Zobrazeno 1 - 10
of 86
pro vyhledávání: '"Alexander S Mishin"'
Autor:
Louisa Gonzalez Somermeyer, Aubin Fleiss, Alexander S Mishin, Nina G Bozhanova, Anna A Igolkina, Jens Meiler, Maria-Elisenda Alaball Pujol, Ekaterina V Putintseva, Karen S Sarkisyan, Fyodor A Kondrashov
Publikováno v:
eLife, Vol 11 (2022)
Studies of protein fitness landscapes reveal biophysical constraints guiding protein evolution and empower prediction of functional proteins. However, generalisation of these findings is limited due to scarceness of systematic data on fitness landsca
Externí odkaz:
https://doaj.org/article/ed18ecf491af457191a65695ab4e463d
Autor:
Nina G Bozhanova, Joel M Harp, Brian J Bender, Alexey S Gavrikov, Dmitry A Gorbachev, Mikhail S Baranov, Christina B Mercado, Xuan Zhang, Konstantin A Lukyanov, Alexander S Mishin, Jens Meiler
Publikováno v:
PLoS Computational Biology, Vol 17, Iss 11, p e1009555 (2021)
The use of unnatural fluorogenic molecules widely expands the pallet of available genetically encoded fluorescent imaging tools through the design of fluorogen activating proteins (FAPs). While there is already a handful of such probes available, eac
Externí odkaz:
https://doaj.org/article/db709dc63ab7480d90a2d7fc01f83e8c
Autor:
Victoria O Pokusaeva, Dinara R Usmanova, Ekaterina V Putintseva, Lorena Espinar, Karen S Sarkisyan, Alexander S Mishin, Natalya S Bogatyreva, Dmitry N Ivankov, Arseniy V Akopyan, Sergey Ya Avvakumov, Inna S Povolotskaya, Guillaume J Filion, Lucas B Carey, Fyodor A Kondrashov
Publikováno v:
PLoS Genetics, Vol 15, Iss 4, p e1008079 (2019)
Characterizing the fitness landscape, a representation of fitness for a large set of genotypes, is key to understanding how genetic information is interpreted to create functional organisms. Here we determined the evolutionarily-relevant segment of t
Externí odkaz:
https://doaj.org/article/e66bf1d0a4ec429799bdc20106b09434
Autor:
Nadya V Pletneva, Vladimir Z Pletnev, Karen S Sarkisyan, Dmitry A Gorbachev, Evgeny S Egorov, Alexander S Mishin, Konstantin A Lukyanov, Zbigniew Dauter, Sergei Pletnev
Publikováno v:
PLoS ONE, Vol 10, Iss 12, p e0145740 (2015)
Phototoxic fluorescent proteins represent a sparse group of genetically encoded photosensitizers that could be used for precise light-induced inactivation of target proteins, DNA damage, and cell killing. Only two such GFP-based fluorescent proteins
Externí odkaz:
https://doaj.org/article/e36b7701a2794df4983a7c5bab7f0b65
Autor:
Karen S Sarkisyan, Olga A Zlobovskaya, Dmitry A Gorbachev, Nina G Bozhanova, George V Sharonov, Dmitriy B Staroverov, Evgeny S Egorov, Anastasia V Ryabova, Kyril M Solntsev, Alexander S Mishin, Konstantin A Lukyanov
Publikováno v:
PLoS ONE, Vol 10, Iss 12, p e0145287 (2015)
Genetically encoded photosensitizers, proteins that produce reactive oxygen species when illuminated with visible light, are increasingly used as optogenetic tools. Their applications range from ablation of specific cell populations to precise optica
Externí odkaz:
https://doaj.org/article/4be45a6ed02044b89daa9fdc7e3683da
Autor:
Bobin George Abraham, Karen S Sarkisyan, Alexander S Mishin, Ville Santala, Nikolai V Tkachenko, Matti Karp
Publikováno v:
PLoS ONE, Vol 10, Iss 8, p e0134436 (2015)
Fluorescence Resonance Energy Transfer (FRET) using fluorescent protein variants is widely used to study biochemical processes in living cells. FRET detection by fluorescence lifetime measurements is the most direct and robust method to measure FRET.
Externí odkaz:
https://doaj.org/article/e16d60ab5e0f49e8941539f628867627
Autor:
Marina V. Goncharuk, Nadezhda S. Baleeva, Dmitry E. Nolde, Alexey S. Gavrikov, Alexey V. Mishin, Alexander S. Mishin, Andrey Y. Sosorev, Alexander S. Arseniev, Sergey A. Goncharuk, Valentin I. Borshchevskiy, Roman G. Efremov, Konstantin S. Mineev, Mikhail S. Baranov
Publikováno v:
Communications Biology, Vol 5, Iss 1, Pp 1-10 (2022)
Structural analyses guide the rational design of mutant fluorogen-activating proteins with enhanced fluorescence.
Externí odkaz:
https://doaj.org/article/9f5e4001803e45c99f3b5822fd1097f3
Autor:
Maxim M. Perfilov, Elvira R. Zaitseva, Nadezhda S. Baleeva, Vadim S. Kublitski, Alexander Yu. Smirnov, Yulia A. Bogdanova, Svetlana A. Krasnova, Ivan N. Myasnyanko, Alexander S. Mishin, Mikhail S. Baranov
Publikováno v:
International Journal of Molecular Sciences, Vol 24, Iss 12, p 9923 (2023)
In this work, we have shown that the introduction of a trifluoromethyl group into the me-ta-position of arylidene imidazolones (GFP chromophore core) leads to a dramatic increase in their fluorescence in nonpolar and aprotic media. The presence of a
Externí odkaz:
https://doaj.org/article/f0f6d8f1f8ee42ea942bd2493057c6fd
Autor:
Yulia A. Bogdanova, Elvira R. Zaitseva, Alexander Yu. Smirnov, Nadezhda S. Baleeva, Alexey S. Gavrikov, Ivan N. Myasnyanko, Sergey A. Goncharuk, Erik F. Kot, Konstantin S. Mineev, Alexander S. Mishin, Mikhail S. Baranov
Publikováno v:
International Journal of Molecular Sciences, Vol 24, Iss 9, p 7958 (2023)
In this work, we showed that the well-known NanoLuc luciferase can act as a fluorogen activating protein for various arylidene-imidazolones structurally similar to the Kaede protein chromophore. We showed that such compounds can be used as fluorescen
Externí odkaz:
https://doaj.org/article/b130c468b8374241b16288573c4516b1
Autor:
Kseniia A. Palkina, Anastasia V. Balakireva, Olga A. Belozerova, Tatiana V. Chepurnykh, Nadezhda M. Markina, Sergey I. Kovalchuk, Aleksandra S. Tsarkova, Alexander S. Mishin, Ilia V. Yampolsky, Karen S. Sarkisyan
Publikováno v:
International Journal of Molecular Sciences, Vol 24, Iss 2, p 1317 (2023)
Hispidin is a polyketide found in plants and fungi. In bioluminescent fungi, hispidin serves as a precursor of luciferin and is produced by hispidin synthases. Previous studies revealed that hispidin synthases differ in orthologous polyketide synthas
Externí odkaz:
https://doaj.org/article/f93dc3b87caa486690f4170d73bdb166