Multiple amino acid sequence alignment nitrogenase component 1: insights into phylogenetics and structure-function relationships.

Autor: James B Howard, Katerina J Kechris, Douglas C Rees, Alexander N Glazer

Publikováno v: PLoS ONE, Vol 8, Iss 9, p e72751 (2013)

Amino acid residues critical for a protein's structure-function are retained by natural selection and these residues are identified by the level of variance in co-aligned homologous protein sequences. The relevant residues in the nitrogen fixation Co

Externí odkaz: https://doaj.org/article/3603777c621042f3a66a83315c82155f

The Evolution and Future of Earth’s Nitrogen Cycle

Autor: Alexander N. Glazer, Paul G. Falkowski, Donald E. Canfield

Publikováno v: Canfield, D E, Glazer, A N & Falkowski, P G 2010, ' The Evolution and Future of Earth's Nitrogen Cycle ', Science, vol. 330, no. 6001, pp. 192-196 . https://doi.org/10.1126/science.1186120

Atmospheric reactions and slow geological processes controlled Earth's earliest nitrogen cycle, and by similar to 2.7 billion years ago, a linked suite of microbial processes evolved to form the modern nitrogen cycle with robust natural feedbacks and

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::dc4448f4d04cd53e085a233dcf8774a0
https://doi.org/10.1126/science.1186120

Finding important sites in protein sequences

Autor: Peter J. Bickel, Katherina J. Kechris, Gary J. Wedemayer, Alexander N. Glazer, Philip C. Spector

Publikováno v: Proceedings of the National Academy of Sciences. 99:14764-14771

By using sequence information from an aligned protein family, a procedure is exhibited for finding sites that may be functionally or structurally critical to the protein. Features based on sequence conservation within subfamilies in the alignment and

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::5e36f46de700a7204ee42aeae6479b4e
https://doi.org/10.1073/pnas.222508899

Biosynthesis of a fluorescent cyanobacterial C-phycocyanin holo-α subunit in a heterologous host

Autor: Yuping A. Cai, Alexander N. Glazer, Aaron J. Tooley

Publikováno v: Proceedings of the National Academy of Sciences. 98:10560-10565

The entire pathway for the synthesis of a fluorescent holophycobiliprotein subunit from a photosynthetic cyanobacterium ( Synechocystis sp. PCC6803) was reconstituted in Escherichia coli . Cyanobacterial genes encoding enzymes required for the conver

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::5fd31c383c775fd217942a66df4dfcba
https://doi.org/10.1073/pnas.181340998

Recombinant Phycobiliproteins

Autor: Alexander N. Glazer, Yuping A. Cai, John T. Murphy, Gary J. Wedemayer

Publikováno v: Analytical Biochemistry. 290:186-204

A family of specific cloning vectors was constructed to express in the cyanobacterium Anabaena sp. PCC7120 recombinant C-phycocyanin subunits with one or more different tags, including the 6xHis tag, oligomerization domains, and the streptavidin-bind

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_________::cfda68d38184e5e2527e607fc9f769a5
https://doi.org/10.1006/abio.2000.4979