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pro vyhledávání: '"Alexandre Avaro"'
Autor:
Juan Santiago, Alexandre Avaro
Publikováno v:
Lab on a Chip. 23:938-963
Reviewed are nucleic acid detection assays that incorporate clustered regularly interspaced short palindromic repeats (CRISPR)-based diagnostics and microfluidic devices and techniques. The review serves as a reference for researchers who wish to use
Publikováno v:
Analytical chemistry. 94(43)
The specificity of CRISPR-Cas12 assays is attractive for the detection of single nucleotide polymorphisms (SNPs) implicated in, e.g., SARS-CoV-2 variants. Such assays often employ endpoint measurements of SNP or wild type (WT) activated Cas12 trans-c
Autor:
Juan Santiago, Alexandre Avaro
Publikováno v:
Angewandte Chemie (International ed. in English). 61(45)
Michaelis-Menten kinetics is an essential model to rationalize enzyme reactions. The quantification of Michaelis-Menten parameters can be very challenging as it is sensitive to even small experimental errors. We here present a quantification of the u