Výsledky vyhledávání - "Alexey Atrazhev"

DNA-Encoded Multivalent Display of Chemically Modified Protein Tetramers on Phage: Synthesis and

Autor: Guilherme M, Lima, Alexey, Atrazhev, Susmita, Sarkar, Mirat, Sojitra, Revathi, Reddy, Karin, Torres-Obreque, Carlota, de Oliveira Rangel-Yagui, Matthew S, Macauley, Gisele, Monteiro, Ratmir, Derda

Publikováno v: ACS chemical biology. 17(11)

Phage display links the phenotype of displayed polypeptides with the DNA sequence in the phage genome and offers a universal method for the discovery of proteins with novel properties. However, the display of large multisubunit proteins on phages rem

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=pmid________::99e8193bcf8448be989c29dc679b2fe4
https://pubmed.ncbi.nlm.nih.gov/34928124

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DNA-encoded multivalent display of protein tetramers on phage: synthesis and in vivo applications

Autor: Guilherme M. Lima, Susmita Sarkar, Mirat Sojitra, Gisele Monteiro, Alexey Atrazhev, Revathi Reddy, Matthew S. Macauley, Ratmir Derda

Publikováno v: Repositório Institucional da USP (Biblioteca Digital da Produção Intelectual)
Universidade de São Paulo (USP)
instacron:USP

Phage display links phenotype of displayed polypeptides with DNA sequence in phage genome and offers a universal method for discovery of proteins with novel properties. Injection of phage-displayed libraries in living organisms further provides a uni

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::ee171cb2a1767227674b97525ca7a42b

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Inhibition of on-chip PCR using PDMS–glass hybrid microfluidic chips

Autor: R.W. Johnstone, Linda M. Pilarski, H. John Crabtree, Yuen Morrissey, Alexey Atrazhev, Christopher J. Backhouse, Jana Lauzon, Alexander J. Stickel, Brian J. Taylor, Dammika P. Manage, Tina Liang

Publikováno v: Microfluidics and Nanofluidics. 13:383-398

In the course of developing a microfluidic analytical platform incorporating the polymerase chain reaction (PCR) and subsequent capillary electrophoresis (CE) analysis for a variety of bio-assays, we examined PCR inhibition through surface interactio

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_________::ecbc60b0abba0dab6e18aaeb32636e33
https://doi.org/10.1007/s10404-012-0968-9

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On-chip PCR amplification of genomic and viral templates in unprocessed whole blood

Autor: Alexander J. Stickel, Jason P. Acker, Alexey Atrazhev, Dammika P. Manage, Linda M. Pilarski, Jana Lauzon, Yuen C. Morrissey

Publikováno v: Microfluidics and Nanofluidics. 10:697-702

Performing medical diagnosis in microfluidic devices could scale down laboratory functions and reduce the cost for accessible healthcare. The ultimate goal of such devices is to receive a sample of blood, perform genetic amplification (polymerase cha

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_________::0ecc651fb4b8f54155bbe5492bb49d68
https://doi.org/10.1007/s10404-010-0702-4

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Microfluidic Platform for Single Nucleotide Polymorphism Genotyping of the Thiopurine S-Methyltransferase Gene to Evaluate Risk for Adverse Drug Events

Autor: Jeeshan Chowdhury, Christopher J. Backhouse, Alexey Atrazhev, Jana Lauzon, Linda M. Pilarski, Alistair J Makin, Alex Stickel, Govind V. Kagiala, Sudeep Pushpakom, William G. Newman

Publikováno v: The Journal of Molecular Diagnostics. 9:521-529

Prospective clinical pharmacogenetic testing of the thiopurine S-methyltransferase gene remains to be realized despite the large body of evidence demonstrating clinical benefit for the patient and cost effectiveness for health care systems. We descri

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_________::035c73a933c95d8d08e20e7badc92158
https://doi.org/10.2353/jmoldx.2007.070014

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Anti-BSA Antibodies do not Cross-react with the 69-kDa Islet Cell Autoantigen ICA69

Autor: Alexey Atrazhev, John F. Elliott, Ray V. Rajotte, Colin Luo, Dean K. Smith, Linda Luo, Gregory S. Korbutt, Kjersti S Rønningen

Publikováno v: Journal of Autoimmunity. 11:223-231

In contrast to several previously published reports, we demonstrate by a variety of antibody assays that bovine serum albumin (BSA) is not antigenically cross-reactive with the 69-kDa islet cell autoantigen (ICA69). Fast protein liquid chromatography

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::268aec85c37d69366b94be14c92ff1d5
https://doi.org/10.1006/jaut.1998.0199

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A novel method for sample delivery and testing of whole blood: gel strip PCR for point of care (POC) molecular diagnostics

Autor: Xiaoli Pang, Linda M. Pilarski, Jana Lauzon, Alexey Atrazhev, Dammika P. Manage

Publikováno v: Lab on a chip. 13(20)

Testing of whole blood in miniaturized PCR is compromised by the opaque nature of whole blood that leads to physical masking of a fluorescent signal. We demonstrate a method to perform real-time PCR with whole blood that avoids interference from the

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::d56cabdcf48fdd7d2f1732fe5fe983fc
https://pubmed.ncbi.nlm.nih.gov/23966268

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In-gel technology for PCR genotyping and pathogen detection

Autor: Alexey Atrazhev, Dammika P. Manage, Jason P. Acker, Alexander J. Stickel, H. John Crabtree, Linda M. Pilarski

Publikováno v: Analytical chemistry. 82(19)

This work describes the use of polyacrylamide gel and PCR reagents photopolymerized in a mold to create an array of semisolid posts that serve as reaction vessels for parallel PCR amplification of an externally added template. DNA amplification occur

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::c6d8a474400f77a11664f9f5bd9f77f9
https://pubmed.ncbi.nlm.nih.gov/20809605

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Microfluidic chips for detecting the t(4;14) translocation and monitoring disease during treatment using reverse transcriptase-polymerase chain reaction analysis of IgH-MMSET hybrid transcripts

Autor: Sophia Adamia, Govind V. Kaigala, Alexey Atrazhev, Brian J. Taylor, Jaron VanDijken, Linda M. Pilarski, Christopher J. Backhouse, Tony Reiman, Andrew R. Belch, Jana Lauzon

Publikováno v: The Journal of molecular diagnostics : JMD. 9(3)

Diagnosis platforms incorporating low-cost microfluidic chips enable sensitive, rapid, and accurate genetic analysis that could facilitate customized therapies tailored to match the vulnerabilities of any types of cancer. Using ex vivo cancer cells,

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::9210eec0b843d0d2789d6e34f3986591
https://pubmed.ncbi.nlm.nih.gov/17591936

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