Bioorthogonal labeling of transmembrane proteins with non-canonical amino acids unveils masked epitopes in live neurons

Autor: Diogo Bessa-Neto, Gerti Beliu, Alexander Kuhlemann, Valeria Pecoraro, Sören Doose, Natacha Retailleau, Nicolas Chevrier, David Perrais, Markus Sauer, Daniel Choquet

Publikováno v: Nature Communications, Vol 12, Iss 1, Pp 1-16 (2021)

Visualisation of TARP localisation is hindered by existing imaging tools. Here the authors report a labelling and imaging platform using genetic code expansion and non-canonical amino acids; they use this to fluorescently label live neurons and local

Externí odkaz: https://doaj.org/article/1dfd358afde74c72bc8f54e8fcd08c90

The Neutral Sphingomyelinase 2 Is Required to Polarize and Sustain T Cell Receptor Signaling

Autor: Charlene Börtlein, Annette Draeger, Roman Schoenauer, Alexander Kuhlemann, Markus Sauer, Sibylle Schneider-Schaulies, Elita Avota

Publikováno v: Frontiers in Immunology, Vol 9 (2018)

By promoting ceramide release at the cytosolic membrane leaflet, the neutral sphingomyelinase 2 (NSM) is capable of organizing receptor and signalosome segregation. Its role in T cell receptor (TCR) signaling remained so far unknown. We now show that

Externí odkaz: https://doaj.org/article/6c049a1c658e433bbd1cfdc2bd0d7a32

Improved biocatalytic cascade conversion of CO2 to methanol by enzymes Co-immobilized in tailored siliceous mesostructured cellular foams

Autor: Anders Palmqvist, Milene Zezzi do Valle Gomes, Markus Sauer, Gerard Masdeu, Alexander Kuhlemann, Patrick Eiring, Björn Åkerman

Publikováno v: Catalysis Science & Technology. 11:6952-6959

CO2 can be enzymatically reduced to methanol in a cascade reaction involving three enzymes: formate-, formaldehyde- and alcohol dehydrogenase (FateDH, FaldDH, ADH). We report an improvement in the yield of this reaction by co-immobilizing the three d

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_________::daf9314c2a4cae85cdb1096404337c00
https://doi.org/10.1039/d1cy01354h

Bioorthogonal labeling of transmembrane proteins with non-canonical amino acids unveils masked epitopes in live neurons

Autor: Gerti Beliu, David Perrais, Alexander Kuhlemann, Valeria Pecoraro, Sören Doose, Daniel Choquet, Natacha Retailleau, Nicolas Chevrier, Diogo Bessa-Neto, Markus Sauer

Publikováno v: Nature Communications
Nature Communications, Nature Publishing Group, 2021, 12 (1), ⟨10.1038/s41467-021-27025-w⟩
Nature Communications, Vol 12, Iss 1, Pp 1-16 (2021)

Progress in biological imaging is intrinsically linked to advances in labeling methods. The explosion in the development of high-resolution and super-resolution imaging calls for new approaches to label targets with small probes. These should allow t

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::6539e2a72b61cb5cb6f41daf0402a196
https://doi.org/10.1038/s41467-021-27025-w

Bioorthogonal labeling with tetrazine-dyes for super-resolution microscopy

Autor: Martin J. Schnermann, Markus Sauer, Sören Doose, Jonathan B. Grimm, Zhen-Dan Shi, Mara Meub, Luke D. Lavis, Andreas Kurz, Lisa Behringer-Pliess, Natalia Wolf, Gerti Beliu, Alexander Kuhlemann, Jürgen Seibel

Publikováno v: Communications Biology
Communications Biology, Vol 2, Iss 1, Pp 1-13 (2019)

Genetic code expansion (GCE) technology allows the specific incorporation of functionalized noncanonical amino acids (ncAAs) into proteins. Here, we investigated the Diels-Alder reaction between trans-cyclooct-2-ene (TCO)-modified ncAAs, and 22 known

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::8988d69f5133c64e2106fed38788a97a
https://pubmed.ncbi.nlm.nih.gov/31341960