Zobrazeno 1 - 10
of 13
pro vyhledávání: '"Alexey Chernogolov"'
Autor:
Spencer S. Ericksen, Horst Honeck, Dieter Schwarz, Pyotr Kisselev, Wolf-Hagen Schunck, Grazyna D. Szklarz, Alexey Chernogolov, Ivar Roots
Publikováno v:
Biochemical Pharmacology. 67:1445-1457
Human cytochrome P450 1A1 (CYP1A1) and human NADPH-cytochrome P450 reductase were expressed and purified from Spodoptera frugiperda insect cells. A reconstituted enzymatically active system metabolized polyunsaturated fatty acids such as arachidonic
Publikováno v:
Protein Expression and Purification. 28:259-269
Three human cytochrome P450 1A1 (CYP1A1) allelic variants, namely wild-type (CYP1A1.1), CYP1A1.2 (I462V), and CYP1A1.4 (T461N), were expressed as C-terminal His-tagged fusions including a thrombin cleavage site in Spodoptera frugiperda insect cells b
Publikováno v:
Journal of Pharmaceutical and Biomedical Analysis. 15:1231-1240
With fluorescence microscopic imaging, we have examined Ca 2+ signaling, LDL uptake and distribution of cytochrome P 450 scc in individual rat brain glial cells in order to investigate the molecular mechanisms of neurosteroid synthesis. Astrocytes an
Autor:
Svetlana Dubrovskaya, T. Dubrovsky, Claudio Nicolini, Alexey Chernogolov, Sergei A. Usanov, Oleg Guryev
Publikováno v:
Langmuir. 13:299-304
Monolayers of cytochrome P450scc and its complex with adrenodoxin were formed by Langmuir techniques and covalently immobilized on the solid substrates. The orientation of hemeprotein molecules was studied using polyclonal antibodies specific to the
Publikováno v:
Journal of Structural Biology. 113:207-215
Cytochrome P450scc can be reconstituted successfully into large unilamellar phospholipid vesicles by a combined octylglucoside dialysis/adsorption method. Freeze-fracture electron microscopy was used to analyze the morphology, distribution, and prote
Publikováno v:
Biochemical and Biophysical Research Communications. 195:889-896
The rotational diffusion of cytochrome P450SCC (CYP45011A1) was investigated in proteoliposomes measuring the time-resolved delayed fluorescence anisotropy of diiodofluorescein iodoacetamide covalently and specifically attached to Cys264 of P450SCC.
Publikováno v:
Biochemical and biophysical research communications. 336(3)
To answer the question whether the most common allelic variants of human CYP1A1, namely CYP1A1.1 (wild type), CYP1A1.2 (Ile462Val), and CYP1A1.4 (Thr461Asn), differ in their catalytic activity towards eicosapentaenoic acid (EPA), in vitro enzymatic a
Autor:
Werner Boidol, Pyotr Kisselev, Alexey Chernogolov, Dieter Schwarz, Ivar Roots, Ingolf Cascorbi, Wolf-Hagen Schunck
Publikováno v:
Pharmacogenetics. 10(6)
Steroid hydroxylation specificities were determined for the wild-type and the two allelic variants of the polymorphic human cytochrome P450 1A1 (CYP1A1) that were associated with amino acid exchanges near the active site of the enzyme. All three vari
Publikováno v:
Biochemistry. 38(29)
Rotational diffusion measurements using EPR and saturation transfer EPR were applied to analyze complex formation between the electron-transfer components of the mitochondrial steroid-hydroxylating cytochrome P450 systems (CYP11A1 and CYP11B1) in pho
Publikováno v:
Archives of biochemistry and biophysics. 359(2)
Eukaryotic P450 proteins are membrane proteins found predominantly in the endoplasmic reticulum. In vertebrates, several biosynthetic P450s are found in mitochondria as well. We cloned three putative insect mitochondrial P450s from larval house fly c