Zobrazeno 1 - 10
of 147
pro vyhledávání: '"Alexander S. Zasedatelev"'
Autor:
Dmitry A. Khodakov, Natalia V. Zakharova, Dmitry A. Gryadunov, Felix P. Filatov, Alexander S. Zasedatelev, Vladimir M. Mikhailovich
Publikováno v:
BioTechniques, Vol 44, Iss 2, Pp 241-248 (2008)
We describe a novel microarray-based approach for simultaneous identification and quantification of human immunodeficiency virus type 1 (HIV-1) and hepatitis B and C viruses (HBV and HCV) in donor plasma specimens. The method is based on multiplex re
Externí odkaz:
https://doaj.org/article/95b3027a967e43b98d547a5ea1f001ed
Autor:
Andrey L. Mikheikin, Sergey A Surzhikov, Olga A. Zasedateleva, Vadim A. Vasiliskov, Sergey V. Pan'kov, Irina V. Grechishnikova, Lev L. Kisselev, Alexander S. Zasedatelev
Publikováno v:
BioTechniques, Vol 44, Iss 1, Pp 77-83 (2008)
To analyze RNA interactions with RNA binding molecules an RNA microchip containing immobilized oligoribonucleotides with protective groups [t-butyldimethylsilyl (tBDMS)] at 2′-O- positions was developed. The oligonucleotides were immobilized within
Externí odkaz:
https://doaj.org/article/7a75dda097d24a06a9dd97bb8002b2fd
Publikováno v:
BioTechniques, Vol 42, Iss 5, Pp 590-594 (2007)
Externí odkaz:
https://doaj.org/article/90699525a6da465fbb56ecc650becfbe
Autor:
R. A. Miftakhov, Alexander V. Chudinov, Alexander S. Zasedatelev, Sergey A. Lapa, E. S. Klochikhina
Publikováno v:
Russian Journal of Bioorganic Chemistry. 47:1122-1125
A variant of multiplex PCR on a chip with direct detection of immobilized primer elongation has been developed. Detection is performed by determining the fluorescence signal of labeled dUTPs incorporated in the growing chain. This approach creates an
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::377f6307e8ab9d81cf2638ce33c1f47d
https://doi.org/10.1134/s1068162021050290
https://doi.org/10.1134/s1068162021050290
Autor:
Viktoriya E. Kuznetsova, Valeriy E. Shershov, Teimur O. Guseinov, Rinat A. Miftakhov, Pavel N. Solyev, Roman A. Novikov, Anna I. Levashova, Alexander S. Zasedatelev, Sergey A. Lapa, Alexander V. Chudinov
Publikováno v:
Bioorganic chemistry. 131
New applications of palladium-catalyzed Sonogashira-type cross-coupling reaction between C5-halogenated 2'-deoxycytidine-5'-monophosphate and novel cyanine dyes with a terminal alkyne group have been developed. The present methodology allows to synth
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::35ab9dad5f5e0ef748d707a4d95e3ad8
https://pubmed.ncbi.nlm.nih.gov/36528924
https://pubmed.ncbi.nlm.nih.gov/36528924
Autor:
S A Blagodatskikh, V. E. Shershov, Alexander S. Zasedatelev, Sergey A. Lapa, R. A. Miftakhov, E. S. Klochikhina, Alexander V. Chudinov, Yu I Ammur
Publikováno v:
Молекулярная биология. 55:944-955
A prototype of a system for the detection of infectious human pneumonia pathogens based on multiplex solid-phase reverse transcription PCR (RT-PCR) was developed. Primers were designed to identify the DNA of six bacterial pneumonia pathogen strains,
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::50b28d6b675b4ec70331ca06f04d59e4
https://doi.org/10.31857/s0026898421050062
https://doi.org/10.31857/s0026898421050062
Autor:
V. E. Kuznetsova, Alexander V. Chudinov, A. S. Pavlov, T. O. Guseinov, Alexander S. Zasedatelev, Sergey A. Lapa, V. E. Shershov
Publikováno v:
Russian Journal of Bioorganic Chemistry. 46:557-562
Particular features of the simultaneous incorporation into the growing DNA chain of modified Cy5-deoxyuridines (Cy5-dU) and deoxycytidines (Cy5-dC) and Taq DNA polymerase in the process of PCR amplification were studied. The studies were carried out
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::a3d459d7fccb8a123ee3a804be6c5942
https://doi.org/10.1134/s1068162020040111
https://doi.org/10.1134/s1068162020040111
Autor:
George S. Krasnov, O. S. Volkova, S. P. Radko, V. E. Shershov, Alexander S. Zasedatelev, Sergey A. Lapa, V. E. Kuznetsova, Alexander V. Chudinov
Publikováno v:
Russian Journal of Bioorganic Chemistry. 46:551-556
A method of thermal dissociation of DNA–protein conjugates was developed for the selection of aptamers to human alpha-fetoprotein. The method is based on the identification of the DNA oligonucleotides from the combinatorial DNA libraries with the h
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::c3feddd3f0bac53b82a03b8ac357a6aa
https://doi.org/10.1134/s106816202004010x
https://doi.org/10.1134/s106816202004010x
Autor:
R. A. Miftahov, Edward N. Timofeev, V. E. Shershov, V. E. Kuznetsova, Alexander V. Chudinov, Alexander S. Zasedatelev, Sergey A. Lapa
Publikováno v:
Russian Journal of Bioorganic Chemistry. 46:264-268
For emulsion PCR amplification of oligonucleotide combinatorial libraries the conditions for the stable generation of water-in-oil emulsions with drops of optimal size were found, which included the use of 3% ABIL EM 180 silicone emulsifier in the pr
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::90bf231e33dff5b25b23488732ca34c4
https://doi.org/10.1134/s1068162020020120
https://doi.org/10.1134/s1068162020020120
Autor:
Irina V. Grechishnikova, Alexander S. Zasedatelev, Sergey A. Lapa, V. E. Kuznetsova, Alexander V. Chudinov, V. E. Shershov, Andrey A. Stomahin, Edward N. Timofeev, R. A. Miftakhov
Publikováno v:
Mendeleev Communications. 31:70-72
A novel electroneutral rigid meso-sulfophenyloxy substituted heptamethine dye was synthesized in six steps. Selective derivatization of one sulfonamide group with octanedioic acid introduced the carboxy end group attached through the hexamethylene li
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::03c690f414b5b615c6dcc2301d7f6f89
https://doi.org/10.1016/j.mencom.2021.01.021
https://doi.org/10.1016/j.mencom.2021.01.021