Zobrazeno 1 - 10
of 15
pro vyhledávání: '"Alexander N. Asanov"'
Autor:
Mark J. Jedrzejas, Alexander N. Asanov, W. William Wilson, Philip B. Oldham, Heather M. McDonald
Publikováno v:
Journal of Crystal Growth. 232:603-609
Genomics is on the verge of creating a large number of proteins for structure determination. Since X-ray crystallography is the main tool for determining protein structure, a corresponding large number of X-ray diffraction experiments will have to be
Publikováno v:
Microchemical Journal. 70:63-68
Total internal reflection fluorescence (TIRF) is widely used for investigating interfacial interactions. It has proven to be a well-suited technique for biosensing applications due to its surface sensitivity and minimal sample consumption. In this pa
Publikováno v:
Journal of Crystal Growth. 196:424-433
The osmotic second virial coefficient, B , is a dilute solution parameter, so one may wonder what relationships exist among B and the many facets of protein crystal growth (PCG). In particular, knowledge of how a protein's solubility ( S ) depends on
Publikováno v:
Instrumentation Science & Technology. 26:375-387
In conventional fluorescence spectroscopy, fluorescence intensity at high fluorophore concentration is often not proportional to fluorophore concentration, owing to primary and secondary absorption (inner-filter effects). In this paper, fluorescence
Publikováno v:
Journal of Colloid and Interface Science. 196:62-73
Total internal reflection fluorescence (TIRF) was used to examine adsorption and interfacial aggregation of bovine serum albumin (BSA) from solvent conditions that are known to promote BSA crystallization. In the absence of a crystallizing agent (the
Publikováno v:
Encyclopedia of Analytical Chemistry
The physical and chemical characteristics of surfaces along with the structure and dynamics of molecules near surfaces or interfaces have recently become critically important. Surface measurements have provided important information concerning the pr
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::09e4200bc404f6ecd8ed50b5ccdd9fab
https://doi.org/10.1002/9780470027318.a5414
https://doi.org/10.1002/9780470027318.a5414
Publikováno v:
SPIE Proceedings.
A total internal reflection fluorescence (TIRF) flow system (BioElectroSpec, Inc.) was used to detect DNA adsorption and hybridization of a single stranded target DNA with a surface immobilized DNA probe. A transparent SnO 2 or indium tin oxide (ITO)
Autor:
Philip B. Oldham, Alexander N. Asanov
Publikováno v:
SPIE Proceedings.
The development of a biosensor with adequate sensitivity generally requires a biospecific interaction with high binding affinity. The affinity constants for most antibody-antigen interactions are determined largely by the dissociation constants, k d
Publikováno v:
Analytical chemistry. 70(6)
A new biosensor platform that provides simultaneous fluorescence detection and electrochemical control of biospecific binding has been developed and investigated using antibody-antigen and streptavidin-biotin interactions. Specifically, biotin was co
Publikováno v:
Journal of colloid and interface science. 191(1)
Total internal reflection fluorescence (TIRF) was applied to study adsorption of bovine serum albumin (BSA) from a thermodynamically good solvent commonly used as a buffer for BSA crystallization. BSA adsorption is shown to exhibit heteroenergetics,