Zobrazeno 1 - 10
of 40
pro vyhledávání: '"Alexey Y. Karulin"'
Autor:
Paul V. Lehmann, Maneewan Suwansaard, Ting Zhang, Diana R. Roen, Greg A. Kirchenbaum, Alexey Y. Karulin, Alexander Lehmann, Pedro A. Reche
Publikováno v:
Frontiers in Immunology, Vol 10 (2019)
T cell immunity is traditionally assessed through functional recall assays, which detect the consequences of the T cells' antigen encounter, or via fluorescently labeled multimers that selectively bind peptide-specific T cell receptors. Using either
Externí odkaz:
https://doaj.org/article/082307207f73445d8689f2a80034651d
Autor:
Marie Wunsch, Wenji Zhang, Jodi Hanson, Richard Caspell, Alexey Y. Karulin, Mascha S. Recks, Stefanie Kuerten, Srividya Sundararaman, Paul V. Lehmann
Publikováno v:
Viruses, Vol 7, Iss 8, Pp 4414-4437 (2015)
Most humans become infected with human cytomegalovirus (HCMV). Typically, the immune system controls the infection, but the virus persists and can reactivate in states of immunodeficiency. While substantial information is available on the contributio
Externí odkaz:
https://doaj.org/article/51e0bee4a5e54b91a2acc027775c046b
Publikováno v:
Cells, Vol 4, Iss 1, Pp 96-111 (2015)
Accurate assessment of positive ELISPOT responses for low frequencies of antigen-specific T-cells is controversial. In particular, it is still unknown whether ELISPOT counts within replicate wells follow a theoretical distribution function, and thus
Externí odkaz:
https://doaj.org/article/7aff19f029fb40d782bc59b8d4954b32
Autor:
Alexey Y. Karulin, Kinga Karacsony, Wenji Zhang, Oleg S. Targoni, Ioana Moldovan, Marcus Dittrich, Srividya Sundararaman, Paul V. Lehmann
Publikováno v:
Cells, Vol 4, Iss 1, Pp 56-70 (2015)
Each positive well in ELISPOT assays contains spots of variable sizes that can range from tens of micrometers up to a millimeter in diameter. Therefore, when it comes to counting these spots the decision on setting the lower and the upper spot size t
Externí odkaz:
https://doaj.org/article/33095d56ff8e4a7b9673fa575e56d54a
Autor:
Srividya Sundararaman, Alexey Y. Karulin, Tameem Ansari, Nadine BenHamouda, Judith Gottwein, Sreenivas Laxmanan, Steven M. Levine, John T. Loffredo, Stephanie McArdle, Christine Neudoerfl, Diana Roen, Karina Silina, Mackenzie Welch, Paul V. Lehmann
Publikováno v:
Cells, Vol 4, Iss 1, Pp 21-39 (2015)
The primary goal of immune monitoring with ELISPOT is to measure the number of T cells, specific for any antigen, accurately and reproducibly between different laboratories. In ELISPOT assays, antigen-specific T cells secrete cytokines, forming spots
Externí odkaz:
https://doaj.org/article/4b8b577842b84291b39e2adf1401c336
Autor:
Jodi Hanson, Srividya Sundararaman, Richard Caspell, Edith Karacsony, Alexey Y. Karulin, Paul V. Lehmann
Publikováno v:
Cells, Vol 4, Iss 1, Pp 71-83 (2015)
Comprehensive immune monitoring requires that frequencies of T cells, producing different cytokines, are measured to establish the magnitude of Th1, Th2, and Th17 components of cell-mediated immunity. Antigen titration provides additional information
Externí odkaz:
https://doaj.org/article/aa248226d1c64f3fbc2bbae4cfc64818
Publikováno v:
Cells, Vol 1, Iss 3, Pp 576-596 (2012)
Experimental allergic encephalomyelitis (EAE) is mediated by neuroantigen-specific pro-inflammatory T cells of the Th1 and Th17 effector class. Th-17 cells can be clearly defined by expression of IL-17, but not IFN-γ, IL-2 or IL-3. Th1 cells do not
Externí odkaz:
https://doaj.org/article/f47d980a4b8d4b35af574f80411b24c2
Autor:
Philipp Fecher, Richard Caspell, Villian Naeem, Alexey Y. Karulin, Stefanie Kuerten, Paul V. Lehmann
Publikováno v:
Cells, Vol 7, Iss 6, p 50 (2018)
In individuals who have once developed humoral immunity to an infectious/foreign antigen, the antibodies present in their body can mediate instant protection when the antigen re-enters. Such antigen-specific antibodies can be readily detected in the
Externí odkaz:
https://doaj.org/article/5e6b46e4d6624a7abde1e80b3962e753
High-Throughput GLP-Capable Target Cell Visualization Assay for Measuring Cell-Mediated Cytotoxicity
Autor:
Anna Welter, Srividya Sundararaman, Ruliang Li, Ting Zhang, Alexey Y. Karulin, Alexander Lehmann, Villian Naeem, Diana R. Roen, Stefanie Kuerten, Paul V. Lehmann
Publikováno v:
Cells, Vol 7, Iss 5, p 35 (2018)
One of the primary effector functions of immune cells is the killing of virus-infected or malignant cells in the body. Natural killer (NK) and CD8 effector T cells are specialized for this function. The gold standard for measuring such cell-mediated
Externí odkaz:
https://doaj.org/article/d095e72374004c84b83fe14e68e4cd34
Autor:
Alexander A. Lehmann, Greg A. Kirchenbaum, Alexey Y. Karulin, Diana R. Roen, Maneewan Suwansaard, Ting Zhang, Paul V. Lehmann, Pedro A. Reche
Publikováno v:
Frontiers in Immunology
Frontiers in Immunology, Vol 10 (2019)
Frontiers in Immunology, Vol 10 (2019)
T cell immunity is traditionally assessed through functional recall assays, which detect the consequences of the T cells' antigen encounter, or via fluorescently labeled multimers that selectively bind peptide-specific T cell receptors. Using either
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::d41459c6ea6c85671b793dc5a3459d8b
https://pubmed.ncbi.nlm.nih.gov/31105686
https://pubmed.ncbi.nlm.nih.gov/31105686