Zobrazeno 1 - 6
of 6
pro vyhledávání: '"Aleksei V. Yantsevich"'
Autor:
Sergei A. Usanov, Veronika V. Shchur, Yuliya P. Burankova, Aleksei V. Yantsevich, A. I. Zholnerovich
Publikováno v:
Proceedings of the National Academy of Sciences of Belarus, Chemical Series. 56:457-464
Publikováno v:
SLAS Technology. 24:556-568
An effective oligonucleotide preparation approach for the thermodynamically balanced, inside-out (TBIO) PCR-based assembly of long synthetic DNA molecules (synthons) is described in the current work. We replaced the necessity to purify individual oli
Autor:
Michail A. Shapira, Yuliya P. Burankova, Sergei A. Usanov, Veronika V. Shchur, Dmitry V. Klevzhits, Aleksei V. Yantsevich
Publikováno v:
Applied microbiology and biotechnology. 103(21-22)
Synthesis of custom de novo DNA sequences is highly demanded by fast-growing field of synthetic biology. Usually DNA sequences with length more than 1 kb are assembled from smaller synthetic DNA fragments (synthons) obtained by PCR assembly. The abil
Autor:
Andrei I. Zhauniarovich, Yuliya P. Burankova, Veronika V. Shchur, Sergei A. Usanov, Yaraslau V. Dzichenka, Aleksei V. Yantsevich
Publikováno v:
Analytical Biochemistry. 617:114115
The functionalization of 5ʹ-OH group in nucleic acids is of significant value for molecular biology. In the current work we discovered that acid-labile 4,4ʹ-dimethoxytrityl protecting group (DMT) of oligonucleotides (ONs) is stable under PCR condit
Publikováno v:
Biochemistry (Moscow). 72:77-83
In this article we report on construction of expression vector, heterologous expression in Escherichia coli, isolation, purification, and physicochemical characterization of an artificial chimeric protein HMWb(5)-EGFP consisting of full-length cytoch
Autor:
Natallia Strushkevich, Sergey A. Usanov, Aleksei V. Yantsevich, Farrell MacKenzie, Andrei A. Gilep, Yaroslav V. Dichenko, Alexander V. Baranovsky, Dmitry V. Mukha
Publikováno v:
The FEBS journal. 281(6)
Oxysterols and neurosteroids are important signaling molecules produced by monooxygenases of the cytochrome P450 family that realize their effect through nuclear receptors. CYP7B1 catalyzes the 6- or 7-hydroxylation of both steroids and oxysterols an