Zobrazeno 1 - 10
of 68
pro vyhledávání: '"Alex J. Noble"'
Autor:
Anchi Cheng, Paul T. Kim, Huihui Kuang, Joshua H. Mendez, Eugene Y. D. Chua, Kashyap Maruthi, Hui Wei, Anjelique Sawh, Mahira F. Aragon, Viacheslav Serbynovskyi, Kasahun Neselu, Edward T. Eng, Clinton S. Potter, Bridget Carragher, Tristan Bepler, Alex J. Noble
Publikováno v:
IUCrJ, Vol 10, Iss 1, Pp 77-89 (2023)
Single-particle cryo-electron microscopy (cryoEM) is a swiftly growing method for understanding protein structure. With increasing demand for high-throughput, high-resolution cryoEM services comes greater demand for rapid and automated cryoEM grid an
Externí odkaz:
https://doaj.org/article/38ddd6438e3345ae9cf649c9abb5975b
Publikováno v:
IUCrJ, Vol 10, Iss 1, Pp 90-102 (2023)
Over the past decade, cryo-electron microscopy (cryoEM) has emerged as an important method for determining near-native, near-atomic resolution 3D structures of biological macromolecules. To meet the increasing demand for cryoEM, automated methods tha
Externí odkaz:
https://doaj.org/article/ec1a47dff0404b9a8981ff2f6acd35cc
Autor:
Kotaro Kelley, Ashleigh M. Raczkowski, Oleg Klykov, Pattana Jaroenlak, Daija Bobe, Mykhailo Kopylov, Edward T. Eng, Gira Bhabha, Clinton S. Potter, Bridget Carragher, Alex J. Noble
Publikováno v:
Nature Communications, Vol 13, Iss 1, Pp 1-13 (2022)
Here the authors describe the Waffle Method, aimed at increasing the throughput of and solves several challenges present in cryo-FIB/SEM sample preparation for cryo-ET analysis — the highest-resolution method for obtaining 3D views of native biolog
Externí odkaz:
https://doaj.org/article/e079e893c227458c8f9d1347d6358ed8
Autor:
Wenguang G. Liang, Juwina Wijaya, Hui Wei, Alex J. Noble, Jordan M. Mancl, Swansea Mo, David Lee, John V. Lin King, Man Pan, Chang Liu, Carla M. Koehler, Minglei Zhao, Clinton S. Potter, Bridget Carragher, Sheng Li, Wei-Jen Tang
Publikováno v:
Nature Communications, Vol 13, Iss 1, Pp 1-14 (2022)
Presequence protease (PreP) is essential to mitochondrial proteostasis. This study leverages advanced vitrification techniques to solve cryoEM structures of apo- and substrate-bound PreP and integrates these data with other analysis to reveal key sta
Externí odkaz:
https://doaj.org/article/d99e3402e9504dfda0d7d987246a3e86
Autor:
Natalia G. Herrera, Nicholas C. Morano, Alev Celikgil, George I. Georgiev, Ryan J. Malonis, James H. Lee, Karen Tong, Olivia Vergnolle, Aldo B. Massimi, Laura Y. Yen, Alex J. Noble, Mykhailo Kopylov, Jeffrey B. Bonanno, Sarah C. Garrett-Thomson, David B. Hayes, Robert H. Bortz, Ariel S. Wirchnianski, Catalina Florez, Ethan Laudermilch, Denise Haslwanter, J. Maximilian Fels, M. Eugenia Dieterle, Rohit K. Jangra, Jason Barnhill, Amanda Mengotto, Duncan Kimmel, Johanna P. Daily, Liise-anne Pirofski, Kartik Chandran, Michael Brenowitz, Scott J. Garforth, Edward T. Eng, Jonathan R. Lai, Steven C. Almo
Publikováno v:
ACS Omega, Vol 6, Iss 1, Pp 85-102 (2020)
Externí odkaz:
https://doaj.org/article/8225fcf771154e9c93f8122d956b1bc4
Publikováno v:
Nature Communications, Vol 11, Iss 1, Pp 1-12 (2020)
The low signal-to-noise ratio (SNR) in cryoEM images can make the first steps in cryoEM structure determination challenging, particularly for non-globular and small proteins. Here, the authors present Topaz-Denoise, a deep learning based method for m
Externí odkaz:
https://doaj.org/article/e50a3027e68f4f018f4520844d5b05ae
Autor:
Oliver J. Harrison, Julia Brasch, Phinikoula S. Katsamba, Goran Ahlsen, Alex J. Noble, Hanbin Dan, Rosemary V. Sampogna, Clinton S. Potter, Bridget Carragher, Barry Honig, Lawrence Shapiro
Publikováno v:
Cell Reports, Vol 30, Iss 8, Pp 2655-2671.e7 (2020)
Summary: Non-clustered δ1- and δ2-protocadherins, close relatives of clustered protocadherins, function in cell adhesion and motility and play essential roles in neural patterning. To understand the molecular interactions underlying these functions
Externí odkaz:
https://doaj.org/article/8f90b79156da49fcad93fd395bf1ec18
Autor:
Qing Xie, John M. Spear, Alex J. Noble, Duncan R. Sousa, Nancy L. Meyer, Omar Davulcu, Fuming Zhang, Robert J. Linhardt, Scott M. Stagg, Michael S. Chapman
Publikováno v:
Molecular Therapy: Methods & Clinical Development, Vol 5, Iss C, Pp 1-12 (2017)
Atomic structures of adeno-associated virus (AAV)-DJ, alone and in complex with fondaparinux, have been determined by cryoelectron microscopy at 3 Å resolution. The gene therapy vector, AAV-DJ, is a hybrid of natural serotypes that was previously de
Externí odkaz:
https://doaj.org/article/fb211d03182c45d4b321803376200cb3
Publikováno v:
Journal of Structural Biology: X, Vol 1, Iss , Pp - (2019)
Preferred particle orientation presents a major challenge for many single particle cryo-electron microscopy (cryo-EM) samples. Orientation bias limits the angular information used to generate three-dimensional maps and thus affects the reliability an
Externí odkaz:
https://doaj.org/article/046343c6e46c424597ee19441e89be65
Nuclear processes depend on the organization of chromatin, whose basic units are cylinder-shaped complexes called nucleosomes. A subset of mammalian nucleosomes in situ (inside cells) resembles the canonical structure determined in vitro 25 years ago
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::2e0600a20428e0f14f304bc48fdb98a1
https://doi.org/10.7554/elife.87672.1
https://doi.org/10.7554/elife.87672.1
