Zobrazeno 1 - 10
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pro vyhledávání: '"Alexei A, Yeliseev"'
Autor:
Alexei A, Yeliseev
Publikováno v:
Methods in molecular biology (Clifton, N.J.). 2268
G protein-coupled receptors (GPCR) are integral membrane proteins that regulate multiple cellular processes. To obtain insights into structural properties of GPCR and mechanism of activity, these proteins should be isolated in significant (milligram)
Site-selective labeling and electron paramagnetic resonance studies of human cannabinoid receptor CB
Autor:
Alexei A, Yeliseev, Kaeli, Zoretich, Levi, Hooper, Walter, Teague, Lioudmila, Zoubak, Kirk G, Hines, Klaus, Gawrisch
Publikováno v:
Biochim Biophys Acta Biomembr
Integral membrane G protein-coupled receptors (GPCR) regulate multiple physiological processes by transmitting signals from extracellular milieu to intracellular proteins and are major targets of pharmaceutical drug development. Since GPCR are inhere
METHODS FOR RECOMBINANT EXPRESSION AND FUNCTIONAL CHARACTERIZATION OF HUMAN CANNABINOID RECEPTOR CB2
Autor:
Alexei A. Yeliseev
Publikováno v:
Computational and Structural Biotechnology Journal, Vol 6, Iss 7 (2013)
Cannabinoid receptor CB2 is a seven transmembrane-domain integral membrane protein that belongs to a large superfamily of G protein-coupled receptors (GPCR). CB2 is a part of the endocannabinoid system that plays vital role in regulation of immune re
Externí odkaz:
https://doaj.org/article/4d054e7ffcf5454e87dfa01ebbcf7195
Publikováno v:
J Biol Chem
G protein–coupled receptors (GPCRs) comprise a large class of integral membrane proteins involved in the regulation of a broad spectrum of physiological processes and are a major target for pharmaceutical drug development. Structural studies can he
Publikováno v:
Biochemistry. 45:15583-15590
We report on a novel reconstitution method for G-protein-coupled receptors (GPCRs) that yields detergent-free, single, tubular membranes in porous anodic aluminum oxide (AAO) filters at concentrations sufficient for structural studies by solid-state
Publikováno v:
Methods in molecular biology (Clifton, N.J.). 1177
Tandem affinity purification has been increasingly applied to isolation of recombinant proteins. It relies on two consecutive chromatographic steps that take advantage of the affinity tags placed at opposing ends of the target protein. This allows fo
Autor:
Alexei A. Yeliseev, Samuel Kaplan
Publikováno v:
Journal of Biological Chemistry. 275:5657-5667
The function and specific structural aspects of the tryptophan-rich sensory protein (TspO) of Rhodobacter sphaeroides 2.4.1 were studied using site-directed mutagenesis involving some 17 different amino acids. The choice of these amino acids changes
Autor:
Alexei A. Yeliseev, Samuel Kaplan
Publikováno v:
Journal of Biological Chemistry. 274:21234-21243
A bacterial homolog of the mammalian mitochondrial benzodiazepine receptor, the tryptophan-rich sensory protein (TspO) has been previously demonstrated to negatively affect the transcriptional expression of several photosynthesis genes of Rhodobacter
Publikováno v:
Proceedings of the National Academy of Sciences. 94:5101-5106
The rat mitochondrial outer membrane-localized benzodiazepine receptor (MBR) was expressed in wild-type and TspO − (tryptophan-rich sensory protein) strains of the facultative photoheterotroph, Rhodobacter sphaeroides 2.4.1, and was shown to retain
Autor:
Tomohiro, Kimura, Krishna, Vukoti, Diane L, Lynch, Dow P, Hurst, Alan, Grossfield, Michael C, Pitman, Patricia H, Reggio, Alexei A, Yeliseev, Klaus, Gawrisch
Publikováno v:
Proteins. 82(3)
The global fold of human cannabinoid type 2 (CB2 ) receptor in the agonist-bound active state in lipid bilayers was investigated by solid-state (13)C- and (15)N magic-angle spinning (MAS) NMR, in combination with chemical-shift prediction from a stru