Zobrazeno 1 - 10
of 97
pro vyhledávání: '"Alex J Walsh"'
Autor:
Oscar R Benavides, Holly C Gibbs, Berkley P White, Roland Kaunas, Carl A Gregory, Alex J Walsh, Kristen C Maitland
Publikováno v:
PLoS ONE, Vol 18, Iss 3, p e0282298 (2023)
The adoption of cell-based therapies into the clinic will require tremendous large-scale expansion to satisfy future demand, and bioreactor-microcarrier cultures are best suited to meet this challenge. The use of spherical microcarriers, however, pre
Externí odkaz:
https://doaj.org/article/a5178f117ef6445c878efd23b6b95c1f
Publikováno v:
PLoS ONE, Vol 18, Iss 3, p e0283692 (2023)
Many techniques and software packages have been developed to segment individual cells within microscopy images, necessitating a robust method to evaluate images segmented into a large number of unique objects. Currently, segmented images are often co
Externí odkaz:
https://doaj.org/article/11141f744cab4c9081303b26d23b5daa
Autor:
Veronika Miskolci, Kelsey E Tweed, Michael R Lasarev, Emily C Britt, Alex J Walsh, Landon J Zimmerman, Courtney E McDougal, Mark R Cronan, Jing Fan, John-Demian Sauer, Melissa C Skala, Anna Huttenlocher
Publikováno v:
eLife, Vol 11 (2022)
The function of macrophages in vitro is linked to their metabolic rewiring. However, macrophage metabolism remains poorly characterized in situ. Here, we used two-photon intensity and lifetime imaging of autofluorescent metabolic coenzymes, nicotinam
Externí odkaz:
https://doaj.org/article/8206c968188744b0808bd43cb3275c27
Autor:
Woojong Lee, Brock Kingstad-Bakke, Brett Paulson, Autumn Larsen, Katherine Overmyer, Chandranaik B Marinaik, Kelly Dulli, Randall Toy, Gabriela Vogel, Katherine P Mueller, Kelsey Tweed, Alex J Walsh, Jason Russell, Krishanu Saha, Leticia Reyes, Melissa C Skala, John-Demian Sauer, Dmitry M Shayakhmetov, Joshua Coon, Krishnendu Roy, M Suresh
Publikováno v:
PLoS Pathogens, Vol 17, Iss 1, p e1009168 (2021)
There is a critical need for adjuvants that can safely elicit potent and durable T cell-based immunity to intracellular pathogens. Here, we report that parenteral vaccination with a carbomer-based adjuvant, Adjuplex (ADJ), stimulated robust CD8 T-cel
Externí odkaz:
https://doaj.org/article/73580a7e1e6e47af817c8a1751850e37
Autor:
Amy T Shah, Michelle Demory Beckler, Alex J Walsh, William P Jones, Paula R Pohlmann, Melissa C Skala
Publikováno v:
PLoS ONE, Vol 9, Iss 3, p e90746 (2014)
Optical metabolic imaging measures fluorescence intensity and lifetimes from metabolic cofactors nicotinamide adenine dinucleotide (NADH) and flavin adenine dinucleotide (FAD). These molecular level measurements provide unique biomarkers for early ce
Externí odkaz:
https://doaj.org/article/98c9dbfc2d3b4e6d9b2e8bcd605ce861
Autor:
Wangie Yu, Yunyun Chen, Nagireddy Putluri, Abdullah Osman, Cristian Coarfa, Vasanta Putluri, Abu H. M. Kamal, Jennifer Kay Asmussen, Panagiotis Katsonis, Jeffrey N. Myers, Stephen Y. Lai, Wuhao Lu, Clifford C. Stephan, Reid T. Powell, Faye M. Johnson, Heath D. Skinner, Jawad Kazi, Kazi Mokim Ahmed, Linghao Hu, Addison Threet, Matthew D. Meyer, James A. Bankson, Tony Wang, Jack Davis, Kirby R. Parker, Madison A. Harris, Mokryun L. Baek, Gloria V. Echeverria, Xiaoli Qi, Jin Wang, Andy I. Frederick, Alex J. Walsh, Olivier Lichtarge, Mitchell J. Frederick, Vlad C. Sandulache
Publikováno v:
British Journal of Cancer. 128:2013-2024
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::154d3749722bfad6e9f8084a8ee3b730
https://doi.org/10.1038/s41416-023-02253-7
https://doi.org/10.1038/s41416-023-02253-7
Autor:
Melissa C. Skala, Carlos L. Arteaga, Gennaro Ciliberto, Luigi Aurisicchio, Melinda E. Sanders, Rebecca S. Cook, Alex J. Walsh
Supplementary Figure 1: OMI endpoints of organoids derived from a trastuzumabresponsive xenograft tumor. Supplementary Figure 2: Population modeling identifies heterogeneous drug responses of BT474 cells. Supplementary Figure 3: OMI endpoints of orga
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::3422702523ec1f8f647f81f66fb67cdd
https://doi.org/10.1158/0008-5472.22402950
https://doi.org/10.1158/0008-5472.22402950
Autor:
Melissa C. Skala, Carlos L. Arteaga, Alec Lafontant, Donna J. Hicks, H. Charles Manning, Rebecca S. Cook, Alex J. Walsh
PDF file - 198K, Breast cancer cell lines and receptor expression (S1); Correlations among optical metabolic measurements (S2).
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::6d28251572884c64dfac2ea2bc0a1ec6
https://doi.org/10.1158/0008-5472.22397814
https://doi.org/10.1158/0008-5472.22397814
Autor:
Melissa C. Skala, Carlos L. Arteaga, Alec Lafontant, Donna J. Hicks, H. Charles Manning, Rebecca S. Cook, Alex J. Walsh
Abnormal cellular metabolism is a hallmark of cancer, yet there is an absence of quantitative methods to dynamically image this powerful cellular function. Optical metabolic imaging (OMI) is a noninvasive, high-resolution, quantitative tool for monit
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::0f311bd5e0bd78540b205c5de9aa7497
https://doi.org/10.1158/0008-5472.c.6504921.v1
https://doi.org/10.1158/0008-5472.c.6504921.v1
Autor:
Melissa C. Skala, Carlos L. Arteaga, Alec Lafontant, Donna J. Hicks, H. Charles Manning, Rebecca S. Cook, Alex J. Walsh
PDF file - 783K, Cyanide addition to MCF10A cells changes NADH and FAD fluorescence lifetime components (S1); The optical redox ratio increases with ER and HER2 expression (S2); NADH and FAD fluorescence lifetime components for breast cancer cells (S
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::9d6abfbed740e9255b1b16ce110714ba
https://doi.org/10.1158/0008-5472.22397817.v1
https://doi.org/10.1158/0008-5472.22397817.v1