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Akademický článek

Non-viral DNA delivery and TALEN editing correct the sickle cell mutation in hematopoietic stem cells

Publikováno v: Nature Communications, Vol 15, Iss 1, Pp 1-21 (2024)

Abstract Sickle cell disease is a devastating blood disorder that originates from a single point mutation in the HBB gene coding for hemoglobin. Here, we develop a GMP-compatible TALEN-mediated gene editing process enabling efficient HBB correction v

Externí odkaz: https://doaj.org/article/a5ed07ba92514c729d823ca1c6aa5120

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Akademický článek

Comprehensive analysis of the editing window of C-to-T TALE base editors

Autor: Maria Feola, Sylvain Pulicani, Diane Tkach, Alex Boyne, Robert Hong, Louisa Mayer, Aymeric Duclert, Philippe Duchateau, Alexandre Juillerat

Publikováno v: Scientific Reports, Vol 14, Iss 1, Pp 1-10 (2024)

Abstract One of the most recent advances in the genome editing field has been the addition of “TALE Base Editors”, an innovative platform for cell therapy that relies on the deamination of cytidines within double strand DNA, leading to the format

Externí odkaz: https://doaj.org/article/1c9ad18707b24a4ba4d27a874de706cd

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Akademický článek

Optimized two‐step electroporation process to achieve efficient nonviral‐mediated gene insertion into primary T cells

Autor: Ming Yang, Diane Tkach, Alex Boyne, Selena Kazancioglu, Aymeric Duclert, Laurent Poirot, Philippe Duchateau, Alexandre Juillerat

Publikováno v: FEBS Open Bio, Vol 12, Iss 1, Pp 38-50 (2022)

The development of gene editing technologies over the past years has allowed the precise and efficient insertion of transgenes into the genome of various cell types. Knock‐in approaches using homology‐directed repair and designer nucleases often

Externí odkaz: https://doaj.org/article/b489919778d241b5b206cd7cf268dbe0

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Akademický článek

Efficient multitool/multiplex gene engineering with TALE-BE

Autor: Alex Boyne, Ming Yang, Sylvain Pulicani, Maria Feola, Diane Tkach, Robert Hong, Aymeric Duclert, Philippe Duchateau, Alexandre Juillerat

Publikováno v: Frontiers in Bioengineering and Biotechnology, Vol 10 (2022)

TALE base editors are a recent addition to the genome editing toolbox. These molecular tools are fusions of a transcription activator-like effector domain (TALE), split-DddA deaminase halves, and an uracil glycosylase inhibitor (UGI) that have the di

Externí odkaz: https://doaj.org/article/5859141248d2427197bdff48d14a5260

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Akademický článek

Straightforward Generation of Ultrapure Off-the-Shelf Allogeneic CAR-T Cells

Autor: Alexandre Juillerat, Diane Tkach, Ming Yang, Alex Boyne, Julien Valton, Laurent Poirot, Philippe Duchateau

Publikováno v: Frontiers in Bioengineering and Biotechnology, Vol 8 (2020)

Here, we developed a straightforward methodology to generate TCRαβ negative (allogeneic) cells for CAR-T cell therapies. With an early and transient expression of an anti-CD3 CAR in the engineered donor T cells, we programmed these cells to self-el

Externí odkaz: https://doaj.org/article/eb8c501e33ee4766a40525071518c327

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Akademický článek

Molecular signature of high yield (growth) influenza a virus reassortants prepared as candidate vaccine seeds.

Autor: Manojkumar Ramanunninair, Jianhua Le, Shiroh Onodera, Andrew A Fulvini, Barbara A Pokorny, Jeanmarie Silverman, Rene Devis, Jennifer M Arroyo, Yu He, Alex Boyne, Jayati Bera, Rebecca Halpin, Erin Hine, David J Spiro, Doris Bucher

Publikováno v: PLoS ONE, Vol 8, Iss 6, p e65955 (2013)

BackgroundHuman influenza virus isolates generally grow poorly in embryonated chicken eggs. Hence, gene reassortment of influenza A wild type (wt) viruses is performed with a highly egg adapted donor virus, A/Puerto Rico/8/1934 (PR8), to provide the

Externí odkaz: https://doaj.org/article/53196e23cc3f471daacefb07e938b4e1

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Akademický článek

Genomic and protein structural maps of adaptive evolution of human influenza A virus to increased virulence in the mouse.

Publikováno v: PLoS ONE, Vol 6, Iss 6, p e21740 (2011)

Adaptive evolution is characterized by positive and parallel, or repeated selection of mutations. Mouse adaptation of influenza A virus (IAV) produces virulent mutants that demonstrate positive and parallel evolution of mutations in the hemagglutinin

Externí odkaz: https://doaj.org/article/88f6ee287fae477495f74768ad678e77

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Optimized two‐step electroporation process to achieve efficient nonviral‐mediated gene insertion into primary T cells

Autor: Laurent Poirot, Alexandre Juillerat, Diane Tkach, Ming Yang, Philippe Duchateau, Selena Kazancioglu, Aymeric Duclert, Alex Boyne

Publikováno v: FEBS Open Bio, Vol 12, Iss 1, Pp 38-50 (2022)
FEBS Open Bio

Gene editing enables scientists to make precise changes to the genome of an organism using the cell’s own ability to repair damaged DNA using a supplied DNA template. In recent years, gene editing has been applied clinically in the treatment of dis

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::19d48658e4280aca4614dcbe916de048
https://doaj.org/article/b489919778d241b5b206cd7cf268dbe0

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