A fusion of the Bacteroides fragilis ferrous iron import proteins reveals a role for FeoA in stabilizing GTP-bound FeoB

Autor: Alex E. Sestok, Janae B. Brown, Juliet O. Obi, Sean M. O’Sullivan, Elsa D. Garcin, Daniel J. Deredge, Aaron T. Smith

Publikováno v: Journal of Biological Chemistry
Journal of Biological Chemistry, 2022, 298 (4), pp.101808. ⟨10.1016/j.jbc.2022.101808⟩

International audience; Iron is an essential element for nearly all organisms, and under anoxic and/or reducing conditions, Fe2+ is the dominant form of iron available to bacteria. The ferrous iron transport (Feo) system is the primary prokaryotic Fe

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::f27c210d3dac2aff2f9e074b8b8c94be
https://pubmed.ncbi.nlm.nih.gov/35271852

A general protocol for the expression and purification of the intact transmembrane transporter FeoB

Autor: Alex E, Sestok, Sean M, O'Sullivan, Aaron T, Smith

Publikováno v: Biochim Biophys Acta Biomembr

Ferrous iron (Fe(2+)) transport is an essential process that supports the growth, intracellular survival, and virulence of several drug-resistant pathogens, and the ferrous iron transport (Feo) system is the most important and widespread protein comp

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::6359bd21a256c98374afe2364c3b5c42
https://doi.org/10.1016/j.bbamem.2022.183973