Dynamics and ligand-induced conformational changes in human prolyl oligopeptidase analyzed by hydrogen/deuterium exchange mass spectrometry

Autor: Alexandra Tsirigotaki, Roos Van Elzen, Pieter Van Der Veken, Anne-Marie Lambeir, Anastassios Economou

Publikováno v: Scientific Reports, Vol 7, Iss 1, Pp 1-13 (2017)

Abstract Prolyl oligopeptidase (PREP) is conserved in many organisms across life. It is involved in numerous processes including brain function and neuropathology, that require more than its strict proteolytic role. It consists of a seven-bladed β-p

Externí odkaz: https://doaj.org/article/8b1ac353997147ac997d70c19dcf05e6

Mechanism of receptor assembly via the pleiotropic adipokine Leptin

Autor: Alexandra Tsirigotaki, Ann Dansercoer, Koen H.G. Verschueren, Iva Marković, Christoph Pollmann, Maximillian Hafer, Jan Felix, Catherine Birck, Wouter Van Putte, Dominiek Catteeuw, Jan Tavernier, J. Fernando Bazan, Jacob Piehler, Savvas N. Savvides, Kenneth Verstraete

Publikováno v: Nature Structural & Molecular Biology. 30:551-563

The adipokine Leptin activates its type I cytokine receptor (LEP-R) in the hypothalamus to regulate body weight and exerts additional pleiotropic functions in immunity, fertility, and cancer. However, the structure and mechanism of Leptin-mediated LE

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::a3315c5a9b86da7b75aec3cf441b412e
https://doi.org/10.1038/s41594-023-00941-9

Evolutionary adaptation of the folding pathway for secretability

Autor: Dries Smets, Alexandra Tsirigotaki, Jochem H. Smit, Srinath Krishnamurthy, Athina G. Portaliou, Anastassia Vorobieva, Wim Vranken, Spyridoula Karamanou, Anastassios Economou

Secretory preproteins of the Sec pathway bear signal peptides and are targeted post-translationally to cross the plasma membrane or ER through translocases. After translocation and signal peptide cleavage, mature domains fold to native states in the

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::921c199a5bbbecfced83fb928c07e6ae
https://biblio.vub.ac.be/vubir/evolutionary-adaptation-of-the-folding-pathway-for-secretability(63db8fe6-869d-4733-b221-1127bde1ea67).html

Evolution of structural dynamics in bilobed proteins

Autor: Konstantinos Tassis, Spiridoula (Lily) Karamanou, Niels Zijlstra, Martin Zacharias, Alexandra Tsirigotaki, Douglas A. Griffith, Thorben Cordes, Ruixue Xu, Giorgos Gouridis, Marijn de Boer, Anastasios Economou, Yovin Sugijo, Nikolaos Eleftheriadis, Alexander Doemling, Yusran Abdillah Muthahari

Novel biophysical tools allow the structural dynamics of proteins, and the regulation of such dynamics by binding partners, to be explored in unprecedented detail. Although this has provided critical insights into protein function, the means by which

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_________::642891632c234e02bf14d4a37b36a41c
https://doi.org/10.1101/2020.10.19.344861

Long-Lived Folding Intermediates Predominate the Targeting-Competent Secretome

Autor: Morten Beck Trelle, Georgia Orfanoudaki, Spyridoula Karamanou, Thomas J. D. Jørgensen, Jozefien De Geyter, Marina Koukaki, Athina G. Portaliou, Katerina E. Chatzi, Anastassios Economou, Marios Frantzeskos Sardis, Alexandra Tsirigotaki

Publikováno v: Tsirigotaki, A, Chatzi, K E, Koukaki, M, De Geyter, J, Portaliou, A G, Orfanoudaki, G, Sardis, M F, Trelle, M B, Jørgensen, T J D, Karamanou, S & Economou, A 2018, ' Long-Lived Folding Intermediates Predominate the Targeting-Competent Secretome ', Structure, vol. 26, no. 5, pp. 695-707.e5 . https://doi.org/10.1016/j.str.2018.03.006

Secretory preproteins carry signal peptides fused amino-terminally to mature domains. They are post-translationally targeted to cross the plasma membrane in non-folded states with the help of translocases, and fold only at their final destinations. T

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::b344c4c8225eb6cbc0df0184b6e7619d
https://pubmed.ncbi.nlm.nih.gov/29719237