Zobrazeno 1 - 10
of 27
pro vyhledávání: '"Alexandra Pokhilko"'
Autor:
Anna Flis, Aurora Piñas Fernández, Tomasz Zielinski, Virginie Mengin, Ronan Sulpice, Kevin Stratford, Alastair Hume, Alexandra Pokhilko, Megan M. Southern, Daniel D. Seaton, Harriet G. McWatters, Mark Stitt, Karen J. Halliday, Andrew J. Millar
Publikováno v:
Open Biology, Vol 5, Iss 10 (2015)
Our understanding of the complex, transcriptional feedback loops in the circadian clock mechanism has depended upon quantitative, timeseries data from disparate sources. We measure clock gene RNA profiles in Arabidopsis thaliana seedlings, grown with
Externí odkaz:
https://doaj.org/article/4ee84592a7124c0c979fa3f37779edac
Autor:
Alexandra Pokhilko, Sarah K Hodge, Kevin Stratford, Kirsten Knox, Kieron D Edwards, Adrian W Thomson, Takeshi Mizuno, Andrew J Millar
Publikováno v:
Molecular Systems Biology, Vol 6, Iss 1, Pp n/a-n/a (2010)
Circadian clocks generate 24‐h rhythms that are entrained by the day/night cycle. Clock circuits include several light inputs and interlocked feedback loops, with complex dynamics. Multiple biological components can contribute to each part of the c
Externí odkaz:
https://doaj.org/article/da4b5d28597f4e54aafce3792e073c69
Autor:
Kieron D Edwards, Ozgur E Akman, Kirsten Knox, Peter J Lumsden, Adrian W Thomson, Paul E Brown, Alexandra Pokhilko, Laszlo Kozma‐Bognar, Ferenc Nagy, David A Rand, Andrew J Millar
Publikováno v:
Molecular Systems Biology, Vol 6, Iss 1, Pp n/a-n/a (2010)
The circadian clock controls 24‐h rhythms in many biological processes, allowing appropriate timing of biological rhythms relative to dawn and dusk. Known clock circuits include multiple, interlocked feedback loops. Theory suggested that multiple l
Externí odkaz:
https://doaj.org/article/cf71725a5e234aefa766d930f55e26a4
The mTOR pathway is a global regulator of protein biosynthesis and cellular homeostasis. Understanding the differences in mTOR pathway activity between cell types is important for elucidating the role of mTOR in physiological and pathophysiological p
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::2f72a797dfdc45de3c65defe5649293f
https://doi.org/10.1101/2022.12.15.505685
https://doi.org/10.1101/2022.12.15.505685
Autor:
Caleb Webber, Colin J. Akerman, Sarah E. Newey, Viola Volpato, Michael B. Clark, Rory Bowden, Emma S. Whiteley, Adam E. Handel, M. Zameel Cader, Fabiola Curion, Alexandra Pokhilko, Sunniva Bostrand
Publikováno v:
Genome Research
Genome Res
Genome Res
Single-cell RNA sequencing (scRNA-seq) is a widely used method for identifying cell types and trajectories in biologically heterogeneous samples, but it is limited in its detection and quantification of lowly expressed genes. This results in missing
Publikováno v:
Zhao, J, Pokhilko, A, Ebenhöh, O, Rosser, S J & Colloms, S D 2019, ' A single-input binary counting module based on serine integrase site-specific recombination ', Nucleic Acids Research, vol. 47, no. 9, pp. 4896-4909 . https://doi.org/10.1093/nar/gkz245
Nucleic Acids Research
Nucleic Acids Research
A device that counts and records the number of events experienced by an individual cell could have many uses in experimental biology and biotechnology. Here, we report a DNA-based ‘latch’ that switches between two states upon each exposure to a r
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::911c14c9e98418b5bedb87f9bf57d165
https://hdl.handle.net/20.500.11820/47961001-3d3d-4626-b86c-e8f5a534c618
https://hdl.handle.net/20.500.11820/47961001-3d3d-4626-b86c-e8f5a534c618
Site-specific recombination promoted by serine integrases can be used for ordered assembly of DNA fragments into larger arrays. When a plasmid vector is included in the assembly, the circular product DNA molecules can transformE. colicells. A conveni
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::074ad91183d150e28ba5dab4fb7e97b5
https://doi.org/10.1101/433441
https://doi.org/10.1101/433441
Autor:
Alexandra Pokhilko
Publikováno v:
BMC Systems Biology, Vol 11, Iss 1, Pp 1-10 (2017)
BMC Systems Biology
BMC Systems Biology
Background E. coli can be used as bacterial cell factories for production of biofuels and other useful compounds. The efficient production of the desired products requires careful monitoring of growth conditions and the optimization of metabolic flux
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::449226f158711b2ac1e568f9adc7eac5
https://eprints.gla.ac.uk/151437/1/151437.pdf
https://eprints.gla.ac.uk/151437/1/151437.pdf
Publikováno v:
Journal of the Royal Society Interface
Serine integrases catalyse site-specific recombination to integrate and excise bacteriophage genomes into and out of their host's genome. These enzymes exhibit remarkable directionality; in the presence of the integrase alone, recombination between a
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::f075fe141da66974e73a34dcfe75d8cf
https://eprints.gla.ac.uk/132611/1/132611.pdf
https://eprints.gla.ac.uk/132611/1/132611.pdf
Autor:
Andrew J. Millar, Rajnish Khanna, Don R. Maszle, Jason Ramos, Hans E. Holtan, Alexandra Pokhilko
Publikováno v:
Journal of Theoretical Biology
Journal of Theoretical Biology, Elsevier, 2011, 270 (1), pp.31. ⟨10.1016/j.jtbi.2010.11.021⟩
Pokhilko, A, Ramos, J A, Holtan, H, Maszle, D R, Khanna, R & Millar, A J 2011, ' Ubiquitin ligase switch in plant photomorphogenesis : A hypothesis ', Journal of Theoretical Biology, vol. 270, no. 1, pp. 31-41 . https://doi.org/10.1016/j.jtbi.2010.11.021
Journal of Theoretical Biology, Elsevier, 2011, 270 (1), pp.31. ⟨10.1016/j.jtbi.2010.11.021⟩
Pokhilko, A, Ramos, J A, Holtan, H, Maszle, D R, Khanna, R & Millar, A J 2011, ' Ubiquitin ligase switch in plant photomorphogenesis : A hypothesis ', Journal of Theoretical Biology, vol. 270, no. 1, pp. 31-41 . https://doi.org/10.1016/j.jtbi.2010.11.021
The E3 ubiquitin ligase COP1 (CONSTITUTIVE PHOTOMORPHOGENIC1) plays a key role in the repression of the plant photomorphogenic development in darkness. In the presence of light, COP1 is inactivated by a mechanism which is not completely understood. T