Zobrazeno 1 - 10
of 159
pro vyhledávání: '"Alexander S. Zasedatelev"'
Autor:
Viktoriya E. Kuznetsova, Valeriy E. Shershov, Teimur O. Guseinov, Rinat A. Miftakhov, Pavel N. Solyev, Roman A. Novikov, Anna I. Levashova, Alexander S. Zasedatelev, Sergey A. Lapa, Alexander V. Chudinov
Publikováno v:
Bioorganic chemistry. 131
New applications of palladium-catalyzed Sonogashira-type cross-coupling reaction between C5-halogenated 2'-deoxycytidine-5'-monophosphate and novel cyanine dyes with a terminal alkyne group have been developed. The present methodology allows to synth
Autor:
S A Blagodatskikh, V. E. Shershov, Alexander S. Zasedatelev, Sergey A. Lapa, R. A. Miftakhov, E. S. Klochikhina, Alexander V. Chudinov, Yu I Ammur
Publikováno v:
Молекулярная биология. 55:944-955
A prototype of a system for the detection of infectious human pneumonia pathogens based on multiplex solid-phase reverse transcription PCR (RT-PCR) was developed. Primers were designed to identify the DNA of six bacterial pneumonia pathogen strains,
Autor:
V. E. Kuznetsova, Alexander V. Chudinov, A. S. Pavlov, T. O. Guseinov, Alexander S. Zasedatelev, Sergey A. Lapa, V. E. Shershov
Publikováno v:
Russian Journal of Bioorganic Chemistry. 46:557-562
Particular features of the simultaneous incorporation into the growing DNA chain of modified Cy5-deoxyuridines (Cy5-dU) and deoxycytidines (Cy5-dC) and Taq DNA polymerase in the process of PCR amplification were studied. The studies were carried out
Autor:
George S. Krasnov, O. S. Volkova, S. P. Radko, V. E. Shershov, Alexander S. Zasedatelev, Sergey A. Lapa, V. E. Kuznetsova, Alexander V. Chudinov
Publikováno v:
Russian Journal of Bioorganic Chemistry. 46:551-556
A method of thermal dissociation of DNA–protein conjugates was developed for the selection of aptamers to human alpha-fetoprotein. The method is based on the identification of the DNA oligonucleotides from the combinatorial DNA libraries with the h
Autor:
R. A. Miftahov, Edward N. Timofeev, V. E. Shershov, V. E. Kuznetsova, Alexander V. Chudinov, Alexander S. Zasedatelev, Sergey A. Lapa
Publikováno v:
Russian Journal of Bioorganic Chemistry. 46:264-268
For emulsion PCR amplification of oligonucleotide combinatorial libraries the conditions for the stable generation of water-in-oil emulsions with drops of optimal size were found, which included the use of 3% ABIL EM 180 silicone emulsifier in the pr
Autor:
Dmitry A. Khodakov, Natalia V. Zakharova, Dmitry A. Gryadunov, Felix P. Filatov, Alexander S. Zasedatelev, Vladimir M. Mikhailovich
Publikováno v:
BioTechniques, Vol 44, Iss 2, Pp 241-248 (2008)
We describe a novel microarray-based approach for simultaneous identification and quantification of human immunodeficiency virus type 1 (HIV-1) and hepatitis B and C viruses (HBV and HCV) in donor plasma specimens. The method is based on multiplex re
Externí odkaz:
https://doaj.org/article/95b3027a967e43b98d547a5ea1f001ed
Autor:
Andrey L. Mikheikin, Sergey A Surzhikov, Olga A. Zasedateleva, Vadim A. Vasiliskov, Sergey V. Pan'kov, Irina V. Grechishnikova, Lev L. Kisselev, Alexander S. Zasedatelev
Publikováno v:
BioTechniques, Vol 44, Iss 1, Pp 77-83 (2008)
To analyze RNA interactions with RNA binding molecules an RNA microchip containing immobilized oligoribonucleotides with protective groups [t-butyldimethylsilyl (tBDMS)] at 2′-O- positions was developed. The oligonucleotides were immobilized within
Externí odkaz:
https://doaj.org/article/7a75dda097d24a06a9dd97bb8002b2fd
Autor:
Irina V. Grechishnikova, Alexander S. Zasedatelev, Sergey A. Lapa, V. E. Kuznetsova, Alexander V. Chudinov, V. E. Shershov, Andrey A. Stomahin, Edward N. Timofeev, R. A. Miftakhov
Publikováno v:
Mendeleev Communications. 31:70-72
A novel electroneutral rigid meso-sulfophenyloxy substituted heptamethine dye was synthesized in six steps. Selective derivatization of one sulfonamide group with octanedioic acid introduced the carboxy end group attached through the hexamethylene li
Publikováno v:
BioTechniques, Vol 42, Iss 5, Pp 590-594 (2007)
Externí odkaz:
https://doaj.org/article/90699525a6da465fbb56ecc650becfbe
Autor:
O. S. Volkova, Alexander V. Chudinov, A. S. Pavlov, V. E. Kuznetsova, Alexander S. Zasedatelev, Sergey A. Lapa, V. E. Shershov
Publikováno v:
Russian Journal of Bioorganic Chemistry. 46:856-858
The substrate efficiency of modified derivatives of 2'-deoxyuridine-(mod-dUTP) and 2'-deoxycytidine-5'-triphosphates (mod-dCTP) was studied in the regime of simultaneous incorporation using the primer elongation reaction (PEX) and PCR with complete r