Zobrazeno 1 - 10
of 13
pro vyhledávání: '"Alejandro Vian"'
Autor:
David Muñoz-Rodríguez, Marwane Bourqqia-Ramzi, Maria Teresa García-Esteban, Antonio Murciano-Cespedosa, Alejandro Vian, Juan Lombardo-Hernández, Pablo García-Pérez, Francisco Conejero, Álvaro Mateos González, Stefano Geuna, Celia Herrera-Rincon
Publikováno v:
International Journal of Molecular Sciences, Vol 24, Iss 17, p 13394 (2023)
Inter-cellular communication is mediated by a sum of biochemical, biophysical, and bioelectrical signals. This might occur not only between cells belonging to the same tissue and/or animal species but also between cells that are, from an evolutionary
Externí odkaz:
https://doaj.org/article/02decf045b274f6d849332c039a64eee
Autor:
Alejandro Vian, Alfonso V. Carrascosa, Benevides C. C. Pessela, José Luis García, Jose M. Guisan, Rodrigo Torres, Roberto Munilla, Roberto Fernandez-Lafuente, Manuel Fuentes, Cesar Mateo
Publikováno v:
Journal of Chromatography A. 1055:93-98
A strategy to selectively adsorb large proteins on immobilized metal ion affinity chromatography supports is presented. It is based on the fact that large proteins have a large surface that permits the long distance interaction with groups placed qui
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::351201c53679f26cfc5cbe12f301d0bc
https://doi.org/10.1016/j.chroma.2004.08.141
https://doi.org/10.1016/j.chroma.2004.08.141
Autor:
Cesar Mateo, Alfonso V. Carrascosa, Alejandro Vian, Manuel Fuentes, José Luis García, Benevides C. C. Pessela, Jose M. Guisan, Roberto Fernandez-Lafuente
Publikováno v:
Enzyme and Microbial Technology. 33:199-205
The β-galactosidase from Thermus sp. T2 (Htag-BgaA) is competitively inhibited by galactose (3.1 mM) and non-competitively inhibited by glucose (49.9 mM). These inhibitions were strongly reduced by immobilization on heterofunctional epoxy Sepabeads
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::2b632892a7e730883a0d6d1f30ab3ad0
https://doi.org/10.1016/s0141-0229(03)00120-0
https://doi.org/10.1016/s0141-0229(03)00120-0
Autor:
Roberto Fernandez-Lafuente, Gloria Fernández-Lorente, Alfonso V. Carrascosa, José Luis García, Benevides C. C. Pessela, Jose M. Guisan, Cesar Mateo, Alejandro Vian
Publikováno v:
Journal of Chromatography A. 915:97-106
New immobilized metal ion affinity chromatography (IMAC) matrices containing a high concentration of metal–chelate moieties and completely coated with inert flexible and hydrophilic dextrans are here proposed to improve the purification of polyhist
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::7e812f7d3d20b14f4a357eeec37091b3
https://doi.org/10.1016/s0021-9673(01)00626-4
https://doi.org/10.1016/s0021-9673(01)00626-4
Autor:
Miguel A. Peñalva, Alejandro Vian
Publikováno v:
Current Genetics. 17:223-227
We have cloned the Cephalosporium acremonium pyr4 gene by cross-hybridization with the equivalent gene from Neurospora crassa, the closest relative from which this gene is available. The C. acremonium pyr4 gene complements an E. coli pyrF mutant lack
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::8c7732130f98354cd72c97608c20c3db
https://doi.org/10.1007/bf00312613
https://doi.org/10.1007/bf00312613
Autor:
Benevides C C, Pessela, Rodrigo, Torres, Manuel, Fuentes, Cesar, Mateo, Roberto, Munilla, Alejandro, Vian, Alfonso V, Carrascosa, Jose L c, Garcia, Jose M, Guisán, Roberto, Fernandez-Lafuente
Publikováno v:
Journal of chromatography. A. 1055(1-2)
A strategy to selectively adsorb large proteins on immobilized metal ion affinity chromatography supports is presented. It is based on the fact that large proteins have a large surface that permits the long distance interaction with groups placed qui
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=pmid________::59cf4f3d9b4488a75f8974435d35da4c
https://pubmed.ncbi.nlm.nih.gov/15560484
https://pubmed.ncbi.nlm.nih.gov/15560484
Autor:
Jose M. Guisan, Alejandro Vian, Roberto Fernandez-Lafuente, Manuel Fuentes, Alfonso V. Carrascosa, Roberto Munilla, Benevides C. C. Pessela, Lorena Betancor
Publikováno v:
Journal of chromatography. A. 1034(1-2)
Ion-exchange chromatography using commercial ionic supports is a commonly used technique for protein purification. However, selective adsorption of a target protein from a given extract onto commercial ion exchangers seems to be quite complex since t
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::901a22752ce556d5bc500ff3c8340705
https://pubmed.ncbi.nlm.nih.gov/15116925
https://pubmed.ncbi.nlm.nih.gov/15116925
Autor:
Cesar Mateo, Alejandro Vian, Alfonso V. Carrascosa, Roberto Fernandez-Lafuente, José Luis García, Benevides C. C. Pessela, Manuel Fuentes, Jose M. Guisan
Publikováno v:
Digital.CSIC. Repositorio Institucional del CSIC
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This work exemplifies the advantages of using a battery of new heterofunctional epoxy supports to immobilize enzymes. We have compared the performance of a standard Sepabeads-epoxy support with other Sepabeads-epoxy supports partially modified with b
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::be757d59c4479275438b9d848419c0b5
http://hdl.handle.net/10261/73796
http://hdl.handle.net/10261/73796
Autor:
Alfonso V. Carrascosa, Cesar Mateo, José Luis García, Benevides C. C. Pessela, Roberto Fernandez-Lafuente, Alejandro Vian, Jose M. Guisan
Publikováno v:
Applied and environmental microbiology. 69(4)
A novel thermostable chimeric β-galactosidase was constructed by fusing a poly-His tag to the N-terminal region of the β-galactosidase from Thermus sp. strain T2 to facilitate its overexpression in Escherichia coli and its purification by immobiliz
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::6f009aca3de46158dc7496f8d0f9b6c8
https://pubmed.ncbi.nlm.nih.gov/12676671
https://pubmed.ncbi.nlm.nih.gov/12676671
Autor:
Cesar Mateo, Benevides C. C. Pessela, Germán Rivas, Alejandro Vian, Carlos Alfonso, Roberto Fernandez-Lafuente, Jose M. Guisan, Alfonso V. Carrascosa, José Luis García
Publikováno v:
Biomacromolecules. 4(1)
Using the poly-His-tagged-beta-galactosidase from Thermus sp. strain T2 overexpressed in Escherichia coli (MC1116) as a model enzyme, we have developed a strategy to purify and immobilize proteins in a single step, combining the excellent properties
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::44f8cdf7719d3ce5c9737edd70e65901
https://pubmed.ncbi.nlm.nih.gov/12523854
https://pubmed.ncbi.nlm.nih.gov/12523854