Zobrazeno 1 - 8
of 8
pro vyhledávání: '"Alexander Hillar"'
Publikováno v:
Glycobiology. 22:417-428
The inability to produce recombinant glycoproteins with authentic N-glycans is a limitation of many heterologous protein expression systems. In the baculovirus–insect cell system, this limitation has been addressed by glycoengineering insect cell l
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::1e4ba35b4eb3cb619931920441e95302
https://doi.org/10.1093/glycob/cwr160
https://doi.org/10.1093/glycob/cwr160
Publikováno v:
Protein Expression and Purification. 55:246-256
To generate hemoglobin-free full-length haptoglobin the cDNA encoding rat haptoglobin alphabeta subunits was cloned into shuttle vector pVT-Bac-His and used to produce a recombinant baculovirus Autographa californica Nuclear Polyhedrosis Virus (AcNPV
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::3bf7409d6f8fc7beefbee30f6ff85a49
https://doi.org/10.1016/j.pep.2007.06.004
https://doi.org/10.1016/j.pep.2007.06.004
Autor:
Feng Liu, Doreen E. Culham, Robert A. B. Keates, Joan M. Boggs, Yaroslava I. Vernikovska, Janet M. Wood, Alexander Hillar
Publikováno v:
Biochemistry. 44:5634-5646
Transporter ProP of Escherichia coli, a member of the major facilitator superfamily (MFS), acts as an osmosensor and an osmoregulator in cells and after purification and reconstitution in proteoliposomes. H(+)-osmoprotectant symport via ProP is activ
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::d6d4c67bd6b5e4062bbca9046c61b22e
https://doi.org/10.1021/bi047383o
https://doi.org/10.1021/bi047383o
Autor:
Robert S. Hodges, Brian Tripet, David Zoetewey, Joan M. Boggs, Alexander Hillar, Janet M. Wood
Publikováno v:
Biochemistry. 42:15170-15178
Electron paramagnetic resonance spectroscopy was used to determine relative peptide orientation within homodimeric, alpha-helical coiled-coil structures. Introduction of cysteine (Cys) residues into peptides/proteins for spin labeling allows detectio
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::bd643b044cf740db046ef7b01a940f4d
https://doi.org/10.1021/bi035122t
https://doi.org/10.1021/bi035122t
Autor:
James Henderson, Anh Ly, Alexander Hillar, Kathleen I. Racher, Joan M. Boggs, Yaroslava I. Vernikovska, Janet M. Wood, Doreen E. Culham
Publikováno v:
Biochemistry. 42:11815-11823
Transporter ProP of Escherichia coli is an osmosensor and an osmoprotectant transporter. Previous results suggest that medium osmolality determines the proportions of ProP in active and inactive conformations. A cysteine-less (Cys-less) variant was c
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::af1c148c2fe7daf4bef862616d3c2a09
https://doi.org/10.1021/bi034939j
https://doi.org/10.1021/bi034939j
Publikováno v:
Universe, Vol 9, Iss 10, p 442 (2023)
Radio bursts provide important diagnostics of energetic phenomena of the Sun. In particular, bursts in decimetric and metric wavelengths probe the physical conditions and the energy release processes in the low corona as well as their association wit
Externí odkaz:
https://doaj.org/article/ee95fdcc5cb3405299b9588eef83696a
Autor:
Alexander Hillar, Donald L. Jarvis
Publikováno v:
Glycobiology. 20(10)
It was previously reported that Tn-4h and DpN1 cells have the endogenous capacity to efficiently sialylate secreted alkaline phosphatase (SEAP) when infected with a baculovirus expression vector. In contrast, it has been found that lepidopteran insec
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::200e267407baa7bcf82d59bb12d23243
https://pubmed.ncbi.nlm.nih.gov/20574041
https://pubmed.ncbi.nlm.nih.gov/20574041
Publikováno v:
Biochemistry. 44:15636-15636
Membrane transporter ProP from Escherichia coli senses extracellular osmolality and responds by mediating the uptake of osmoprotectants such as glycine betaine when osmolality is high. Earlier EPR and NMR studies showed that a peptide replica of the
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::8e4bebe361b785708b691f33fb8300c6
https://doi.org/10.1021/bi058033k
https://doi.org/10.1021/bi058033k