RNAi-mediated depletion of the NSL complex subunits leads to abnormal chromosome segregation and defective centrosome duplication in Drosophila mitosis.

Autor: Gera A Pavlova, Julia V Popova, Evgeniya N Andreyeva, Lyubov A Yarinich, Mikhail O Lebedev, Alyona V Razuvaeva, Tatiana D Dubatolova, Anastasiya L Oshchepkova, Claudia Pellacani, Maria Patrizia Somma, Alexey V Pindyurin, Maurizio Gatti

Publikováno v: PLoS Genetics, Vol 15, Iss 9, p e1008371 (2019)

The Drosophila Nonspecific Lethal (NSL) complex is a major transcriptional regulator of housekeeping genes. It contains at least seven subunits that are conserved in the human KANSL complex: Nsl1/Wah (KANSL1), Dgt1/Nsl2 (KANSL2), Rcd1/Nsl3 (KANSL3),

Externí odkaz: https://doaj.org/article/74f0d8efc5954cc9885613dccef45182

Optimized PCR conditions minimizing the formation of chimeric DNA molecules from MPRA plasmid libraries

Autor: Evgeniya S. Omelina, Anton V. Ivankin, Anna E. Letiagina, Alexey V. Pindyurin

Publikováno v: BMC Genomics, Vol 20, Iss S7, Pp 1-10 (2019)

Abstract Background Massively parallel reporter assays (MPRAs) enable high-throughput functional evaluation of various DNA regulatory elements and their mutant variants. The assays are based on construction of highly diverse plasmid libraries contain

Externí odkaz: https://doaj.org/article/ae2faaa1610d41db9ffeb8a9878a9bf9

The role of Patronin in Drosophila mitosis

Autor: Gera A. Pavlova, Alyona V. Razuvaeva, Julia V. Popova, Evgeniya N. Andreyeva, Lyubov A. Yarinich, Mikhail O. Lebedev, Claudia Pellacani, Silvia Bonaccorsi, Maria Patrizia Somma, Maurizio Gatti, Alexey V. Pindyurin

Publikováno v: BMC Molecular and Cell Biology, Vol 20, Iss S1, Pp 1-13 (2019)

Abstract Background The calmodulin-regulated spectrin-associated proteins (CAMSAPs) belong to a conserved protein family, which includes members that bind the polymerizing mcrotubule (MT) minus ends and remain associated with the MT lattice formed by

Externí odkaz: https://doaj.org/article/82654c3f397a41b3a69ffb2e63979cb8

Optogenetic and Chemical Induction Systems for Regulation of Transgene Expression in Plants: Use in Basic and Applied Research

Autor: Evgeniya S. Omelina, Anastasiya A. Yushkova, Daria M. Motorina, Grigorii A. Volegov, Elena N. Kozhevnikova, Alexey V. Pindyurin

Publikováno v: International Journal of Molecular Sciences, Vol 23, Iss 3, p 1737 (2022)

Continuous and ubiquitous expression of foreign genes sometimes results in harmful effects on the growth, development and metabolic activities of plants. Tissue-specific promoters help to overcome this disadvantage, but do not allow one to precisely

Externí odkaz: https://doaj.org/article/db3d529fd88c41c3851daa25e8ab3e81

The multiple mitotic roles of the ASPM orthologous proteins:Insight into the etiology of ASPM-dependent microcephaly

Autor: Alyona V. Razuvaeva, Lucia Graziadio, Valeria Palumbo, Gera A. Pavlova, Julia V. Popova, Alexey V. Pindyurin, Silvia Bonaccorsi, Maria Patrizia Somma, Maurizio Gatti

Publikováno v: Razuvaeva, A V, Graziadio, L, Palumbo, V, Pavlova, G A, Popova, J V, Pindyurin, A V, Bonaccorsi, S, Somma, M P & Gatti, M 2023, ' The multiple mitotic roles of the ASPM orthologous proteins : Insight into the etiology of ASPM-dependent microcephaly ', Cells, vol. 12, no. 6, 922 . https://doi.org/10.3390/cells12060922

The Drosophila abnormal spindle (asp) gene was discovered about 40 years ago and shown to be required for both mitotic and meiotic cell division. Subsequent studies showed that asp is highly conserved and that mutations in its human ortholog ASPM (Ab

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::341ae0b12190e09652f33770f1e9e8b8
https://hdl.handle.net/20.500.11820/259c7c88-7964-4753-902d-3985ee2317f9