Illumina But With Nanopore: Sequencing Illumina libraries at high accuracy on the ONT MinION using R2C2

Autor: Russell Corbett-Detig, Kayla D. Schimke, Shelbi L Russell, Dori Z. Q. Deng, Xuan Zhang, Matthew Adams, Christopher Vollmers, Alexander Zee, Robert J. Schmitz

High-throughput short-read sequencing has taken on a central role in research and diagnostics. Literally hundreds of different assays exist today to take advantage of Illumina short-read sequencers, the predominant short-read sequencing technology av

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_________::00fe6256f25c98ea5b0029cd43901b4e
https://doi.org/10.1101/2021.10.30.466545

A single-cell transcriptomic atlas characterizes ageing tissues in the mouse

Autor: Nicholas Schaum, Ashley Maynard, Kenneth I. Weinberg, Ishita Bansal, Annie Lo, Christin S. Kuo, Hamid Ebadi, Norma Neff, Bryan D. Merrill, Gunsagar S. Gulati, Michelle B. Chen, Nathalie Khoury, Song E. Lee, Martin J. Zhang, Michael N. Wosczyna, Linda J. van Weele, Lakshmi P. Yerra, James Zou, Matt Fish, Michael F. Clarke, Antoine de Morrée, Lucas M. Waldburger, Kyle J. Travaglini, Maria F. Lugo-Fagundo, Yan Hang, Rasika Patkar, Kerwyn Casey Huang, Weng Chuan Peng, Wan Jin Lu, Astrid Gillich, Andrew May, Aaron Demers, Tony Wyss-Coray, Benoit Lehallier, William Kong, Douglas Brownfield, Robert C. Jones, Katharine M. Ng, Ankit S. Baghel, Patricia K. Nguyen, Rafael Gòmez-Sjöberg, Katherine S. Pollard, Ling Liu, Kevin S. Kao, Róbert Pálovics, Taichi Isobe, Chunyu Zhao, Roel Nusse, Eric J. Rulifson, Maya E. Kumar, Bruce Wang, Philip A. Beachy, Tessa Divita, Ross J. Metzger, Cristina M. Tato, Thomas A. Rando, Marina McKay, Hui Zhang, Oliver Hahn, Jinyi Xiang, Jane Antony, Aaron McGeever, Daniel Staehli, Macy E. Zardeneta, Tal Iram, Olivia Leventhal, Qingyun Li, Angela Oliveira Pisco, Lolita Penland, Krissie Tellez, Marco Mignardi, Brian Yu, Shaheen S. Sikandar, Lincoln Harris, Nicole Almanzar, Corey Cain, Geraldine Genetiano, Foad Green, Davis P. Lee, Carolina Tropini, Laughing Bear Torrez Dulgeroff, Rahul Sinha, Zhen Qi, Stephen R. Quake, Charles Chan, Irving L. Weissman, Sean M. Wu, Jim Karkanias, Ahmad N. Nabhan, Andreas Keller, Margaret Tsui, Alexander Zee, Guruswamy Karnam, Michael S. Haney, Haley du Bois, Robert Puccinelli, Ben A. Barres, Justin L. Sonnenburg, F. Hernan Espinoza, Fabio Zanini, Qiang Gan, Joseph Noh, Lu Zhou, Isaac Bakerman, Aaron M. Kershner, Mark A. Krasnow, Kubilay Demir, Feather Ives, Benson M. George, Guang Li, Dullei Min, Justin Youngyunpipatkul, Mu He, Rene V. Sit, Bernhard M. Kiss, Stephanie D. Conley, Seung K. Kim, Weilun Tan, Soso Xue, M. Windy McNerney, Andrew C. Yang, Kevin A. Yamauchi, Albin Huang, Joe M. Segal, Krzysztof Szade, Michelle Tan, Biter Bilen, Fan Zhang, Spyros Darmanis, Shayan Hosseinzadeh, Xueying Gu, Jonathan K. Lam, Anoop Manjunath, Daniela Berdnik

Publikováno v: Nature

Aging is characterized by a progressive loss of physiological integrity, leading to impaired function and increased vulnerability to death(1). Despite rapid advances over recent years, many of the molecular and cellular processes which underlie progr

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::a1c3489ceeac8cf1e8dc138771ff6428
https://ruj.uj.edu.pl/xmlui/handle/item/279563

Therapy-Induced Evolution of Human Lung Cancer Revealed by Single-Cell RNA Sequencing

Autor: Erin L. Schenk, Thierry Jahan, Trever G. Bivona, Anshal Gupta, Rafael Gomez-Sjoberg, Norma Neff, Nicholas J. Thomas, Franziska Haderk, Bianca Bacaltos, Spyros Darmanis, Pallav K. Kolli, Hien Do, Johannes R. Kratz, D. Lucas Kerr, Robert C. Doebele, Khyati N. Shah, Julia K Rotow, Mayra Gonzalez, Matthew A. Gubens, Yaron Gesthalter, Caroline E. McCoach, Kirk D. Jones, Eric J. Seeley, Anatoly Urisman, Ashley Maynard, Daniel D. Le, Michelle Tan, Philippe Gui, Lisa Tan, Weilun Tan, Tasha Lea, Sourav Bandyopadhyay, David M. Naeger, Lincoln Harris, Collin M. Blakely, Lauren Cech, Alexander Zee, David M. Jablons, Rene Sit, Wei Wu, Jonathan S. Weissman, Kevin A. Yamauchi, Elizabeth A. Yu

Publikováno v: Cell

Summary Lung cancer, the leading cause of cancer mortality, exhibits heterogeneity that enables adaptability, limits therapeutic success, and remains incompletely understood. Single-cell RNA sequencing (scRNA-seq) of metastatic lung cancer was perfor

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::794e39225d45574e5c6f24ca9d417b48
https://pubmed.ncbi.nlm.nih.gov/32822576