Zobrazeno 1 - 10
of 82
pro vyhledávání: '"Alexander A. Kortt"'
Autor:
Alexander A. Kortt, L.C. Gruen
Publikováno v:
International Journal of Peptide and Protein Research. 25:389-397
Spectrophotometric measurement was found to be a sensitive method for evaluating the stability of the chymotrypsin inhibitor from the winged bean. The thermal stability of this protein in aqueous solution was much greater at pH 3 than at pH 8 or pH 1
Autor:
Alexander A. Kortt
Publikováno v:
International Journal of Peptide and Protein Research. 28:613-619
The albumin fraction of the seeds of winged bean Psophocarpus tetragonolobus (L.) DC contains the antinutritional factors and several other proteins that are significant components of the total seed protein (˜ 30%). The major protein in the albumin
Autor:
Michael A. Jermyn, Alexander A. Kortt
Publikováno v:
European Journal of Biochemistry. 115:551-558
Two trypsin inhibitors from the seed of Acacia elata, a legume of the subfamily Mimosoideae, were isolated by affinity chromatography on trypsin-Sepharose 4B and separated by chromatography on SP-Sephadex C-25 and Sephadex G-100. The two inhibitors,
Autor:
Alexander A. Kortt, Dean R. Hewish, Pamela J. Russell, Katy Sterling-Levis, Teresa Carter, Meghan Hattarki, Kim Ow, Deborah Shapira, Larissa Doughty
Publikováno v:
Cancer Immunology, Immunotherapy. 53:533-542
Background: Monoclonal antibodies (MAbs) are used for targeting agents to tumours while minimizing normal tissue exposure. Methods: A new anti–prostate cancer MAb, BLCA-38, was radioiodinated (I125) and assessed for its ability to target subcutaneo
Autor:
Alexander A. Kortt, Peter J. Hudson, Mark Walter, Olan Dolezal, Meghan Hattarki, Larissa Doughty, Ross De Gori
Publikováno v:
Protein Engineering, Design and Selection. 16:47-56
Single-chain variable fragment of the murine monoclonal antibody NC10 specific to influenza virus N9 neuraminidase, joined directly in the V(L) to V(H) orientation (scFv-0), forms an equilibrium mixture of tetramer and trimer with the tetramer as the
Publikováno v:
Journal of Immunological Methods. 262:217-227
This protocol describes optimised large-scale bacterial fermentation conditions for recombinant single-chain Fv molecule (scFv) monomers and multimers (diabodies and triabodies). The heat-inducible bacterial secretion vector, pPOW3, utilising the tem
Autor:
Peter J. Hudson, Larissa Doughty, Anne Nathanielsz, Robert N. Pike, Alexander A. Kortt, Nafisa Ally, Usha V. Krishnan, Robert Alexander Irving, Stewart D. Nuttall
Publikováno v:
FEBS Letters. 516:80-86
The new antigen receptor (NAR) from sharks consists of a single immunoglobulin variable domain attached to five constant domains, and is hypothesised to function as an antibody. Two closely related NARs with affinity for the Kgp (lysine-specific) gin
Publikováno v:
Biomolecular Engineering. 18:95-108
Recombinant antibody fragments can be engineered to assemble into stable multimeric oligomers of high binding avidity and specificity to a wide range of target antigens and haptens. This review describes the design and expression of diabodies (dimers
Autor:
Barbara E. Power, Fiona E Smyth, Alexander A. Kortt, Andrew M. Scott, John E. Burns, Joanne M. Caine, Deborah Shapira, Meghan Hattarki, Peter J. Hudson, Kiki Tahtis, Fook-Thean Lee
Publikováno v:
Cancer Immunology, Immunotherapy. 50:241-250
A single-chain antibody fragment (scFv) of the humanised monoclonal antibody, hu3S193, that reacts specifically with Le(y) antigen expressed in numerous human epithelial carcinomas was constructed. A five-residue linker joined the C-terminus of the V
Autor:
Alexander A. Kortt, Peter J. Hudson
Publikováno v:
Journal of Immunological Methods. 231:177-189
Multivalent recombinant antibody fragments provide high binding avidity and unique specificity to a wide range of target antigens and haptens. This review describes how careful choice of linker length between V-domains creates new types of Fv modules