Zobrazeno 1 - 7
of 7
pro vyhledávání: '"Alex S. Shcheglov"'
Autor:
Alex S. Shcheglov, Veronika E. Anisimova, Ekaterina A. Bogdanova, Alexey N. Nekrasov, Dmitry A. Shagin, Denis V. Rebrikov, Ekaterina V. Barsova, Sergey Lukyanov
Publikováno v:
Gene. 418:41-48
Kamchatka crab duplex-specific nuclease (Par_DSN) has been classified as a member of the family of DNA/RNA non-specific beta-beta-alpha metal finger (bba-Me-finger) nucleases, the archetype of which is the nuclease from Serratia marcescens. Although
Autor:
P A Zhulidov, Ekaterina A. Bogdanova, Dmitry A. Shagin, G L Khazpekov, Luk'ianov Sa, V V Kozhemiako, Irina A. Shagina, Vagner Ll, Alex S. Shcheglov
Publikováno v:
Russian Journal of Bioorganic Chemistry. 31:170-177
We developed a new method for the preparation of normalized cDNA libraries enriched with full-length sequences. It is based on the properties of the recently characterized duplex-specific nuclease from the hepatopancreas of the Kamchatka crab. The du
Autor:
Dmitry A. Shagin, Valery B. Kozhemyako, Ekaterina A. Bogdanova, Alex S. Shcheglov, Ilia M. Altshuler, Sergey Lukyanov, Denis V. Rebrikov, Pavel A. Zhulidov, Dmitry B. Staroverov, Valery A. Rasskazov
Publikováno v:
Genome Research. 12:1935-1942
We have characterized a novel nuclease from the Kamchatka crab, designated duplex-specific nuclease (DSN). DSN displays a strong preference for cleaving double-stranded DNA and DNA in DNA-RNA hybrid duplexes, compared to single-stranded DNA. Moreover
Autor:
Veronika E. Anisimova, Alex S. Shcheglov, Ekaterina A. Bogdanova, Sergey Lukyanov, Ekaterina V. Barsova
Publikováno v:
Biotechnology letters. 31(2)
Using random mutagenesis of the gene encoding duplex-specific nuclease from the king crab we found a new mutant that retained all properties of the wild-type protein, but exhibited a much lower thermal stability. This enzyme, denoted thermolabile dup
Publikováno v:
Nucleic Acids Hybridization Modern Applications ISBN: 9781402060397
In a cellular transcriptome, the number of mRNA copies per gene may differ by several orders. In cDNA libraries, performed from mRNA, these proportions are the same. Normalization methods allow us to equalize numbers of gene’s copies in the library
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::c002b45eb25718dbce39db9be32d4341
https://doi.org/10.1007/978-1-4020-6040-3_5
https://doi.org/10.1007/978-1-4020-6040-3_5
Autor:
Alex S. Shcheglov, Ekaterina A. Bogdanova, Valery B. Kozhemyako, Sergey Lukyanov, George L. Khaspekov, Leonid L. Moroz, Ella A. Meleshkevitch, Mikhail V. Matz, Vagner Ll, Dmitry A. Shagin, Pavel A. Zhulidov
Publikováno v:
Nucleic Acids Research. 32:37e-37
We developed a novel simple cDNA normalization method [termed duplex-specific nuclease (DSN) normalization] that may be effectively used for samples enriched with full-length cDNA sequences. DSN normalization involves the denaturation-reassociation o
Autor:
Sergey Lukyanov, Dmitry A. Shagin, Dmitry B. Staroverov, Valery A. Rasskazov, Valery B. Kozhemyako, Vagner Ll, Veronika E. Anisimova, Rustam Ziganshin, Natalia I Menzorova, Alex S. Shcheglov, Denis V. Rebrikov
Publikováno v:
BMC Biochemistry, Vol 9, Iss 1, p 14 (2008)
BMC Biochemistry
BMC Biochemistry
Background Nucleases, which are key components of biologically diverse processes such as DNA replication, repair and recombination, antiviral defense, apoptosis and digestion, have revolutionized the field of molecular biology. Indeed many standard m