chen, Anchi Cheng, Ao Cheng, Quanfu Fan, Fanis Grollios, Harshit Gupta, Meghna Gupta, Theo Humphreys, Paul T. Kim, Huihui Kuang, Yilai Li, Alex J. Noble, Ali Punjani, William J. Rice, Carlos Oscar S. Sorzano, Scott M. Stagg, Joshua Strauss, Lingbo Yu, Bridget Carragher, Clinton S. Potter
Publikováno v:
Journal of structural biology. 214(4)
This report provides an overview of the discussions, presentations, and consensus thinking from the Workshop on Smart Data Collection for CryoEM held at the New York Structural Biology Center on April 6-7, 2022. The goal of the workshop was to addres
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::65e2fcb84003f84ad7b748d599572c7a
https://pubmed.ncbi.nlm.nih.gov/36341954
https://pubmed.ncbi.nlm.nih.gov/36341954
Publikováno v:
Nature Methods. 17:1214-1221
Cryogenic electron microscopy (cryo-EM) is widely used to study biological macromolecules that comprise regions with disorder, flexibility or partial occupancy. For example, membrane proteins are often kept in solution with detergent micelles and lip
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::8989f04b9a65160005d0b7708e38b237
https://doi.org/10.1038/s41592-020-00990-8
https://doi.org/10.1038/s41592-020-00990-8
Autor:
Ali Punjani, David Fleet
Publikováno v:
Microscopy and Microanalysis. 28:1218-1218
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::c5a8fd9a95dd66826483a5c634723dc9
https://doi.org/10.1017/s1431927622005074
https://doi.org/10.1017/s1431927622005074
Autor:
David J. Fleet, Ali Punjani
Single particle cryo-EM excels in determining static structures of biological macromolecules such as proteins. However, many proteins are dynamic, with their motion inherently linked to their function. Recovering the continuous motion and detailed 3D
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::22ae94e1b00c4b46244cfeef6f6b1485
https://doi.org/10.1101/2021.04.22.440893
https://doi.org/10.1101/2021.04.22.440893
Autor:
Ali Punjani
Publikováno v:
Microscopy and Microanalysis. 27:2592-2593
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::c131220d5984ba8d3483a6f94bdec8e2
https://doi.org/10.1017/s1431927621009211
https://doi.org/10.1017/s1431927621009211
Autor:
David J. Fleet, Ali Punjani
Publikováno v:
Acta Crystallographica Section A Foundations and Advances. 77:a235-a235
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::3cdea7d1e46d3cca4b76785b89620ceb
https://doi.org/10.1107/s0108767321097646
https://doi.org/10.1107/s0108767321097646
Autor:
David J. Fleet, Ali Punjani
Publikováno v:
Journal of Structural Biology. 213:107702
Single particle cryo-EM excels in determining static structures of protein molecules, but existing 3D reconstruction methods have been ineffective in modelling flexible proteins. We introduce 3D variability analysis (3DVA), an algorithm that fits a l
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::dac0f0e594f1876cde1572d4d8b08adf
https://doi.org/10.1016/j.jsb.2021.107702
https://doi.org/10.1016/j.jsb.2021.107702
Building Proteins in a Day: Efficient 3D Molecular Structure Estimation with Electron Cryomicroscopy
Publikováno v:
IEEE Transactions on Pattern Analysis and Machine Intelligence. 39:706-718
Discovering the 3D atomic-resolution structure of molecules such as proteins and viruses is one of the foremost research problems in biology and medicine. Electron Cryomicroscopy (cryo-EM) is a promising vision-based technique for structure estimatio
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::6572f66fd7817f190ed84f441cdbda61
https://doi.org/10.1109/tpami.2016.2627573
https://doi.org/10.1109/tpami.2016.2627573
Publikováno v:
Nature Methods. 14:290-296
Single-particle electron cryomicroscopy (cryo-EM) is a powerful method for determining the structures of biological macromolecules. With automated microscopes, cryo-EM data can often be obtained in a few days. However, processing cryo-EM image data t
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::3ccfa851494220dbad586647a1ee4571
https://doi.org/10.1038/nmeth.4169
https://doi.org/10.1038/nmeth.4169