Zobrazeno 1 - 10
of 11
pro vyhledávání: '"Alexander Zhelkovsky"'
Autor:
Robert J Bauer, Alexander Zhelkovsky, Katharina Bilotti, Laura E Crowell, Thomas C Evans, Larry A McReynolds, Gregory J S Lohman
Publikováno v:
PLoS ONE, Vol 12, Iss 12, p e0190062 (2017)
DNA ligases catalyze the repair of phosphate backbone breaks in DNA, acting with highest activity on breaks in one strand of duplex DNA. Some DNA ligases have also been observed to ligate two DNA fragments with short complementary overhangs or blunt-
Externí odkaz:
https://doaj.org/article/4c9855df9df64095983c0ca3fb0489fe
Autor:
Claire Moore, Alexander Zhelkovsky, Stephen Buratowski, Oliver J. Rando, Minkyu Kim, Lidia Vasiljeva
Publikováno v:
Molecular Cell. 24(5):723-734
Transcription termination at mRNA genes is linked to polyadenylation. Cleavage at the poly(A) site generates an entry point for the Rat1/Xrn2 exonuclease, which degrades the downstream transcript to promote termination. Small nucleolar RNAs (snoRNAs)
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::b534d43938e972bd6c25fd729855913d
Autor:
Torben Heick Jensen, Yoko Tacahashi, Tommy Nasser, Xiaoyuan He, Claire Moore, Horst Domdey, Ulrike Sterzer, Alexander Zhelkovsky
Publikováno v:
RNA. 12:435-445
The cleavage/polyadenylation factor (CPF) of Saccharomyces cerevisiae is thought to provide the catalytic activities of the mRNA 3′-end processing machinery, which include endonucleolytic cleavage at the poly(A) site, followed by synthesis of an ad
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::fe2f137d3e11b6ccb1d8d012af5066db
https://doi.org/10.1261/rna.2267606
https://doi.org/10.1261/rna.2267606
Publikováno v:
RNA. 10:558-564
The eukaryotic poly(A) polymerase (PAP) is responsible for the posttranscriptional extension of mRNA 3′ ends by the addition of a poly(A) tract. The recently published three-dimensional structures of yeast and bovine PAPs have made a more directed
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::791d0225d4c9f78b14ffc085954dbbe1
https://doi.org/10.1261/rna.5238704
https://doi.org/10.1261/rna.5238704
Publikováno v:
Journal of Biological Chemistry. 270:26715-26720
We have constructed deletions in the nonconserved regions at the amino and carboxyl ends of the poly(A) polymerase (PAP) of Saccharomyces cerevisiae and examined the effects of these truncations on function of the enzyme. PAP synthesizes a poly(A) ta
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::55b7f3277b72bd995cc0b4c12ef0e517
https://doi.org/10.1074/jbc.270.44.26715
https://doi.org/10.1074/jbc.270.44.26715
Synthesis of the poly(A) tail of mRNA in Saccharomyces cerevisiae requires recruitment of the polymerase Pap1 to the 3′ end of cleaved pre-mRNA. This is made possible by the tethering of Pap1 to the Cleavage/Polyadenylation Factor (CPF) by Fip1. We
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::fa8f3597172e4d0f52e43a3d5b1ea46a
https://europepmc.org/articles/PMC3062176/
https://europepmc.org/articles/PMC3062176/
Fip1 is an essential component of the Saccharomyces cerevisiae polyadenylation machinery and the only protein known to interact directly with poly(A) polymerase (Pap1). Its association with Pap1 inhibits the extension of an oligo(A) primer by limitin
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::525f4059fed4bbbbb127dde12003ff8f
https://europepmc.org/articles/PMC86804/
https://europepmc.org/articles/PMC86804/
Publikováno v:
Science (New York, N.Y.). 289(5483)
Polyadenylate [poly(A)] polymerase (PAP) catalyzes the addition of a polyadenosine tail to almost all eukaryotic messenger RNAs (mRNAs). The crystal structure of the PAP from Saccharomyces cerevisiae (Pap1) has been solved to 2.6 angstroms, both alon
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::e4430fb69723594cb20e98918b008276
https://pubmed.ncbi.nlm.nih.gov/10958780
https://pubmed.ncbi.nlm.nih.gov/10958780
Publikováno v:
Molecular and cellular biology. 18(10)
Cleavage and polyadenylation of the 3′ end of eukaryotic precursor mRNA is a modification essential for proper mRNA utilization. The primary function of poly(A) polymerase (PAP) in this processing reaction is to add poly(A) tails to the cleaved pre
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::6938d7b81f49ae69ab2b4d69ccd191fc
https://pubmed.ncbi.nlm.nih.gov/9742111
https://pubmed.ncbi.nlm.nih.gov/9742111
Publikováno v:
Biochemistry. 34(5)
Purified yeast poly(A) polymerase (PAP) was used to produce monoclonal antibodies which recognize the enzyme in immunoblots. Epitope mapping using truncated forms of PAP and cyanogen bromide cleavage products revealed two classes of antibodies. One c
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::adc8915f38d587e058c74a25d9afc615
https://pubmed.ncbi.nlm.nih.gov/7849035
https://pubmed.ncbi.nlm.nih.gov/7849035