Role of error-prone DNA polymerases in spontaneous mutagenesis in Caulobacter crescentus

Autor: Vânia Santos Braz, Rodrigo S. Galhardo, Alexy O. Valencia, Magna Magalhães

Publikováno v: Genetics and Molecular Biology, Volume: 43, Issue: 1, Article number: e20180283, Published: 09 MAR 2020
Genetics and Molecular Biology
Genetics and Molecular Biology v.43 n.1 2020
Sociedade Brasileira de Genética (SBG)
instacron:SBG
Repositório Institucional da USP (Biblioteca Digital da Produção Intelectual)
Universidade de São Paulo (USP)
instacron:USP

Spontaneous mutations are important players in evolution. Nevertheless, there is a paucity of information about the mutagenic processes operating in most bacterial species. In this work, we implemented two forward mutational markers for studies in Ca

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::5a56fabdfb28aefcee8f42736d83f753
http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572020000100402&lng=en&tlng=en

Molecular characterization of Caulobacter crescentus mutator strains

Autor: Marinalva Martins-Pinheiro, Frank S. Fernandez-Silva, Alice R. Oliveira, Valéria C. S. Italiani, Rodrigo S. Galhardo, Carlos Frederico Martins Menck, Carina O. Lopes-Kulishev, Alexy O. Valencia, Larissa G. Silva, Marilis V. Marques

Publikováno v: Repositório Institucional da USP (Biblioteca Digital da Produção Intelectual)
Universidade de São Paulo (USP)
instacron:USP

Mutator strains were identified by screening random Tn5 insertion clones of Caulobacter crescentus. We identified clones with robust increases in mutation rates with Tn5 insertions in the mutY, mutS, mutL and uvrD genes, known to act in mutation-prev

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::d8a6270de3b1ab0e2079c4de0e2d4114