Zobrazeno 1 - 10
of 12
pro vyhledávání: '"Alexandra Lerchner"'
Autor:
Rajiv Datar, Carlo Marchetti, Benjamin J Swartzwelter, Charles A. Dinarello, Carl K. Edwards, Uli Binder, Martin Schlapschy, Arne Skerra, Dennis M. de Graaf, Nicholas E. Powers, Alexandra Lerchner
Publikováno v:
J Biol Chem
Journal of Biological Chemistry, 295, 3, pp. 868-882
Journal of Biological Chemistry, 295, 868-882
Journal of Biological Chemistry, 295, 3, pp. 868-882
Journal of Biological Chemistry, 295, 868-882
Interleukin-1 (IL-1) is a key mediator of inflammation and immunity. Naturally-occurring IL-1 receptor antagonist (IL-1Ra) binds and blocks the IL-1 receptor-1 (IL-1R1), preventing signaling. Anakinra, a recombinant form of IL-1Ra, is used to treat a
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::dc4c17b1368a9c115cafec49dfdb8696
https://doi.org/10.1016/s0021-9258(17)49941-8
https://doi.org/10.1016/s0021-9258(17)49941-8
Publikováno v:
Biotechnology and Applied Biochemistry. 63:616-624
The l-alanine dehydrogenase of Bacillus subtilis (BasAlaDH), which is strictly dependent on NADH as redox cofactor, efficiently catalyzes the reductive amination of pyruvate to l-alanine using ammonia as amino group donor. To enable application of Ba
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::bc5ebc075fee9a9ec69a180ffe75174f
https://doi.org/10.1002/bab.1414
https://doi.org/10.1002/bab.1414
Publikováno v:
ChemCatChem. 5:3374-3383
A matching dehydrogenase and transaminase pair was engineered with regard to substrate recognition, catalytic activity, and cofactor specificity with the final goal to convert the bicyclic dialcohol isosorbide into a diamine by a multistep biocatalyt
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::f68f2fb144a9d5b010009086a924a427
https://doi.org/10.1002/cctc.201300284
https://doi.org/10.1002/cctc.201300284
Publikováno v:
Biotechnology and Bioengineering. 110:2803-2814
The NADP+-dependent alcohol dehydrogenase from Ralstonia sp. (RasADH) belongs to the protein superfamily of short-chain dehydrogenases/reductases (SDRs). As an enzyme that accepts different types of substrates—including bulky–bulky as well as sma
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::bef0dcea8a16f9f84416cf725ff54191
https://doi.org/10.1002/bit.24956
https://doi.org/10.1002/bit.24956
Publikováno v:
Proteins: Structure, Function, and Bioinformatics. 81:774-787
Apart from their crucial role in metabolism, pyridoxal 5'-phosphate (PLP)-dependent aminotransferases (ATs) constitute a class of enzymes with increasing application in industrial biotechnology. To provide better insight into the structure-function r
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::9ae676911687f43aa1dafc3963cfdc1f
https://doi.org/10.1002/prot.24233
https://doi.org/10.1002/prot.24233
Publikováno v:
Scopus-Elsevier
To facilitate biocatalytic conversion of the biotechnologically accessible dicyclic dialcohol isosorbide into its industrially relevant diamines, we have designed a fusion protein between two homo-oligomeric enzymes: the levodione reductase (LR) from
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::9f25bba21433d16da0063e037a860c43
https://doi.org/10.1093/protein/gzw039
https://doi.org/10.1093/protein/gzw039
Publikováno v:
Biotechnology and applied biochemistry. 63(5)
The l-alanine dehydrogenase of Bacillus subtilis (BasAlaDH), which is strictly dependent on NADH as redox cofactor, efficiently catalyzes the reductive amination of pyruvate to l-alanine using ammonia as amino group donor. To enable application of Ba
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=pmid________::c4fc5ddebd3617513f0a25818149a3e2
https://pubmed.ncbi.nlm.nih.gov/26202482
https://pubmed.ncbi.nlm.nih.gov/26202482
Autor:
Alexandra Lerchner, Ines Schäffner, Regina Schöps, Renate Ulbrich-Hofmann, Johanna Mansfeld, Helge K. Beer
Publikováno v:
Biochimica et Biophysica Acta (BBA) - Molecular and Cell Biology of Lipids. 1737:94-101
The genes of two phospholipase D (PLD) isoenzymes, PLD1 and PLD2, from poppy seedlings (2829 and 2828 bp) were completely sequenced. The two genes have 96.9% identity in the encoding region and can be assigned to the α-type of plant PLDs. The corres
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::7927f5ed8cb5ad50e68ed01cc1f4ca7b
https://doi.org/10.1016/j.bbalip.2005.09.010
https://doi.org/10.1016/j.bbalip.2005.09.010
Publikováno v:
Biotechnology Letters. 27:535-544
Phospholipase D (PLD) from plants or microorganisms is used as biocatalyst in the transformation of phospholipids and phospholipid analogs in both laboratory and industrial scale. In recent years the elucidation of the primary structure of many PLDs
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::b0bd217b35da1010e9db8a637c88bb78
https://doi.org/10.1007/s10529-005-3251-2
https://doi.org/10.1007/s10529-005-3251-2
Autor:
Angelika Schierhorn, Renate Ulbrich-Hofmann, Alexandra Lerchner, Hina Younus, Karl-Peter Rücknagel, Regina Schöps, M. Saleemuddin
Publikováno v:
Journal of Protein Chemistry. 22:499-508
A recombinant phospholipase D from white cabbage (PLD2) composed of 812 amino acid residues was studied by site-directed mutagenesis and limited proteolysis to obtain first information on its tertiary structure. Limited proteolysis by thermolysin res
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::9150cbc266efb5c0912fdaa85434971f
https://doi.org/10.1023/b:jopc.0000005498.13074.72
https://doi.org/10.1023/b:jopc.0000005498.13074.72