Zobrazeno 1 - 10
of 21
pro vyhledávání: '"Alexei S Kazakov"'
Autor:
Anna V Lomonosova, Andrei B Ulitin, Alexei S Kazakov, Tajib A Mirzabekov, Eugene A Permyakov, Sergei E Permyakov
Publikováno v:
PLoS ONE, Vol 12, Iss 1, p e0170349 (2017)
Small antibody mimetics, or alternative binding proteins (ABPs), extend and complement antibody functionality with numerous applications in research, diagnostics and therapeutics. Given the superiority of ABPs, the last two decades have witnessed dev
Externí odkaz:
https://doaj.org/article/b2cb9c993d7741e0838077d0271c1463
Autor:
Anna V Lomonosova, Elena V Ovchinnikova, Alexei S Kazakov, Alexander I Denesyuk, Alexander D Sofin, Roman V Mikhailov, Andrei B Ulitin, Tajib A Mirzabekov, Eugene A Permyakov, Sergei E Permyakov
Publikováno v:
PLoS ONE, Vol 10, Iss 8, p e0134906 (2015)
Due to their remarkably high structural stability, proteins from extremophiles are particularly useful in numerous biological applications. Their utility as alternative protein scaffolds could be especially valuable in small antibody mimetic engineer
Externí odkaz:
https://doaj.org/article/69dc8a9ab4a84808b4790602bfa4e9e2
Autor:
Sergey E. Permyakov, Alisa S. Vologzhannikova, Ekaterina L. Nemashkalova, Alexei S. Kazakov, Alexander I. Denesyuk, Konstantin Denessiouk, Viktoriia E. Baksheeva, Andrey A. Zamyatnin, Evgeni Yu. Zernii, Vladimir N. Uversky, Eugene A. Permyakov
Publikováno v:
Molecules, Vol 24, Iss 13, p 2494 (2019)
Recently, we have found that calcium binding proteins of the EF-hand superfamily (i.e., a large family of proteins containing helix-loop-helix calcium binding motif or EF-hand) contain two types of conserved clusters called cluster I (‘black’ clu
Externí odkaz:
https://doaj.org/article/aebbd46b5c74438f8292075676a5a1c4
Autor:
Andrey S Sokolov, Oxana V. Galzitskaya, Ekaterina L. Nemashkalova, Eugene A. Permyakov, Alexei S. Kazakov, Ekaterina A. Litus, E.I. Galushko, S.E. Permyakov, Victor V. Marchenkov, Ulyana F. Dzhus
Publikováno v:
Biochemical and Biophysical Research Communications. 510:248-253
Human serum albumin (HSA) serves as a natural depot of amyloid β peptide (Aβ). Improvement of Aβ binding to HSA should impede Alzheimer's disease (AD). We developed a method for quantitation of the interaction between monomeric Aβ40/42 and HSA us
Autor:
Alexei S. Kazakov, Polina Khorn, Alisa A. Vologzhannikova, Eugene A. Permyakov, Vladimir N. Uversky, Sergei E. Permyakov
Publikováno v:
Cell Calcium. 77:1-7
A comparative study of His-tagged and non-tagged rat β-parvalbumin (rWT β-PA), calcium binding protein with the EF-hand calcium binding domains, has been carried out. The attachment of His-tag increases α-helical content and decreases β-sheets an
Autor:
Sergei E. Permyakov, Marina P. Shevelyova, Eugene A. Permyakov, Maria E. Permyakova, Ramis G. Ismailov, Ekaterina A. Litus, Alexei S. Kazakov
Publikováno v:
International Journal of Biological Macromolecules. 108:143-148
S100 proteins are multifunctional (intra/extra)cellular mostly dimeric calcium-binding proteins engaged into numerous diseases. We have found that monomeric recombinant human S100P protein interacts with intact human serum albumin (HSA) in excess of
Autor:
Vladimir N. Uversky, Elena N. Yundina, Sergei E. Permyakov, Maria E. Permyakova, Eugene A. Permyakov, Alexei S. Kazakov
Publikováno v:
Cell calcium. 87
Bovine S100 G (calbindin D9k, small Ca2+-binding protein of the EF-hand superfamily) is considered as a calcium buffer protein; i.e., the binding of Ca2+ practically does not change its general conformation. A set of experimental approaches has been
Autor:
Vladimir N. Uversky, Alexei S. Kazakov, Alexander I. Denesyuk, Andrey A. Zamyatnin, S.E. Permyakov, Ekaterina L. Nemashkalova, Viktoriia E. Baksheeva, Alisa S. Vologzhannikova, Konstantin Denessiouk, Eugene A. Permyakov, Evgeni Yu. Zernii
Publikováno v:
Molecules, Vol 24, Iss 13, p 2494 (2019)
Molecules
Volume 24
Issue 13
Molecules
Volume 24
Issue 13
Recently, we have found that calcium binding proteins of the EF-hand superfamily (i.e., a large family of proteins containing helix-loop-helix calcium binding motif or EF-hand) contain two types of conserved clusters called cluster I (&lsquo
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Autor:
Alexander I. Denesyuk, Alexei S. Kazakov, Sergei E. Permyakov, Eugene A. Permyakov, Maria E. Permyakova, Vladimir N. Uversky, Konstantin Denessiouk
Publikováno v:
Cell calcium. 80
Two highly conserved structural motifs observed in members of the EF-hand family of calcium binding proteins. The motifs provide a supporting scaffold for the Ca2+ binding loops and contribute to the hydrophobic core of the EF-hand domain. Each struc
Autor:
Sergei E. Permyakov, Tajib Mirzabekov, Andrei S. Sokolov, Roman Mikhailov, Andrey R. Yakovenko, Alexei S. Kazakov, Ramis G. Ismailov, Eugene A. Permyakov, Ulitin Andrei Borisovich, Solovyev Valery Vladimirovich, Victoria A. Rastrygina
Publikováno v:
Biochemical and Biophysical Research Communications. 468:733-738
Interleukin-11 (IL-11) and S100P are oncoproteins co-expressed in numerous cancers, which might favor their interaction during oncogenesis. We have explored the possibility of this interaction by surface plasmon resonance spectroscopy, intrinsic fluo