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pro vyhledávání: '"Alex von Diezmann"'
Automated processing of double-helix (DH) microscope images of fluorescent single molecules (SMs) streamlines the protocol required to obtain super-resolved three-dimensional (3D) reconstructions of ultrastructures in biological samples by single-mol
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::313feb83f87976b3656d67393414a8d1
https://doi.org/10.21203/rs.2.9151/v1
https://doi.org/10.21203/rs.2.9151/v1
Autor:
Alex von Diezmann, Thomas H. Mann, Keren Lasker, Daniel G. Ahrens, Lucy Shapiro, W. E. Moerner
SUMMARYSelective recruitment and concentration of signaling proteins within membrane-less compartments is a ubiquitous mechanism for subcellular organization. However, little is known about how such dynamic recruitment patterns intracellular signalin
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::a201f722379e4e8f296ec88eea2fd6f7
Publikováno v:
Chemical reviews. 117(11)
Single-molecule super-resolution fluorescence microscopy and single-particle tracking are two imaging modalities that illuminate the properties of cells and materials on spatial scales down to tens of nanometers, or with dynamical information about n
Publikováno v:
Biophysical Journal. 116:453a
3D Single-Molecule Super-Resolution Fluorescence Microscopy with the Corkscrew Point Spread Function
Autor:
W. E. Moerner, Yoav Shechtman, Matthew D. Lew, Maurice Lee, Lucien E. Weiss, Alex von Diezmann
Publikováno v:
Biophysical Journal. 110(3)
The Moerner Laboratory has been engineering the point spread function (PSF) of a wide-field fluorescence microscope in order to obtain simultaneous X, Y, and Z spatial information from single molecules over a large Z-range. The new PSFs (e.g., the do
Autor:
Thomas H. Mann, Alex von Diezmann, Daniel G. Ahrens, Keren Lasker, Lucy Shapiro, W. E. Moerner
Publikováno v:
Biophysical Journal. 114:548a
The localization of single fluorescent molecules enables the imaging of molecular structure and dynamics with subdiffraction precision and can be extended to three dimensions using point spread function (PSF) engineering. However, the nanoscale accur
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::f90150d257ab2e3aaa0ee9c1f011de51
https://europepmc.org/articles/PMC4782984/
https://europepmc.org/articles/PMC4782984/