Prospecting environmental mycobacteria: combined molecular approaches reveal unprecedented diversity.

Autor: Alessandra Pontiroli, Tanya T Khera, Brian B Oakley, Sam Mason, Scot E Dowd, Emma R Travis, Girum Erenso, Abraham Aseffa, Orin Courtenay, Elizabeth M H Wellington

Publikováno v: PLoS ONE, Vol 8, Iss 7, p e68648 (2013)

Environmental mycobacteria (EM) include species commonly found in various terrestrial and aquatic environments, encompassing animal and human pathogens in addition to saprophytes. Approximately 150 EM species can be separated into fast and slow growe

Externí odkaz: https://doaj.org/article/c151d4d3037d450faf4909d2fdc8efd7

An inter-laboratory validation of a real time PCR assay to measure host excretion of bacterial pathogens, particularly of Mycobacterium bovis.

Autor: Emma R Travis, William H Gaze, Alessandra Pontiroli, Francis P Sweeney, David Porter, Sam Mason, Matthew J C Keeling, Rebecca M Jones, Jason Sawyer, Alicia Aranaz, Elena Castellanos Rizaldos, Jennifer Cork, Richard J Delahay, Gavin J Wilson, R Glyn Hewinson, Orin Courtenay, Elizabeth M H Wellington

Publikováno v: PLoS ONE, Vol 6, Iss 11, p e27369 (2011)

Advances in the diagnosis of Mycobacterium bovis infection in wildlife hosts may benefit the development of sustainable approaches to the management of bovine tuberculosis in cattle. In the present study, three laboratories from two different countri

Externí odkaz: https://doaj.org/article/83b61a997d6f42988fd1b0eb178a5830

Fate of transgenic plant DNA in the environment

Autor: Alessandra Pontiroli, Åsa Frostegård, Pascal Simonet, Timothy M. Vogel, Jean-Michel Monier

Publikováno v: Environmental Biosafety Research
Environmental Biosafety Research, EDP Sciences, 2007, 6, pp.15-35. ⟨10.1051/ebr:2007037⟩

International audience; This review addresses the possible ecological effects of transgenic plants on micro-organisms in the field, hence, in the phytosphere and in the soil matrix. The important steps involved in the interaction between plant DNA an

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::ddf5cfb82ba19407e09d8e33db946616
https://doi.org/10.1051/ebr:2007037

Pathogen quantitation in complex matrices : a multi-operator comparison of DNA extraction methods with a novel assessment of PCR inhibition

Autor: Sam Mason, F. P. Sweeney, William H. Gaze, Alessandra Pontiroli, Jennifer M. Holden, Victoria Hibberd, Emma Rachel Travis, Elizabeth M. H. Wellington, Orin Courtenay, David Porter

Publikováno v: PLoS ONE
PLoS ONE, Vol 6, Iss 3, p e17916 (2011)

Background: Mycobacterium bovis is the aetiological agent of bovine tuberculosis (bTB), an important recrudescent zoonosis, significantly increasing in British herds in recent years. Wildlife reservoirs have been identified for this disease but the m

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::af06b0c0a2bb38864b1aa6de23a14f96
http://wrap.warwick.ac.uk/4547/9/WRAP_Wellington_0380313-lf-170511-pontiroli_cc_revised_2.pdf

Long-term persistence and bacterial transformation potential of transplastomic plant DNA in soil

Autor: Alessandra Pontiroli, Paolo Nannipieri, Maria Teresa Ceccherini, Timothy M. Vogel, Pascal Simonet, Walter Wildi, Elisabeth Kay, Jean-Michel Monier, John Poté

Publikováno v: Research in Microbiology
Research in Microbiology, Elsevier, 2010, 161 (5), pp.326-334. ⟨10.1016/j.resmic.2010.04.009⟩
Research in Microbiology, Elsevier, 2010, 161 (5), pp.326-334. 〈10.1016/j.resmic.2010.04.009〉

International audience; The long-term physical persistence and biological activity of transplastomic plant DNA (transgenes contained in the chloroplast genome) either purified and added to soil or naturally released by decaying tobacco leaves in soil

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::244bf1eeed8d802719e9e5a62ec09155
https://hal.archives-ouvertes.fr/hal-00539229